A new approach to rapid determination of 18 bioactive constituents in Alpinia oxyphylla using UPLC-MS/MS with positive–negative conversion multiple reaction monitor (+/−MRM) technology

2016 ◽  
Vol 8 (15) ◽  
pp. 3163-3170 ◽  
Author(s):  
Lei Hou ◽  
Lihua Zuo ◽  
Zhi Sun ◽  
Zhiyong Sun ◽  
Zhenfeng Zhu ◽  
...  
Keyword(s):  
Rapid Determination ◽  
Bioactive Constituents ◽  
Active Components ◽  
New Approach ◽  
Alpinia Oxyphylla ◽  
Simultaneous Separation ◽  
Negative Conversion

A new approach was developed for the simultaneous separation and determination of 18 active components (including one new component) in Fructus Alpinia oxyphylla by UPLC-MS/MS with +/−MRM technology.

A combination of near infrared and mid-infrared spectroscopy to improve the determination efficiency of active components in Radix Astragali

2019 ◽  
Vol 28 (1) ◽  
pp. 10-17
Author(s):  
Xuesong Liu ◽  
Siyu Zhang ◽  
Leting Si ◽  
Zhonglin Lin ◽  
Chunyan Wu ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared ◽  
Rapid Determination ◽  
Radix Astragali ◽  
Astragaloside Iv ◽  
Active Components ◽  
Mid Infrared ◽  
Mid Infrared Spectroscopy ◽  
Analysis Models

Radix Astragali is a popular herbal medicine in the pharmaceutical industry, and many studies have confirmed its significant medical value. The objective of this study was to achieve rapid determination of the main active components (astragaloside IV and total astragalosides) in Radix Astragali using near infrared spectroscopy, mid-infrared spectroscopy, and a combination of them. Partial least squares regression was applied to establish quantitative calibration analysis models. According to the prediction results, the combined near infrared and mid-infrared spectroscopy performed better than near infrared and mid-infrared models individually. The determination coefficient and root mean square error of prediction of astragaloside IV and total astragalosides were 0.998, 0.025 and 0.998, 0.098, respectively. It can be concluded that quantitative analysis models constructed by combined near infrared and mid-infrared spectroscopy had superior performance and could be used for rapid determination of main active components in Radix Astragali.

scholarly journals A “Green” Homogenate Extraction Coupled with UHPLC-MS for the Rapid Determination of Diterpenoids in Croton Crassifolius

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 694
Author(s):  
Jin-Long Tian ◽  
Chi Shu ◽  
Ye Zhang ◽  
Hui-Jun Cui ◽  
Xu Xie ◽  
...  
Keyword(s):  
High Performance ◽  
Rapid Determination ◽  
Biological Activities ◽  
Bioactive Constituents ◽  
Complex Species ◽  
Chromatography Tandem Mass Spectrometry ◽  
Homogenate Extraction ◽  
Liquid Chromatography Tandem Mass ◽  
Clerodane Diterpenoids

Clerodane diterpenoids are the main bioactive constituents of Croton crassifolius and are proved to have multiple biological activities. However, quality control (QC) research on the constituents are rare. Thus, the major research purpose of the current study was to establish an efficient homogenate extraction (HGE) process combined with a sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS) technique together for the rapid extraction and determination of clerodane diterpenoids in C. crassifolius. All calibration curves showed good linearity (r > 0.9943) within the test ranges and the intra- and inter-day precisions and repeatability were all within required limits. This modified HGE–UHPLC–MS method only took 5 min to extract nine clerodane diterpenoids in C. crassifolius and another 12 min to quantify these components. The results indicated that the quantitative analysis based on UHPLC–MS was a feasible method for QC of clerodane diterpenoids in C. crassifolius, and the findings outlined in the current study also inferred the potential of the method in the QC of clerodane diterpenoids in other complex species of plants.

A New Approach to the Rapid Determination of Protein Side Chain Conformations

1991 ◽  
Vol 8 (6) ◽  
pp. 1267-1289 ◽  
Author(s):  
P. Tuffery ◽  
C. Etchebest ◽  
S. Hazout ◽  
R. Lavery
Keyword(s):  
Rapid Determination ◽  
Side Chain ◽  
New Approach ◽  
Chain Conformations

scholarly journals Quality Evaluation of Wild and Cultivated Schisandrae Chinensis Fructus Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1335 ◽  
Author(s):  
Shuyu Chen ◽  
Jingjing Shi ◽  
Lisi Zou ◽  
Xunhong Liu ◽  
Renmao Tang ◽  
...  
Keyword(s):  
Organic Acids ◽  
Simultaneous Determination ◽  
Malic Acid ◽  
Protocatechuic Acid ◽  
Quinic Acid ◽  
Gray Relational Analysis ◽  
Bioactive Constituents ◽  
Active Components

Schisandrae Chinensis Fructus, also called wuweizi in China, was a widely used folk medicine in China, Korea, and Russia. Due to the limited natural resources and huge demand of wuweizi, people tend to cultivate wuweizi to protect this species. However, the quality of wild and cultivated herbs of the same species may change. Little attention has been paid to comparing wild and cultivated wuweizi based on simultaneous determination of its active components, such as lignans and organic acids. An analytical method based on UFLC-QTRAP-MS/MS was used for the simultaneous determination of 15 components, including 11 lignans (schisandrin, gomisin D, gomisin J, schisandrol B, angeloylgomisin H, schizantherin B, schisanhenol, deoxyschizandrin, γ-schisandrin, schizandrin C, and schisantherin) and 4 organic acids (quinic acid, d(−)-tartaric acid, l-(−)-malic acid, and protocatechuic acid) in wuweizi under different ecological environments. Principal components analysis (PCA), partial least squares discrimination analysis (PLS-DA), independent sample t-test, and gray relational analysis (GRA) have been applied to classify and evaluate samples from different ecological environments according to the content of 15 components. The results showed that the differential compounds (i.e., quinic acid, l-(−)-malic acid, protocatechuic acid, schisandrol B) were significantly related to the classification of wild and cultivated wuweizi. GRA results demonstrated that the quality of cultivated wuweizi was not as good as wild wuweizi. The protocol not just provided a new method for the comprehensive evaluation and quality control of wild and cultivated wuweizi, but paved the way to differentiate them at the chemistry level.

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