A fluorescent turn-on probe for visualizing lysosomes in hypoxic tumor cells

The Analyst ◽  
2016 ◽  
Vol 141 (10) ◽  
pp. 2879-2882 ◽  
Author(s):  
Sihang Luo ◽  
Yingchao Liu ◽  
Feiyi Wang ◽  
Qiang Fei ◽  
Ben Shi ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Fluorescent Probe ◽  
Reductive Cleavage ◽  
Strong Fluorescence ◽  
Turn On ◽  
Hypoxic Tumor

A fluorescent probe localized in lysosomes after reductive cleavage with azoreductase gave strong fluorescence, providing specificity for imaging lysosomes in hypoxic cancer cells.

A cyanine-derived “turn-on” fluorescent probe for imaging nitroreductase in hypoxic tumor cells

2015 ◽  
Vol 7 (24) ◽  
pp. 10125-10128 ◽  
Author(s):  
Cong Xue ◽  
Yingjie Lei ◽  
Sichun Zhang ◽  
Yaowu Sha
Keyword(s):  
Tumor Cells ◽  
Fluorescent Probe ◽  
Phenol Group ◽  
Turn On ◽  
Hypoxic Tumor

A new “turn-on” fluorescent probe, composed of a protected phenol group with ap-nitrobenzyl moiety that functions as a latent donor and conjugated with two benzo[f]indolinium acceptors, was developed and applied for imaging nitroreductase (NTR) in hypoxic tumor cells.

A rapid response “Turn-On” fluorescent probe for nitroreductase detection and its application in hypoxic tumor cell imaging

The Analyst ◽  
2015 ◽  
Vol 140 (2) ◽  
pp. 574-581 ◽  
Author(s):  
Jian Xu ◽  
Shaobo Sun ◽  
Qian Li ◽  
Ying Yue ◽  
Yingdong Li ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Fluorescent Probe ◽  
Cell Imaging ◽  
Rapid Response ◽  
Indirect Detection ◽  
Turn On ◽  
Hypoxic Tumor

A novel “Turn-On” fluorescent probe, quaternarized 4-pyridinyl-substituted BODIPY dye by incorporating a 5-nitrofuran moiety, was developed and applied for imaging the hypoxic status of tumor cells by the indirect detection of nitroreductase.

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

scholarly journals Aneuploid Circulating Tumor-Derived Endothelial Cell (CTEC): A Novel Versatile Player in Tumor Neovascularization and Cancer Metastasis

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1539 ◽  
Author(s):  
Peter Ping Lin
Keyword(s):  
Endothelial Cells ◽  
Tumor Microenvironment ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Cancer Metastasis ◽  
Epithelial To Mesenchymal Transition ◽  
Vasculogenic Mimicry ◽  
Mesenchymal Transition ◽  
Tumor Neovascularization ◽  
Hypoxic Tumor

Hematogenous and lymphogenous cancer metastases are significantly impacted by tumor neovascularization, which predominantly consists of blood vessel-relevant angiogenesis, vasculogenesis, vasculogenic mimicry, and lymphatic vessel-related lymphangiogenesis. Among the endothelial cells that make up the lining of tumor vasculature, a majority of them are tumor-derived endothelial cells (TECs), exhibiting cytogenetic abnormalities of aneuploid chromosomes. Aneuploid TECs are generated from “cancerization of stromal endothelial cells” and “endothelialization of carcinoma cells” in the hypoxic tumor microenvironment. Both processes crucially engage the hypoxia-triggered epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndoMT). Compared to the cancerization process, endothelialization of cancer cells, which comprises the fusion of tumor cells with endothelial cells and transdifferentiation of cancer cells into TECs, is the dominant pathway. Tumor-derived endothelial cells, possessing the dual properties of cancerous malignancy and endothelial vascularization ability, are thus the endothelialized cancer cells. Circulating tumor-derived endothelial cells (CTECs) are TECs shed into the peripheral circulation. Aneuploid CD31+ CTECs, together with their counterpart CD31- circulating tumor cells (CTCs), constitute a unique pair of cellular circulating tumor biomarkers. This review discusses a proposed cascaded framework that focuses on the origins of TECs and CTECs in the hypoxic tumor microenvironment and their clinical implications for tumorigenesis, neovascularization, disease progression, and cancer metastasis. Aneuploid CTECs, harboring hybridized properties of malignancy, vascularization and motility, may serve as a unique target for developing a novel metastasis blockade cancer therapy.

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

scholarly journals A universal fluorogenic switch for Fe(ii) ion based on N-oxide chemistry permits the visualization of intracellular redox equilibrium shift towards labile iron in hypoxic tumor cells

2017 ◽  
Vol 8 (7) ◽  
pp. 4858-4866 ◽  
Author(s):  
Tasuku Hirayama ◽  
Hitomi Tsuboi ◽  
Masato Niwa ◽  
Ayaji Miki ◽  
Satoki Kadota ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescent Probe ◽  
Redox Equilibrium ◽  
Hypoxic Tumor ◽  
Equilibrium Shift ◽  
Labile Iron ◽  
Intracellular Redox

Oxygen-dependent fluctuation of labile Fe(ii) was visualized by a new N-oxide-based fluorescent probe for Fe(ii) ion.

A NIR turn-on fluorescent probe applied in cytochrome P450 reductase detection and hypoxia imaging in tumor cells

2017 ◽  
Vol 139 ◽  
pp. 587-592 ◽  
Author(s):  
Lei Cui ◽  
Yanping Shi ◽  
Shuping Zhang ◽  
Luliang Yan ◽  
Huan Zhang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Tumor Cells ◽  
Fluorescent Probe ◽  
Cytochrome P450 Reductase ◽  
Hypoxia Imaging ◽  
Turn On ◽  
P450 Reductase

A highly selective turn-on fluorescent probe based on semi-cyanine for the detection of nitroreductase and hypoxic tumor cell imaging

RSC Advances ◽  
2014 ◽  
Vol 4 (99) ◽  
pp. 56207-56210 ◽  
Author(s):  
Jun Yuan ◽  
Yu-Qiong Xu ◽  
Nan-Nan Zhou ◽  
Rui Wang ◽  
Xu-Hong Qian ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Fluorescent Probe ◽  
Cell Imaging ◽  
Turn On ◽  
Hypoxic Tumor

A selective turn-on fluorescent probe based on semi-cyanine for the detection of nitroreductase (NTR) and hypoxia was designed and synthesized.

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