Size‐dependent light scattering of CoOOH nanoflakes for convenient and sensitive detection of alkaline phosphatase in human serum

Luminescence ◽  
2021 ◽  
Author(s):  
Lu Ning Zhu ◽  
Ru Cheng ◽  
Kai Wen Kang ◽  
Ming Yun Chen ◽  
Tianrong Zhan ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Light Scattering ◽  
Human Serum ◽  
Sensitive Detection ◽  
Size Dependent ◽  
Coooh Nanoflakes

Size-dependent modulation of CoOOH nanoflakes light scattering for rapid and selective detection of tetracycline in milk

2018 ◽  
Vol 2 (4) ◽  
pp. 332-341 ◽  
Author(s):  
Yin Hui Wei ◽  
Xin Yu Li ◽  
Jie Gao ◽  
Jia Jun Liu ◽  
Dan Yuan ◽  
...  
Keyword(s):  
Light Scattering ◽  
Selective Detection ◽  
Size Dependent ◽  
Coooh Nanoflakes

A CoOOH nanoflake-based light scattering probe for the simple and selective detection of uric acid in human serum

2018 ◽  
Vol 10 (40) ◽  
pp. 4951-4957 ◽  
Author(s):  
Zhi Yuan Mao ◽  
Lu Ning Zhu ◽  
Jie Gao ◽  
Jia Jun Liu ◽  
Yin Hui Wei ◽  
...  
Keyword(s):  
Uric Acid ◽  
Light Scattering ◽  
Human Serum ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Simple Strategy ◽  
Lower Light ◽  
Coooh Nanoflakes ◽  
Reduced Light ◽  
The Relationship

In the presence of uricase, uric acid generated H2O2, which further decomposed CoOOH nanoflakes to release Co2+, resulting in smaller nanoparticles with lower light scattering. Based on the relationship between the reduced light scattering and uric acid concentration, the simple strategy was applicable to uric acid sensing in human serum samples.

scholarly journals An Exonuclease I-Aided Turn-Off Fluorescent Strategy for Alkaline Phosphatase Assay Based on Terminal Protection and Copper Nanoparticles

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 139
Author(s):  
Yan Wang ◽  
Ying Yan ◽  
Xinfa Liu ◽  
Changbei Ma
Keyword(s):  
Alkaline Phosphatase ◽  
Human Serum ◽  
Clinical Diagnosis ◽  
Copper Nanoparticles ◽  
Cost Effective ◽  
Fast Method ◽  
Exonuclease I ◽  
Alkaline Phosphatase Assay ◽  
Phosphatase Assay ◽  
Phosphatase Alkaline

As an important DNA 3′-phosphatase, alkaline phosphatase can repair damaged DNA caused by replication and recombination. It is essential to measure the level of alkaline phosphatase to indicate some potential diseases, such as cancer, related to alkaline phosphatase. Here, we designed a simple and fast method to detect alkaline phosphatase quantitively. When alkaline phosphatase is present, the resulting poly T-DNA with a 3′-hydroxyl end was cleaved by exonuclease I, prohibiting the formation of fluorescent copper nanoparticles. However, the fluorescent copper nanoparticles can be monitored with the absence of alkaline phosphatase. Hence, we can detect alkaline phosphatase with this turn-off strategy. The proposed method is able to quantify the concentration of alkaline phosphatase with the LOD of 0.0098 U/L. Furthermore, we utilized this method to measure the effects of inhibitor Na3VO4 on alkaline phosphatase. In addition, it was successfully applied to quantify the level of alkaline phosphatase in human serum. The proposed strategy is sensitive, selective, cost effective, and timesaving, having a great potential to detect alkaline phosphatase quantitatively in clinical diagnosis.

scholarly journals Selective enrichment and sensitive detection of peptide and proteinbiomarkers in human serum using polymeric reverse micelles and MALDI-MS

The Analyst ◽  
2012 ◽  
Vol 137 (4) ◽  
pp. 1024-1030 ◽  
Author(s):  
Nadnudda Rodthongkum ◽  
Rajasekhar Ramireddy ◽  
S. Thayumanavan ◽  
W. Vachet Richard
Keyword(s):  
Human Serum ◽  
Reverse Micelles ◽  
Maldi Ms ◽  
Sensitive Detection ◽  
Selective Enrichment
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