Silk sericin microcapsules with hydroxyapatite shells: protection and modification of organic microcapsules by biomimetic mineralization

2016 ◽  
Vol 4 (2) ◽  
pp. 340-347 ◽  
Author(s):  
Wenhua Li ◽  
Yurong Cai ◽  
Qiwei Zhong ◽  
Ying Yang ◽  
Subhas C. Kundu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Silk Protein ◽  
Phosphate Solution ◽  
Biomimetic Mineralization ◽  
Inorganic Hybrid ◽  
Silk Sericin

Silk protein sericin based organic–inorganic hybrid microcapsules are fabricated by incubating sericin microcapsules with a supersaturated calcium phosphate solution containing citric acid.

scholarly journals Heterogeneous nucleation of hydroxyapatite on protein: structural effect of silk sericin

2005 ◽  
Vol 2 (4) ◽  
pp. 373-378 ◽  
Author(s):  
Akari Takeuchi ◽  
Chikara Ohtsuki ◽  
Toshiki Miyazaki ◽  
Masanobu Kamitakahara ◽  
Shin-ichi Ogata ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Heterogeneous Nucleation ◽  
Structural Effect ◽  
Phosphate Solution ◽  
Mineral Formation ◽  
Silk Sericin ◽  
Sheet Structure ◽  
Acidic Proteins ◽  
The Relationship ◽  
Β Sheet

Acidic proteins play an important role during mineral formation in biological systems, but the mechanism of mineral formation is far from understood. In this paper, we report on the relationship between the structure of a protein and hydroxyapatite deposition under biomimetic conditions. Sericin, a type of silk protein, was adopted as a suitable protein for studying structural effect on hydroxyapatite deposition, since it forms a hydroxyapatite layer on its surface in a metastable calcium phosphate solution, and its structure has been reported. Sericin effectively induced hydroxyapatite nucleation when it has high molecular weight and a β sheet structure. This indicates that the specific structure of a protein can effectively induce heterogeneous nucleation of hydroxyapatite in a biomimetic solution, i.e. a metastable calcium phosphate solution. This finding is useful in understanding biomineralization, as well as for the design of organic polymers that can effectively induce hydroxyapatite nucleation.

scholarly journals Development of A Nano-Apatite Based Composite Sealer for Endodontic Root Canal Filling

2021 ◽  
Vol 5 (1) ◽  
pp. 30
Author(s):  
Angelica Bertacci ◽  
Daniele Moro ◽  
Gianfranco Ulian ◽  
Giovanni Valdrè
Keyword(s):  
Citric Acid ◽  
Liquid Phase ◽  
Calcium Phosphate ◽  
Root Canal ◽  
Acid Attack ◽  
Root Canal Filling ◽  
Bioactive Materials ◽  
Dentinal Tubules ◽  
Sealing Capacity ◽  
Endodontic Sealers

Recently, endodontic sealers based on injectable bioactive materials were proposed to improve the filling of anatomical irregularities during root canal obturation. In this context, this preliminary work investigated the possibility of realizing a new calcium phosphate-based composite sealer for root canal filling with an optimized composition on setting kinetics and dentin tubules occlusion. Several calcium phosphate/liquid phase mixtures were initially evaluated for their workability, finding two suitable formulations. Both of them contained 66 wt.% of a nano-apatite-based cement (solid powdered phase). The liquid phase (34 wt.%) comprised 13.6% propanediol and 20.4% PEG 1000 (formulation 1), and formulation 2 comprised 27.2% glycerin and 6.8% PEG 200 (formulation 2). Then, these formulations were tested by means of permeability measurements and observation by scanning electron microscopy of treated model dentin samples. Both formulations succeeded in occluding dentinal tubules: the first one was able to create a full-bodied layer on dentin surface and, moreover, to resist, at least to a large extent, against citric acid attack. The second one showed a lower effectiveness after citric acid exposure. The composite compound that better satisfied the overall required characteristics of use, workability and sealing capacity was formulation 1.

Calcium phosphate-based organic–inorganic hybrid nanocarriers with pH-responsive on/off switch for photodynamic therapy

2016 ◽  
Vol 4 (5) ◽  
pp. 826-838 ◽  
Author(s):  
Takahiro Nomoto ◽  
Shigeto Fukushima ◽  
Michiaki Kumagai ◽  
Kozo Miyazaki ◽  
Aki Inoue ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Calcium Phosphate ◽  
Ph Responsive ◽  
Inorganic Hybrid ◽  
Normal Tissues

Organic–inorganic hybrid nanocarriers permit efficient photodynamic therapy with reduced damage to normal tissues.

scholarly journals Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 992
Author(s):  
Song Chen ◽  
Yuanli He ◽  
Linna Zhong ◽  
Wenjia Xie ◽  
Yiyuan Xue ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Photoelectron Spectroscopy ◽  
Antibacterial Properties ◽  
Biomimetic Mineralization ◽  
Matrix Mineralization ◽  
X Ray ◽  
Porous Ti ◽  
Potential Applications ◽  
Modification Of Titanium

The surface modification of titanium (Ti) can enhance the osseointegration and antibacterial properties of implants. In this study, we modified porous Ti discs with calcium phosphate (CaP) and different concentrations of Lactoferrin (LF) by biomimetic mineralization and examined their antibacterial effects and osteogenic bioactivity. Firstly, scanning electron microscopy (SEM), the fluorescent tracing method, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and the releasing kinetics of LF were utilized to characterize the modified Ti surface. Then, the antibacterial properties against S. sanguis and S. aureus were investigated. Finally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell differentiation, extracellular matrix mineralization, and cytotoxicity. The results showed that the porous Ti discs were successfully modified with CaP and LF, and that the LF-M group (200 μg/mL LF in simulated body fluid) could mildly release LF under control. Further, the LF-M group could effectively inhibit the adhesion and proliferation of S. sanguis and S. aureus and enhance the osteogenic differentiation in vitro with a good biocompatibility. Consequently, LF-M-modified Ti may have potential applications in the field of dental implants to promote osseointegration and prevent the occurrence of peri-implantitis.

Facile Fabrication of Organic–Inorganic Hybrid Beads by Aminated Alginate Enabled Gelation and Biomimetic Mineralization

2012 ◽  
Vol 24 (2) ◽  
pp. 119-134 ◽  
Author(s):  
Jian Li ◽  
Hong Wu ◽  
Yanpeng Liang ◽  
Zhongyi Jiang ◽  
Yanjun Jiang ◽  
...  
Keyword(s):  
Biomimetic Mineralization ◽  
Inorganic Hybrid ◽  
Facile Fabrication

Comparison of potassium citrate, citric acid and placebo on calcium phosphate stones recurrence: Preliminary results

2018 ◽  
Vol 17 (2) ◽  
pp. e439-e440
Author(s):  
S. Doizi ◽  
J. Poindexter ◽  
M. Pearle ◽  
K. Sakhaee ◽  
N. Maalouf
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Potassium Citrate ◽  
Preliminary Results

A comparative study of ultrasonic degumming of silk sericin using citric acid, sodium carbonate and papain

2019 ◽  
Vol 135 (3) ◽  
pp. 195-201 ◽  
Author(s):  
Wuchao Wang ◽  
Yi Pan ◽  
Kang Gong ◽  
Qi Zhou ◽  
Tonghua Zhang ◽  
...  
Keyword(s):  
Citric Acid ◽  
Comparative Study ◽  
Sodium Carbonate ◽  
Silk Sericin
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