scholarly journals In vitro toxicological characterisation of arsenic-containing fatty acids and three of their metabolites

2015 ◽  
Vol 4 (5) ◽  
pp. 1289-1296 ◽  
Author(s):  
S. Meyer ◽  
G. Raber ◽  
F. Ebert ◽  
L. Leffers ◽  
S. M. Müller ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Human Liver ◽  
Liver Cells ◽  
Human Liver Cells ◽  
Cells In Culture

Arsenic-containing fatty acids are bioavailable and toxic to human liver cells in culture.

AAnti-Inflammatory Effects of Plasma Circulating Exosomes Obtained from Normal-Weight and Obese Subjects on Hepatocytes

2020 ◽  
Vol 20 ◽  
Author(s):  
Reza Afrisham ◽  
Sahar Sadegh-Nejadi ◽  
Reza Meshkani ◽  
Solaleh Emamgholipour ◽  
Molood Bagherieh ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Liver Cells ◽  
Normal Weight ◽  
Protein Levels ◽  
Human Liver Cells ◽  
Tnf Α ◽  
Anti Inflammatory

Introduction: Obesity is a disorder with low-grade chronic inflammation that plays a key role in the hepatic inflammation and steatosis. Moreover, there are studies to support the role of exosomes in the cellular communications, the regulation of metabolic homeostasis and immunomodulatory activity. Accordingly, we aimed to evaluate the influence of plasma circulating exosomes derived from females with normal-weight and obesity on the secretion of inflammatory cytokines in human liver cells. Methods: Plasma circulating exosomes were isolated from four normal (N-Exo) and four obese (O-Exo) women. The exosomes were characterized and approved for CD63 expression (common exosomal protein marker) and morphology/size using the western blot and TEM methods, respectively. The exosomes were used for stimulation of HepG2 cells in vitro. After 24 h incubation, the protein levels of TNF-α,IL-6, and IL-1β were measured in the culture supernatant of HepG2 cells using the ELISA kit. Results: The protein levels of IL-6 and TNF-α in the cells treated with O-Exo and N-Exo reduced significantly in comparison with control group (P=0.039 and P<0.001 respectively), while significance differences were not found between normal and obese groups (P=0.808, and P=0.978 respectively). However, no significant differences were found between three groups in term of IL-1β levels (P=0.069). Based on the correlation analysis, the protein levels of IL-6 were positively correlated with TNF-α (r 0.978, P<0.001). Conclusion: These findings suggest that plasma circulating exosomes have probably anti-inflammatory properties independently from body mass index and may decrease the secretion of inflammatory cytokines in liver. However, further investigations in vitro and in vivo are needed to address the anti-inflammatory function of N-Exo and O-Exo in human liver cells and/or other cells.

Comparative hepatotoxicity of deoxynivalenol in rat, mouse and human liver cells in culture

2010 ◽  
Vol 30 (6) ◽  
pp. 566-573 ◽  
Author(s):  
Saura C. Sahu ◽  
Michael W. O'Donnell ◽  
Paddy L. Wiesenfeld
Keyword(s):  
Human Liver ◽  
Liver Cells ◽  
Human Liver Cells ◽  
Cells In Culture

The effect of liver donors’ age, gender and metabolic state on pancreatic lineage activation

2021 ◽  
Vol 16 (1) ◽  
pp. 19-31
Author(s):  
Ioan V Matei ◽  
Irit Meivar-Levy ◽  
Daniela Lixandru ◽  
Simona Dima ◽  
Ioana R Florea ◽  
...  
Keyword(s):  
Replacement Therapy ◽  
Human Liver ◽  
Liver Cells ◽  
Diabetic Patients ◽  
Metabolic State ◽  
Human Liver Cells ◽  
Metabolic States ◽  
Different Ages ◽  
Liver Donors

Autologous cells replacement therapy by liver to pancreas transdifferentiation (TD) allows diabetic patients to be also the donors of their own therapeutic tissue. Aim: To analyze whether the efficiency of the process is affected by liver donors’ heterogeneity with regard to age, gender and the metabolic state. Materials & methods: TD of liver cells derived from nondiabetic and diabetic donors at different ages was characterized at molecular and cellular levels, in vitro. Results: Neither liver cells proliferation nor the propagated cells TD efficiency directly correlate with the age (3–60 years), gender or the metabolic state of the donors. Conclusion: Human liver cells derived from a wide array of ages and metabolic states can be used for autologous cells therapies for diabetics.

Protective effects of papaya extracts on tert-butyl hydroperoxide mediated oxidative injury to human liver cells (An in-vitro study)

2012 ◽  
Vol 2 (3) ◽  
pp. 10-19 ◽  
Author(s):  
Sheri-Ann Tan ◽  
Sonia Ramos ◽  
María Angeles Martin ◽  
Raquel Mateos ◽  
Michael Harvey ◽  
...  
Keyword(s):  
Human Liver ◽  
In Vitro Study ◽  
Oxidative Injury ◽  
Liver Cells ◽  
Vitro Study ◽  
Protective Effects ◽  
Butyl Hydroperoxide ◽  
Human Liver Cells ◽  
Tert Butyl Hydroperoxide

scholarly journals Improvement of therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet

2020 ◽  
Author(s):  
Yu Na Lee ◽  
Hye-Jin Yi ◽  
Eun Hye Seo ◽  
Jooyun Oh ◽  
Song Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Human Liver ◽  
Cell Sheet ◽  
Liver Cells ◽  
Cell Sheets ◽  
Human Liver Cells ◽  
Insulin Producing Cells ◽  
Differentiation Efficiency

Abstract Background: Although pancreatic islet transplantation therapy is ideal for diabetes patients, several hurdles have prevented it from becoming a standard treatment, including donor shortage and low engraftment efficacy. In this study, we prepared insulin-producing cells trans-differentiated from adult human liver cells as a new islet source. Also, cell sheets formation could improve differentiation efficiency and graft survival.Methods: Liver cells were expanded in vitro and trans-differentiated to IPCs using adenovirus vectors carrying human genes for PDX1, NEUROD1 and MAFA. IPCs were seeded on temperature-responsive culture dishes to form cell sheets. Differentiation efficiency were confirmed by ß cell-specific gene expression, insulin production, and immunohistochemistry. IPCs suspension was injected by portal vein (PV), and IPCs sheet was transplanted on the liver surface of the diabetic nude mouse. The therapeutic effect of IPC sheet was evaluated by comparing blood glucose control, weight gain, histological evaluation and hepatotoxicity with IPCs injection group. Also, cell biodistribution was assessed by in vivo/ex vivo fluorescence image tagging.Results: Insulin gene expression and protein production were significantly increased on IPC sheets compared with those in IPCs cultured on conventional culture dishes. Transplanted IPC sheets displayed significantly higher engraftment efficiency and fewer transplanted cells in other organs than injected IPCs, and also lower liver toxicity, improved blood glucose levels, and weight gain. One and two weeks following IPC sheet transplantation, immunohistochemical analyses of liver tissue revealed positive staining for PDX1 and insulin.Conclusions: In conclusion, cell sheet formation enhanced the differentiation function and maturation of IPCs in vitro. Additionally, parameters for clinical application such as distribution, therapeutic efficacy, and toxicity were favorable. The cell sheet technique may be used with IPCs derived from various cell sources in clinical applications.

Cytotoxicity evaluation of the alkaloid govaniadinein human liver cells and in vitro glucuronidation by liver microsomes

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LMM Marques ◽  
U Rottkord ◽  
I Krug ◽  
M Behrens ◽  
A Adhikari ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Microsomes ◽  
Liver Cells ◽  
Human Liver Cells
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