A novel, label-free fluorescent aptasensor for cocaine detection based on a G-quadruplex and ruthenium polypyridyl complex molecular light switch

2016 ◽  
Vol 8 (18) ◽  
pp. 3740-3746 ◽  
Author(s):  
Songbai Zhang ◽  
Linping Wang ◽  
Meiling Liu ◽  
Yanqing Qiu ◽  
Mengna Wang ◽  
...  
Keyword(s):  
High Selectivity ◽  
Label Free ◽  
Ruthenium Polypyridyl ◽  
Polypyridyl Complex ◽  
G Quadruplex ◽  
Molecular Light Switch ◽  
Cocaine Detection ◽  
Fluorescent Aptasensor

A [Ru(bpy)2(bqdppz)]2+ ligand with high selectivity towards G-quadruplex was synthesized and introduced to serve as a prominent molecular light switch via specifically recognizing the cocaine aptamer-involved G-quadruplex.

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen

The Analyst ◽  
2021 ◽  
Author(s):  
Ruirui Zhao ◽  
Lu Zhao ◽  
Haidi Feng ◽  
Xiaoliang Chen ◽  
Huilin Zhang ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sensing Platforms ◽  
G Quadruplex ◽  
Fluorescent Aptasensor

Fluorescence sensing platforms based on HCR and G-quadruplex DNAzyme amplification strategies for the detection of prostate-specific antigen.

scholarly journals A Label-Free Fluorescent Assay for the Rapid and Sensitive Detection of Adenosine Deaminase Activity and Inhibition

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2441 ◽  
Author(s):  
Xinxing Tang ◽  
Kefeng Wu ◽  
Han Zhao ◽  
Mingjian Chen ◽  
Changbei Ma
Keyword(s):  
Adenosine Deaminase ◽  
High Selectivity ◽  
High Sensitivity ◽  
Rnase H ◽  
Fluorescence Signal ◽  
Label Free ◽  
Fluorescent Assay ◽  
Adenosine Deaminase Activity ◽  
G Quadruplex ◽  
Almost All

Adenosine deaminase (ADA), able to catalyze the irreversible deamination of adenosine into inosine, can be found in almost all tissues and plays an important role in several diseases. In this work, we developed a label-free fluorescence method for the detection of adenosine deaminase activity and inhibition. In the presence of ADA, ATP has been shown to be hydrolyzed. The ATP aptamer was shown to form a G-quadruplex/thioflavin T (ThT) complex with ThT and exhibited an obvious fluorescence signal. However, the ATP aptamer could bind with ATP and exhibited a low fluorescence signal because of the absence of ADA. This assay showed high sensitivity to ADA with a detection limit of 1 U/L based on an SNR of 3 and got a good linear relationship within the range of 1–100 U/L with R2 = 0.9909. The LOD is lower than ADA cutoff value (4 U/L) in the clinical requirement and more sensitive than most of the reported methods. This technique exhibited high selectivity for ADA against hoGG I, UDG, RNase H and λexo. Moreover, this strategy was successfully applied for assaying the inhibition of ADA using erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) and, as such, demonstrated great potential for the future use in the diagnosis of ADA-relevant diseases, particularly in advanced drug development.

Visual specific luminescent probing of hybrid G-quadruplex DNA by a ruthenium polypyridyl complex

2012 ◽  
Vol 48 (87) ◽  
pp. 10781 ◽  
Author(s):  
Guo-Liang Liao ◽  
Xiang Chen ◽  
Liang-Nian Ji ◽  
Hui Chao
Keyword(s):  
Ruthenium Polypyridyl ◽  
Quadruplex Dna ◽  
Polypyridyl Complex ◽  
G Quadruplex

scholarly journals Structural Studies Reveal Enantiospecific Recognition of a DNA G‐Quadruplex by a Ruthenium Polypyridyl Complex

2019 ◽  
Vol 58 (29) ◽  
pp. 9881-9885 ◽  
Author(s):  
Kane McQuaid ◽  
Holly Abell ◽  
Sarah P. Gurung ◽  
David R. Allan ◽  
Graeme Winter ◽  
...  
Keyword(s):  
Structural Studies ◽  
Ruthenium Polypyridyl ◽  
Polypyridyl Complex ◽  
G Quadruplex

scholarly journals Structural Studies Reveal Enantiospecific Recognition of a DNA G‐Quadruplex by a Ruthenium Polypyridyl Complex

2019 ◽  
Vol 131 (29) ◽  
pp. 9986-9990 ◽  
Author(s):  
Kane McQuaid ◽  
Holly Abell ◽  
Sarah P. Gurung ◽  
David R. Allan ◽  
Graeme Winter ◽  
...  
Keyword(s):  
Structural Studies ◽  
Ruthenium Polypyridyl ◽  
Polypyridyl Complex ◽  
G Quadruplex

scholarly journals Label-Free Fluorescent Aptasensor for Ochratoxin—A Detection Based on CdTe Quantum Dots and (N-Methyl-4-pyridyl) Porphyrin

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 447 ◽  
Author(s):  
Li Liu ◽  
Zafar Iqbal Tanveer ◽  
Keqiu Jiang ◽  
Qingwen Huang ◽  
Jinghui Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Ochratoxin A ◽  
Cdte Quantum Dots ◽  
Random Coil ◽  
Label Free ◽  
Promising Tool ◽  
Cdte Qds ◽  
G Quadruplex ◽  
Sensitive Determination ◽  
Fluorescent Aptasensor

With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL−1, with a linear range of 0.2 to 20 ng mL−1. Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied in Astragalus membranaceus samples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.

A luminescent G-quadruplex-selective iridium(iii) complex for the label-free detection of lysozyme

2016 ◽  
Vol 4 (14) ◽  
pp. 2407-2411 ◽  
Author(s):  
Lihua Lu ◽  
Wanhe Wang ◽  
Modi Wang ◽  
Tian-Shu Kang ◽  
Jin-Jian Lu ◽  
...  
Keyword(s):  
High Selectivity ◽  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

A novel Ir(iii) complex 1 displays high selectivity for the G-quadruplex, and was used to establish a label-free G-quadruplex-based detection platform for lysozyme in buffer.

A facile label-free G-quadruplex based fluorescent aptasensor method for rapid detection of ATP

2017 ◽  
Vol 175 ◽  
pp. 164-167 ◽  
Author(s):  
Haisheng Liu ◽  
Changbei Ma ◽  
Feng Ning ◽  
Hanchun Chen ◽  
Hailun He ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Label Free ◽  
G Quadruplex ◽  
Fluorescent Aptasensor

scholarly journals Label-Free Electrochemical Test of Protease Interaction with a Peptide Substrate Modified Gold Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 199
Author(s):  
Anna Wcisło ◽  
Izabela Małuch ◽  
Paweł Niedziałkowski ◽  
Tadeusz Ossowski ◽  
Adam Prahl
Keyword(s):  
Gold Electrode ◽  
High Selectivity ◽  
Electrode Materials ◽  
Electrochemical Biosensors ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Peptide Substrate ◽  
Electrochemical Test ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method

Efficient deposition of biomolecules on the surface, maintaining their full activity and stability, is a most significant factor in biosensor construction. For this reason, more and more research is focused on the development of electrochemical biosensors that have the ability to electrically detect adsorbed molecules on electrode surface with high selectivity and sensitivity. The presented research aims to develop an efficient methodology that allows quantification of processes related to the evaluation of enzyme activity (proprotein convertase) using electrochemical methods. In this study we used impedance spectroscopy to investigate the immobilization of peptide substrate (Arg-Val-Arg-Arg) modified with 11-mercaptoundecanoic acid on the surface of gold electrode. Both the synthesis of the peptide substrate as well as the full electrochemical characteristics of the obtained electrode materials have been described. Experimental conditions, including concentration of peptide substrate immobilization, modification time, linker, and the presence of additional blocking groups have been optimized. The main advantages of the described method is that it makes it possible to observe the peptide substrate–enzyme interaction without the need to use fluorescent labels. This also allows observation of this interaction at a very low concentration. Both of these factors make this new technique competitive with the standard spectrofluorimetric method.

Sign in / Sign up

Export Citation Format

Share Document