Serial microbubble imaging technology (sMBI) for rapid screening of hydrogen-evolution materials used in photocatalytic water-splitting reactions

2017 ◽  
Vol 9 (12) ◽  
pp. 1835-1838
Author(s):  
Jiarui Zhang ◽  
Jianchao Lee ◽  
Liping Wang ◽  
Yunyun Zheng ◽  
Wenxiao Wang ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
Water Splitting ◽  
High Throughput ◽  
High Throughput Screening ◽  
Detection Method ◽  
Rapid Screening ◽  
Imaging Technology ◽  
Photocatalytic Water Splitting ◽  
Materials Used

A new detection method, serial microbubble imaging (sMBI) was developed for high-throughput screening of thousands of H2-evolution catalysts.

scholarly journals Acetylene-linked conjugated polymers for sacrificial photocatalytic hydrogen evolution from water

2021 ◽  
Author(s):  
Lunjie Liu ◽  
Michał Kochman ◽  
Yongjie Xu ◽  
Martijn Zwijnenburg ◽  
Andrew Cooper ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Conjugated Polymers ◽  
Hydrogen Evolution ◽  
Water Splitting ◽  
High Throughput ◽  
High Throughput Screening ◽  
Organic Polymers ◽  
Photocatalytic Hydrogen Evolution ◽  
Conjugated Organic Polymers

Conjugated organic polymers have shown potential as photocatalysts for hydrogen production by water splitting. Taking advantage of a high throughput screening workflow, two series of acetylene-linked co-polymers were prepared and...

scholarly journals A high throughput optical method for studying compositional effects in electrocatalysts for CO2 reduction

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jeremy L. Hitt ◽  
Yuguang C. Li ◽  
Songsheng Tao ◽  
Zhifei Yan ◽  
Yue Gao ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Screening Method ◽  
Co2 Reduction ◽  
Fold Increase ◽  
Faradaic Efficiency ◽  
Atomic Pair Distribution Function ◽  
Earth Abundant ◽  
Ternary Catalysts

AbstractIn the problem of electrochemical CO2 reduction, the discovery of earth-abundant, efficient, and selective catalysts is essential to enabling technology that can contribute to a carbon-neutral energy cycle. In this study, we adapt an optical high throughput screening method to study multi-metallic catalysts for CO2 electroreduction. We demonstrate the utility of the method by constructing catalytic activity maps of different alloyed elements and use X-ray scattering analysis by the atomic pair distribution function (PDF) method to gain insight into the structures of the most active compositions. Among combinations of four elements (Au, Ag, Cu, Zn), Au6Ag2Cu2 and Au4Zn3Cu3 were identified as the most active compositions in their respective ternaries. These ternary electrocatalysts were more active than any binary combination, and a ca. 5-fold increase in current density at potentials of −0.4 to −0.8 V vs. RHE was obtained for the best ternary catalysts relative to Au prepared by the same method. Tafel plots of electrochemical data for CO2 reduction and hydrogen evolution indicate that the ternary catalysts, despite their higher surface area, are poorer catalysts for the hydrogen evolution reaction than pure Au. This results in high Faradaic efficiency for CO2 reduction to CO.

High-Throughput Screening of Hydrogen Evolution Reaction Catalysts in MXene Materials

2020 ◽  
Vol 124 (25) ◽  
pp. 13695-13705 ◽  
Author(s):  
Jingnan Zheng ◽  
Xiang Sun ◽  
Chenglong Qiu ◽  
Yilong Yan ◽  
Zihao Yao ◽  
...  
Keyword(s):  
Hydrogen Evolution ◽  
High Throughput ◽  
Hydrogen Evolution Reaction ◽  
High Throughput Screening

Development of a dual-fluorescence reporter system for high-throughput screening of L-aspartate-α-decarboxylase

2020 ◽  
Vol 52 (12) ◽  
pp. 1420-1426
Author(s):  
Mingyue Fei ◽  
Xudan Mao ◽  
Yiyang Chen ◽  
Yalan Lu ◽  
Lin Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Industrial Production ◽  
High Throughput Screening ◽  
Relative Activity ◽  
Expression Level ◽  
Rapid Screening ◽  
Dual Fluorescence ◽  
Reporter System ◽  
The Cost ◽  
Chemical Synthesis Route

Abstract β-Alanine (3-aminopropionic acid) holds great potential in industrial application. It can be obtained through a chemical synthesis route, which is hazardous to the environment. It is well known that l-aspartate-α-decarboxylase (ADC) can convert l-aspartate to β-alanine in bacteria. However, due to the low activity of ADC, industrial production of β-alanine through the green biological route remains unclear. Thus, improving the activity of ADC is critical to reduce the cost of β-alanine production. In this study, we established a dual-fluorescence high-throughput system for efficient ADC screening. By measuring the amount of β-alanine and the expression level of ADC using two different fluorescence markers, we can rapidly quantify the relative activity of ADC variants. From a mutagenesis library containing 2000 ADC variants, we obtained a mutant with 33% increased activity. Further analysis revealed that mutations of K43R and P103Q in ADC significantly improved the yield of β-alanine produced by the whole-cell biocatalysis. Compared with the previous single-fluorescence method, our system can not only quantify the amount of β-alanine but also measure the expression level of ADC with different fluorescence, making it able to effectively screen out ADC variants with improved relative activity. The dual-fluorescence high-throughput system for rapid screening of ADC provides a good strategy for industrial production of β-alanine via the biological conversion route in the future.

Fluorescence imaging technology (FI) for high-throughput screening of selenide-modified nano-TiO2 catalysts

2016 ◽  
Vol 52 (14) ◽  
pp. 2944-2947 ◽  
Author(s):  
Liping Wang ◽  
Jianchao Lee ◽  
Meijuan Zhang ◽  
Qiannan Duan ◽  
Jiarui Zhang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
High Throughput ◽  
High Throughput Screening ◽  
Catalytic Performance ◽  
Nano Tio2 ◽  
Printing Technology ◽  
Imaging Technology ◽  
Ink Jet Printing ◽  
Ink Jet ◽  
Jet Printing

A high-throughput screening (HTS) method based on fluorescence imaging (FI) was built and applied to evaluate the catalytic performance of selenides-modified TiO2. A catalyst library comprising 1405 catalysts was established using color ink-jet printing technology.

scholarly journals Recovery of NanoLuc Luciferase-Tagged Canine Distemper Virus for Facilitating Rapid Screening of Antivirals in vitro

2020 ◽  
Vol 7 ◽  
Author(s):  
Fuxiao Liu ◽  
Qianqian Wang ◽  
Yilan Huang ◽  
Ning Wang ◽  
Youming Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Canine Distemper Virus ◽  
Reverse Genetics ◽  
Rapid Screening ◽  
Canine Distemper ◽  
Virus Propagation ◽  
The Family ◽  
Conventional Methods

Canine distemper virus (CDV), belonging to the genus Morbillivirus in the family Paramyxoviridae, is a highly contagious pathogen, affecting various domestic, and wild carnivores. Conventional methods are too cumbersome to be used for high-throughput screening of anti-CDV drugs. In this study, a recombinant CDV was rescued using reverse genetics for facilitating screening of anti-CDV drug in vitro. The recombinant CDV could stably express the NanoLuc® luciferase (NLuc), a novel enzyme that was smaller and “brighter” than others. The intensity of NLuc-catalyzed luminescence reaction indirectly reflected the anti-CDV effect of a certain drug, due to a positive correlation between NLuc expression and virus propagation in vitro. Based on such a characteristic feature, the recombinant CDV was used for anti-CDV assays on four drugs (ribavirin, moroxydine hydrochloride, 1-adamantylamine hydrochloride, and tea polyphenol) via analysis of luciferase activity, instead of via conventional methods. The result showed that out of these four drugs, only the ribavirin exhibited a detectable anti-CDV effect. The NLuc-tagged CDV would be a rapid tool for high-throughput screening of anti-CDV drugs.

scholarly journals Mechanism for spontaneous oxygen and hydrogen evolution reactions on CoO nanoparticles

2019 ◽  
Vol 7 (12) ◽  
pp. 6708-6719 ◽  
Author(s):  
Kyoung-Won Park ◽  
Alexie M. Kolpak
Keyword(s):  
Hydrogen Evolution ◽  
Water Splitting ◽  
High Efficiency ◽  
Applied Bias ◽  
Co Catalyst ◽  
Photocatalytic Water Splitting ◽  
Overall Water Splitting ◽  
Nanoparticle Suspensions ◽  
Hydrogen Evolution Reactions ◽  
Coo Nanoparticles

Overall photocatalytic water splitting with a high efficiency has recently been observed for CoO nanoparticle suspensions in the absence of an applied bias or co-catalyst. This study clarifies the mechanism of spontaneous overall water splitting with the prominent efficiency observed on the CoO nanoparticle.

scholarly journals Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms

2020 ◽  
Vol 21 (9) ◽  
pp. 3034 ◽  
Author(s):  
Shella Gilbert-Girard ◽  
Kirsi Savijoki ◽  
Jari Yli-Kauhaluoma ◽  
Adyary Fallarero
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
High Throughput ◽  
High Throughput Screening ◽  
Bacterial Infections ◽  
Rapid Screening ◽  
Bacterial Biofilms ◽  
Main Concern ◽  
Assay Optimization ◽  
Screening Platform

In recent years, bacterial infections have become a main concern following the spread of antimicrobial resistance. In addition, bacterial biofilms are known for their high tolerance to antimicrobials and they are regarded as a main cause of recalcitrant infections in humans. Many efforts have been deployed in order to find new antibacterial therapeutic options and the high-throughput screening (HTS) of large libraries of compounds is one of the utilized strategies. However, HTS efforts for anti-biofilm discovery remain uncommon. Here, we miniaturized a 96-well plate (96WP) screening platform, into a 384-well plate (384WP) format, based on a sequential viability and biomass measurements for the assessment of anti-biofilm activity. During the assay optimization process, different parameters were evaluated while using Staphylococcus aureus and Pseudomonas aeruginosa as the bacterial models. We compared the performance of the optimized 384WP platform to our previously established 96WP-based platform by carrying out a pilot screening of 100 compounds, followed by the screening of a library of 2000 compounds to identify new repurposed anti-biofilm agents. Our results show that the optimized 384WP platform is well-suited for screening purposes, allowing for the rapid screening of a higher number of compounds in a run in a reliable manner.

scholarly journals A Novel High-Throughput Screening Assay for Sickle Cell Disease Drug Discovery

2009 ◽  
Vol 14 (4) ◽  
pp. 330-336 ◽  
Author(s):  
Eszter Pais ◽  
John S. Cambridge ◽  
Cage S. Johnson ◽  
Herbert J. Meiselman ◽  
Timothy C. Fisher ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Rapid Screening ◽  
Cell Disease ◽  
Screening Assay ◽  
Drug Candidates ◽  
Test Molecule ◽  
High Throughput Screening Assay

Although the pathophysiology and molecular basis of sickle cell disease (SCD) were described more than half a century ago, an effective and safe therapy is not yet available. This may be explained by the lack of a suitable high-throughput technique that allows rapid screening of thousands of compounds for their antisickling effect. The authors have thus developed a novel high-throughput screening (HTS) assay based on detecting the ability of red blood cells (RBC) to traverse a column of tightly packed Sephacryl chromatography beads. When deoxygenated, sickle RBC are rigid and remain on the top of the column. However, when deoxygenated and treated with an effective antisickling agent, erythrocytes move through the Sephacryl media and produce a red dot on the bottom of the assay tubes. This approach has been adapted to wells in a 384-well microplate. Results can be obtained by optical scanning: The size of the red dot is proportional to the antisickling effect of the test molecule. The new assay is simple, inexpensive, reproducible, requires no special reagents, and should be readily adaptable to robotic HTS systems. It has the potential to identify novel drug candidates, allowing the development of new therapeutic options for individuals affected with SCD. ( Journal of Biomolecular Screening. 2009:330-336)

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