Naked-eye sensitive detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) based on a horseradish peroxidase catalytic colorimetric system with Cu(ii)

The Analyst ◽  
2016 ◽  
Vol 141 (19) ◽  
pp. 5549-5554 ◽  
Author(s):  
Dongmin Shi ◽  
Yue Sun ◽  
Lin Lin ◽  
Chunjun Shi ◽  
Guangfeng Wang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
Colorimetric Method ◽  
Sensitive Detection ◽  
Naked Eye ◽  
Colorimetric System ◽  
System P

In this paper, a novel colorimetric method for the detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) was designed based on a Cu2+–horseradish peroxidase (HRP)–3,3′,5,5′-tetra-methylbenzidine (TMB)–H2O2 system.

Highly sensitive ‘naked-eye’ colorimetric detection of thiourea using gold nanoparticles

2015 ◽  
Vol 7 (12) ◽  
pp. 4927-4933 ◽  
Author(s):  
Yi-Liang Cao ◽  
Yan Li ◽  
Fei Zhang ◽  
Jian-Zhong Huo ◽  
Xiao-Jun Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Naked Eye ◽  
Colorimetric System ◽  
Highly Sensitive ◽  
Aqueous Environments

A non aggregation colorimetric system was developed for the fast and sensitive detection of thiourea in aqueous environments using an unlabeled gold nanoparticle probe.

Naked-eye colorimetric detection of HCV RNA mediated by a 5′ UTR-targeted antisense oligonucleotide and plasmonic gold nanoparticles

The Analyst ◽  
2021 ◽  
Author(s):  
Almas Shamaila Mohammed ◽  
Aniket Balapure ◽  
Mahammad Nanne Khaja ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta
Keyword(s):  
Gold Nanoparticles ◽  
Antisense Oligonucleotide ◽  
Early Stage ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Clinical Samples ◽  
Hcv Rna ◽  
Naked Eye

An Au NP based facile strategy for the rapid, early-stage, and sensitive detection of HCV RNA in clinical samples which avoids thiol tagging to the antisense oligonucleotide and expensive infrastructure is presented.

A Simple Colorimetric Method for Naked-Eye Detection of Circulating Cell-Free DNA Using Unlabelled Gold Nanoparticles

2018 ◽  
Vol 3 (41) ◽  
pp. 11541-11551 ◽  
Author(s):  
Shweta Khanna ◽  
Prasanta Padhan ◽  
Sourav Das ◽  
Kumar Sagar Jaiswal ◽  
Archana Tripathy ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Eye Detection ◽  
Cell Free Dna ◽  
Naked Eye ◽  
Free Dna ◽  
Naked Eye Detection ◽  
Circulating Cell Free Dna

scholarly journals New chromogens for alkaline phosphatase histochemistry: salmon and magenta phosphate are useful for single- and double-label immunohistochemistry.

1994 ◽  
Vol 42 (4) ◽  
pp. 551-554 ◽  
Author(s):  
C Avivi ◽  
O Rosen ◽  
R S Goldstein
Keyword(s):  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
Molecular Biology ◽  
Horseradish Peroxidase ◽  
Reaction Products ◽  
Immunocytochemical Detection ◽  
Hematoxylin Staining ◽  
Double Label ◽  
Biology Research

Two new substrate chromogens for alkaline phosphatase (ALP) detection have been recently synthesized for use in molecular biology research, salmon and magenta phosphate. We show here that these two chromogens have advantageous characteristics for immunocytochemistry as well. Their relatively delicate pink- and magenta-colored products do not mask the colors produced by other staining procedures. In addition, the reaction products of these substrates are insoluble in water, ethanol, and xylene, permitting the use of regressive hematoxylin staining procedures and coverslipping in permanent resin-based media. Most importantly, when these ALP substrates are used in double-label immunocytochemistry in combination with horseradish peroxidase-diaminobenzidine (HRP-DAB) and counterstained with hematoxylin, all three colors can be easily distinguished. An application using these substrates for simultaneous immunocytochemical detection of two monoclonal antibodies of different classes, in combination with hematoxylin staining, is illustrated.

scholarly journals One-step double immunolabeling of mouse interdigitating reticular cells: simultaneous application of pre-formed complexes of monoclonal rat antibody M1-8 with horseradish peroxidase-linked anti-rat immunoglobulins and of monoclonal mouse anti-Ia antibody with alkaline phosphatase-coupled anti-mouse immunoglobulins.

1991 ◽  
Vol 39 (12) ◽  
pp. 1719-1723 ◽  
Author(s):  
T Krenács ◽  
H Uda ◽  
S Tanaka
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
B Lymphocytes ◽  
Enzyme Reaction ◽  
Molecular Complexes ◽  
Simultaneous Application ◽  
One Step ◽  
Reticular Cells ◽  
Secondary Antibodies ◽  
Double Immunolabeling

A novel one-step double immunolabeling method was elaborated on the basis of the simultaneous application of preformed molecular complexes of two primary antibodies with their specific secondary antibodies labeled with different enzymes. Treatment with a rat monoclonal antibody (MAb), M1-8, pre-coupled with horseradish peroxidase-linked sheep anti-rat immunoglobulins, and enzyme reaction revealed by the 3-amino-9-ethylcarbazole/hydrogen peroxide reaction, resulted in red-brown intracytoplasmic staining of interdigitating reticular cells in the lymph nodes of Balb/c mice. Another molecular complex, made of mouse anti-Ia MAb with alkaline phosphatase-linked rabbit anti-mouse immunoglobulins, applied at the same time and then developed with naphthol AS-BI-phosphate/fast blue BB as substrate, yielded blue surface staining of this cell type in addition to labeling of B-lymphocytes. The method described provides the possibility of relatively rapid double antigen detection where the binding sites of the secondary antibodies are saturated by the specific primary immunoglobulins. This approach seems to avoid nonspecific binding of primary antibodies to Fc receptors, and the unwanted binding of secondary antibodies with cell surface immunoglobulins on B-lymphocytes or with crossreactive primary antibodies used in the other sequence, if the primary antibodies and the tissue are the same or crossreactive animal species.

scholarly journals A Facile Colorimetric Method For Ultra-Rapid And Sensitive Detection of Copper Ions In Water

2021 ◽  
Author(s):  
Lei Chen ◽  
Yan Li ◽  
Ping Sun ◽  
Hualin Chen ◽  
He Li ◽  
...  
Keyword(s):  
Color Change ◽  
Copper Ions ◽  
Colorimetric Method ◽  
Ethanol Solution ◽  
Sensitive Detection ◽  
World Health ◽  
Great Promise ◽  
Experimental Conditions ◽  
Interesting Phenomenon ◽  
Health Organization

Abstract It is of great meaning to develop a facile, reliable and sensitive method to detect copper ions in water. In the study, a facile method has been developed for rapid and sensitive detection of Cu2+. An interesting phenomenon has been observed that 3,3',5,5'-tetramethylbenzidine (TMB) ethanol solution can be extremely fast passed from colorless to yellow once Cu2+ ions are added. It easily occurs to us that Cu2+ can be quantitatively determined via the absorbance at 904 nm of the color changed TMB solution. More importantly, some specific anions (Cl- , Br- ) can significantly enhance the absorption intensity. Under the optimized experimental conditions, this method exhibits a good linear response range for Cu2+ from 0.5 to 100 μM, with the detection limit of 93 nM. Moreover, the possible detection principle has been explored. It is worth mentioning that the color change can be clearly observed by naked eyes for the detection of 1 μM Cu2+, which is far below the threshold limit of Cu2+ in drinking water suggested by World Health Organization. It means that this method possess great promise for on-site Cu2+ detection.

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