scholarly journals Quality Evaluation of Wild and Cultivated Schisandrae Chinensis Fructus Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1335 ◽  
Author(s):  
Shuyu Chen ◽  
Jingjing Shi ◽  
Lisi Zou ◽  
Xunhong Liu ◽  
Renmao Tang ◽  
...  
Keyword(s):  
Organic Acids ◽  
Simultaneous Determination ◽  
Malic Acid ◽  
Protocatechuic Acid ◽  
Quinic Acid ◽  
Gray Relational Analysis ◽  
Bioactive Constituents ◽  
Active Components

Schisandrae Chinensis Fructus, also called wuweizi in China, was a widely used folk medicine in China, Korea, and Russia. Due to the limited natural resources and huge demand of wuweizi, people tend to cultivate wuweizi to protect this species. However, the quality of wild and cultivated herbs of the same species may change. Little attention has been paid to comparing wild and cultivated wuweizi based on simultaneous determination of its active components, such as lignans and organic acids. An analytical method based on UFLC-QTRAP-MS/MS was used for the simultaneous determination of 15 components, including 11 lignans (schisandrin, gomisin D, gomisin J, schisandrol B, angeloylgomisin H, schizantherin B, schisanhenol, deoxyschizandrin, γ-schisandrin, schizandrin C, and schisantherin) and 4 organic acids (quinic acid, d(−)-tartaric acid, l-(−)-malic acid, and protocatechuic acid) in wuweizi under different ecological environments. Principal components analysis (PCA), partial least squares discrimination analysis (PLS-DA), independent sample t-test, and gray relational analysis (GRA) have been applied to classify and evaluate samples from different ecological environments according to the content of 15 components. The results showed that the differential compounds (i.e., quinic acid, l-(−)-malic acid, protocatechuic acid, schisandrol B) were significantly related to the classification of wild and cultivated wuweizi. GRA results demonstrated that the quality of cultivated wuweizi was not as good as wild wuweizi. The protocol not just provided a new method for the comprehensive evaluation and quality control of wild and cultivated wuweizi, but paved the way to differentiate them at the chemistry level.

scholarly journals Simultaneous Determination of Four Ingredients in Plantago Depressa by Single Marker

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yang Xu ◽  
Yuejie Wang ◽  
Shengnan Bi ◽  
Yanxue Jia ◽  
Huiwei Bao
Keyword(s):  
Simultaneous Determination ◽  
Standard Method ◽  
Protocatechuic Acid ◽  
Correction Factors ◽  
Active Components ◽  
Relative Correction ◽  
External Standard Method ◽  
External Standard ◽  
Single Marker

Objective. To establish a quantitative analysis of multicomponents by single marker (QAMS) method for the simultaneous determination of 4 active components such as protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa. Method. 4 active components in Plantago depressa were studied. Quercetin was used as an internal reference to establish the relative correction factors among protocatechuic acid, catechin, and luteolin and calculate the contents of each component; the results were compared with those measured by the external standard method. Results. 4 components showed a good linear relationship in their respective concentration ranges (r > 0.9995). The relative correction factors (fs/k) of protocatechuic acid, catechin, and luteolin were 1.1992, 0.8613, and 1.6069, respectively. The method had good durability. The contents of protocatechuic acid, catechin, and luteolin calculated by QAMS were not significantly different from those measured by the external standard method. Conclusion. QAMS can be used to determine the content of 4 components in Plantago depressa at the same time, and the method is simple, accurate, and can be used for quality control.

Simultaneous Determination of Five Active Components in the Chinese Patent Medicine Niuhuang Jiangya Pill by HPLC-MS/MS

2017 ◽  
Vol 100 (3) ◽  
pp. 717-720
Author(s):  
Shan Xiong ◽  
Shanshan Lei
Keyword(s):  
Simultaneous Determination ◽  
Quality Evaluation ◽  
Gradient Elution ◽  
Astragaloside Iv ◽  
Active Components ◽  
Patent Medicines ◽  
Chinese Patent Medicine ◽  
Chinese Patent

Abstract Niuhuang Jiangya (NHJY) pill is one of the well-known Chinese patent medicines in China used in the treatment of high blood pressure. The primary purpose of this study was to establish and validate a method using HPLC with tandem MS for the quality evaluation of NHJY pill through simultaneous determination of the following five active components: baicalin, paeoniflorin, astragaloside IV, ferulic acid, and emodin. Chromatographic separation was carried out on a Hypersil GOLD HPLC C18 column (50 × 4.6 mm, 3 μm) with acetonitrileand water as mobile phase and gradient elution at a flow rateof 0.4 mL/min. The method established in this study was selective, linear, precise, and accurate and was successfully applied to evaluate five active components in NHJY pill collectedfrom different production batches, which could be considered a good approach to control the quality of NHJY pill and other related botanical drugs.

scholarly journals Variations in Physiology and Multiple Bioactive Constituents under Salt Stress Provide Insight into the Quality Evaluation of Apocyni Veneti Folium

2018 ◽  
Vol 19 (10) ◽  
pp. 3042 ◽  
Author(s):  
Cuihua Chen ◽  
Chengcheng Wang ◽  
Zixiu Liu ◽  
Xunhong Liu ◽  
Lisi Zou ◽  
...  
Keyword(s):  
Salt Stress ◽  
Statistical Analysis ◽  
Organic Acids ◽  
Multivariate Statistical Analysis ◽  
Quality Evaluation ◽  
Gray Relational Analysis ◽  
Lipid Peroxidation Product ◽  
Bioactive Constituents ◽  
Multivariate Statistical

As one of the major abiotic stresses, salinity stress may affect the physiology and biochemical components of Apocynum venetum L. To systematically evaluate the quality of Apocyni Veneti Folium (AVF) from the perspective of physiological and the wide variety of bioactive components response to various concentrations of salt stress, this experiment was arranged on the basis of ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) technology and multivariate statistical analysis. Physiological characteristics of photosynthetic pigments, osmotic homeostasis, lipid peroxidation product, and antioxidative enzymes were introduced to investigate the salt tolerance mechanism of AVF under salinity treatments of four concentrations (0, 100, 200, and 300 mM NaCl, respectively). Furthermore, a total of 43 bioactive constituents, including 14 amino acids, nine nucleosides, six organic acids, and 14 flavonoids were quantified in AVF under salt stress. In addition, multivariate statistical analysis, including hierarchical clustering analysis, principal component analysis (PCA), and gray relational analysis (GRA) was employed to systematically cluster, distinguish, and evaluate the samples, respectively. Compared with the control, the results demonstrated that 200 mM and 100 mM salt stress contributed to maintain high quality of photosynthesis, osmotic balance, antioxidant enzyme activity, and the accumulation of metabolites, except for total organic acids, and the quality of AVF obtained by these two groups was better than others; however, under severe stress, the accumulation of the oxidative damage and the reduction of metabolite caused by inefficiently scavenging reactive oxygen species (ROS) lead to lower quality. In summary, the proposed method may provide integrated information for the quality evaluation of AVF and other salt-tolerant Chinese medicines.

INFLUENCE OF DICARBOXYLIC ACIDS ON SOWING QUALITY OF SPRING BARLEY

1970 ◽  
pp. 22-25
Author(s):  
N. N. Loy ◽  
S. N. Gulina
Keyword(s):  
Organic Acids ◽  
Spring Barley ◽  
Dose Range ◽  
Dicarboxylic Acids ◽  
Rotary Machine ◽  
Working Solution ◽  
Negative Effect ◽  
Sprouted Seeds

The effect of presowing seed treatment on various concentrations of dicarboxylic (organic) acids on the sowing characteristics of spring barley has been studied. Seeds were treated with organic acids obtained by exposing cuttings to the radiation with a dose of 100 kGy and consequent hydrolysis, in concentrations: 1•10-7 %; 1•10-9; 1•10-11; 1•10-13 and 1•10-15 % on a laboratory rotary machine RVO-64 for one day before laying for germination. Distilled water was used for the control case. The rate of application of the working solution calculated as 10 liters / ton of seeds. Seeds were germinated in filter paper rolls in accordance with GOST 12038-84 requirements. The temperature was maintained at +24 ° C in the thermostat where the glasses with rolls were placed. For determination of germinative power and laboratory germination the sprouted seeds were evaluated after three and seven days, respectively. In laboratory experiments it was established that the treatment of barley seeds of varieties Zazersky 85, Nur and Vladimir with organic acids (OK) in different concentrations had both a stimulating and a negative effect. On the Zazersky 85 variety, in variants with acid concentrations of 1•10-9 and 1•10-11, an increase in germination energy (EP) by 2-4% and a significant decrease (by 3-4%) of laboratory germination (LV) of barley seeds were noted. On the Nur variety, the increase in EP was observed at 4% (concentration 1•10-11), LV and seed growth force (CPC) by 2-7% at a concentration of 1•10-7 and in the dose range 1•10-11 - 1•10-14 compared to the control values. On the grade of Vladimir, an increase in EP, LV, and CPC was found to increase by 1-6% at concentrations OK 1•10-7 and 1•10-13. It was shown that the treatment of seeds with acids led to an increase in the length of the germ in all studied varieties (by 3-9%) and dry biomass of 7-day-old seedlings - by 3-6%. Consequently, the treatment of seeds with a mixture of dicarboxylic acids has a stimulating effect on the sowing quality of spring barley.

Improved LC Method for the Simultaneous Determination of Five Active Components in Danshen and Its Preparations

2009 ◽  
Vol 69 (5-6) ◽  
pp. 543-548 ◽  
Author(s):  
Xiao-Bing Li ◽  
Xiao-Mei Xie ◽  
Wei-Zhong Pei ◽  
Jia-Kuan Chen ◽  
Yan Song ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Active Components
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