Protective effects of papaya extracts on tert-butyl hydroperoxide mediated oxidative injury to human liver cells (An in-vitro study)

2012 ◽  
Vol 2 (3) ◽  
pp. 10-19 ◽  
Author(s):  
Sheri-Ann Tan ◽  
Sonia Ramos ◽  
María Angeles Martin ◽  
Raquel Mateos ◽  
Michael Harvey ◽  
...  
Keyword(s):  
Human Liver ◽  
In Vitro Study ◽  
Oxidative Injury ◽  
Liver Cells ◽  
Vitro Study ◽  
Protective Effects ◽  
Butyl Hydroperoxide ◽  
Human Liver Cells ◽  
Tert Butyl Hydroperoxide

scholarly journals In vitro investigations of Cynara scolymus L. extract on cell physiology of HepG2 liver cells

2009 ◽  
Vol 45 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Gesine Löhr ◽  
Alexandra Deters ◽  
Andreas Hensel
Keyword(s):  
Gene Expression ◽  
Human Liver ◽  
Leaf Extract ◽  
Liver Cells ◽  
Mitochondrial Activity ◽  
Cell Physiology ◽  
Protective Effects ◽  
Human Liver Cells ◽  
Artichoke Leaf Extract

The objective of this study was the investigation of a potential influence of artichoke leaf extract (ALE) on the cell physiology and gene expression of phase I/II enzymes of human liver cells HepG2 and investigation on potential cell protective effects against ethanol-induced cell toxicity against HepG2 cells. Cell biological assays under in vitro conditions using HepG2 liver cells and investigation of mitochondrial activity (MTT test), proliferation assay (BrdU incorporation ELISA), LDH as toxicity marker, gene expression analysis by RT-PCR and enzyme activity of glutationtransferase. Artichocke extract, containing 27% caffeoylquinic acids and 7% flavonoids induced mitochondrial activity, proliferation and total protein content under in vitro conditions in human liver cells HepG2. These effects could not be correlated to the well-known artichoke secondary compounds cynarin, caffeic acid, chlorogenic acid, luteolin and luteolin-7-O-glucoside. The flavones luteolin and luteolin-7-O-glucoside had inhibitory effects at 100 µg/mL level on HepG2 cells, with luteolin being a significant stronger inhibitor compared to the respective glucoside. Artichoke leaf extract had minor stimulating effect on gene expression of CYP1A2, while CYP3A4, GGT, GPX2, GSR and GST were slightly inhibited. GST inhibition under in vitro conditions was also shown by quantification of GST enzyme activity. Induction of gene expression of CYP1A2 was shown to be supraadditive after simultaneous application of ethanol plus artichoke extract. Artichoke leaf extract exhibited cell protective effects against ethanol-induced toxicity within cotreatment under in vitro conditions. Also H2O2 damage was significantly inhibited by simultaneous artichoke incubation. Pre- and posttreatments did not exert protective effects. DMSO-induced toxicity was significantly reduced by pre-, post- and cotreatment with artichoke extract and especially with luteolin-7-O-glucoside, indicating a direct interaction with the toxifying agent and an induction of repair mechanisms.

In Vitro Study of Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX) Metabolism in Human Liver

2008 ◽  
Author(s):  
Cheng J. Cao ◽  
Gunda Reddy ◽  
Desmond I. Bannon ◽  
Mark S. Johnson
Keyword(s):  
Human Liver ◽  
In Vitro Study ◽  
Vitro Study

AAnti-Inflammatory Effects of Plasma Circulating Exosomes Obtained from Normal-Weight and Obese Subjects on Hepatocytes

2020 ◽  
Vol 20 ◽  
Author(s):  
Reza Afrisham ◽  
Sahar Sadegh-Nejadi ◽  
Reza Meshkani ◽  
Solaleh Emamgholipour ◽  
Molood Bagherieh ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Liver Cells ◽  
Normal Weight ◽  
Protein Levels ◽  
Human Liver Cells ◽  
Tnf Α ◽  
Anti Inflammatory

Introduction: Obesity is a disorder with low-grade chronic inflammation that plays a key role in the hepatic inflammation and steatosis. Moreover, there are studies to support the role of exosomes in the cellular communications, the regulation of metabolic homeostasis and immunomodulatory activity. Accordingly, we aimed to evaluate the influence of plasma circulating exosomes derived from females with normal-weight and obesity on the secretion of inflammatory cytokines in human liver cells. Methods: Plasma circulating exosomes were isolated from four normal (N-Exo) and four obese (O-Exo) women. The exosomes were characterized and approved for CD63 expression (common exosomal protein marker) and morphology/size using the western blot and TEM methods, respectively. The exosomes were used for stimulation of HepG2 cells in vitro. After 24 h incubation, the protein levels of TNF-α,IL-6, and IL-1β were measured in the culture supernatant of HepG2 cells using the ELISA kit. Results: The protein levels of IL-6 and TNF-α in the cells treated with O-Exo and N-Exo reduced significantly in comparison with control group (P=0.039 and P<0.001 respectively), while significance differences were not found between normal and obese groups (P=0.808, and P=0.978 respectively). However, no significant differences were found between three groups in term of IL-1β levels (P=0.069). Based on the correlation analysis, the protein levels of IL-6 were positively correlated with TNF-α (r 0.978, P<0.001). Conclusion: These findings suggest that plasma circulating exosomes have probably anti-inflammatory properties independently from body mass index and may decrease the secretion of inflammatory cytokines in liver. However, further investigations in vitro and in vivo are needed to address the anti-inflammatory function of N-Exo and O-Exo in human liver cells and/or other cells.

Long lasting protective effects of early l-arginine treatment on endothelium in an in vitro study

2021 ◽  
Vol 40 (4) ◽  
pp. 1519-1529
Author(s):  
Elena Galluccio ◽  
Serena Spadoni ◽  
Barbara Fontana ◽  
Emanuele Bosi ◽  
Piermarco Piatti ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Protective Effects

The effect of liver donors’ age, gender and metabolic state on pancreatic lineage activation

2021 ◽  
Vol 16 (1) ◽  
pp. 19-31
Author(s):  
Ioan V Matei ◽  
Irit Meivar-Levy ◽  
Daniela Lixandru ◽  
Simona Dima ◽  
Ioana R Florea ◽  
...  
Keyword(s):  
Replacement Therapy ◽  
Human Liver ◽  
Liver Cells ◽  
Diabetic Patients ◽  
Metabolic State ◽  
Human Liver Cells ◽  
Metabolic States ◽  
Different Ages ◽  
Liver Donors

Autologous cells replacement therapy by liver to pancreas transdifferentiation (TD) allows diabetic patients to be also the donors of their own therapeutic tissue. Aim: To analyze whether the efficiency of the process is affected by liver donors’ heterogeneity with regard to age, gender and the metabolic state. Materials & methods: TD of liver cells derived from nondiabetic and diabetic donors at different ages was characterized at molecular and cellular levels, in vitro. Results: Neither liver cells proliferation nor the propagated cells TD efficiency directly correlate with the age (3–60 years), gender or the metabolic state of the donors. Conclusion: Human liver cells derived from a wide array of ages and metabolic states can be used for autologous cells therapies for diabetics.

The influence of amitriptyline and carbamazepine on levomepromazine metabolism in human liver: An in vitro study

2014 ◽  
Vol 66 (6) ◽  
pp. 1122-1126 ◽  
Author(s):  
Jacek Wójcikowski ◽  
Agnieszka Basińska ◽  
Jan Boksa ◽  
Władysława A. Daniel
Keyword(s):  
Human Liver ◽  
In Vitro Study ◽  
Vitro Study

In vitro study on the biotransformation and cytotoxicity of three hexabromocyclododecane diastereoisomers in liver cells

Chemosphere ◽  
2016 ◽  
Vol 161 ◽  
pp. 251-258 ◽  
Author(s):  
Xiaomei Huang ◽  
Cen Chen ◽  
Yu Shang ◽  
Yufang Zhong ◽  
Guofa Ren ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Liver Cells ◽  
Vitro Study

scholarly journals Improvement of therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet

2020 ◽  
Author(s):  
Yu Na Lee ◽  
Hye-Jin Yi ◽  
Eun Hye Seo ◽  
Jooyun Oh ◽  
Song Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Human Liver ◽  
Cell Sheet ◽  
Liver Cells ◽  
Cell Sheets ◽  
Human Liver Cells ◽  
Insulin Producing Cells ◽  
Differentiation Efficiency

Abstract Background: Although pancreatic islet transplantation therapy is ideal for diabetes patients, several hurdles have prevented it from becoming a standard treatment, including donor shortage and low engraftment efficacy. In this study, we prepared insulin-producing cells trans-differentiated from adult human liver cells as a new islet source. Also, cell sheets formation could improve differentiation efficiency and graft survival.Methods: Liver cells were expanded in vitro and trans-differentiated to IPCs using adenovirus vectors carrying human genes for PDX1, NEUROD1 and MAFA. IPCs were seeded on temperature-responsive culture dishes to form cell sheets. Differentiation efficiency were confirmed by ß cell-specific gene expression, insulin production, and immunohistochemistry. IPCs suspension was injected by portal vein (PV), and IPCs sheet was transplanted on the liver surface of the diabetic nude mouse. The therapeutic effect of IPC sheet was evaluated by comparing blood glucose control, weight gain, histological evaluation and hepatotoxicity with IPCs injection group. Also, cell biodistribution was assessed by in vivo/ex vivo fluorescence image tagging.Results: Insulin gene expression and protein production were significantly increased on IPC sheets compared with those in IPCs cultured on conventional culture dishes. Transplanted IPC sheets displayed significantly higher engraftment efficiency and fewer transplanted cells in other organs than injected IPCs, and also lower liver toxicity, improved blood glucose levels, and weight gain. One and two weeks following IPC sheet transplantation, immunohistochemical analyses of liver tissue revealed positive staining for PDX1 and insulin.Conclusions: In conclusion, cell sheet formation enhanced the differentiation function and maturation of IPCs in vitro. Additionally, parameters for clinical application such as distribution, therapeutic efficacy, and toxicity were favorable. The cell sheet technique may be used with IPCs derived from various cell sources in clinical applications.

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