High-efficiency immunoassay platforms with controllable surface roughness and oriented antibody immobilization

2015 ◽  
Vol 3 (38) ◽  
pp. 7499-7502 ◽  
Author(s):  
Lingjie Song ◽  
Jie Zhao ◽  
Shifang Luan ◽  
Jiao Ma ◽  
Weihua Ming ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Specific Antibody ◽  
Boronic Acid ◽  
Particle Deposition ◽  
High Efficiency ◽  
Layer By Layer ◽  
Antibody Immobilization ◽  
Site Specific

High-efficiency immunoassay platforms were facilely prepared by combining a layer-by-layer particle deposition with site-specific antibody immobilization through boronic acid moieties.

scholarly journals Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies

Sensors ◽  
2014 ◽  
Vol 14 (2) ◽  
pp. 2239-2258 ◽  
Author(s):  
Maria Soler ◽  
M.-Carmen Estevez ◽  
Mar Alvarez ◽  
Marinus Otte ◽  
Borja Sepulveda ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Direct Detection ◽  
Antibody Immobilization ◽  
Protein Biomarkers ◽  
Site Specific ◽  
Human Fluids

scholarly journals Investigation of Multiparameter Laser Stripping of AlTiN and DLC C Coatings

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 951
Author(s):  
Tomáš Primus ◽  
Josef Hlavinka ◽  
Pavel Zeman ◽  
Jan Brajer ◽  
Martin Šorm ◽  
...  
Keyword(s):  
Surface Roughness ◽  
High Efficiency ◽  
Steel Substrate ◽  
Cutting Tools ◽  
High Hardness ◽  
Working Time ◽  
Nanosecond Laser ◽  
Altin Coating ◽  
Successful Removal ◽  
Frequency Laser

The lifetime and properties of cutting tools and forming moulds can be prolonged and enhanced by the deposition of hard, thin coatings. After a certain period of usage, the coating will deteriorate. Any remaining coating must be removed prior to successful recoating. Laser stripping is a fast and environmentally friendly coating removal method. In this paper, we present laser removal of two types of coatings deposited on a 1.2379 tool steel substrate, namely, an AlTiN coating with high hardness and a DLC C coating with a small coefficient of friction (COF). A powerful nanosecond laser was employed to remove the coating from the substrate with high efficiency, along with suitable residual surface roughness. Measurements were taken of surface roughness, removed depth, and working time on a stripped area of 1 cm2. The samples were evaluated under a microscope, with a 3D profilometer, and by EDS chemical analysis. Successful removal of the coating was confirmed by optical analysis, but detailed chemical characterisation showed that about 30% of the coating element may remain on the surface. Moreover, a working time of less than 7.5 s per cm2 was obtained in this study. In addition, it was shown that the application of a second low energy, high frequency laser beam pass leads to remelting of the peaks of the material and reduced surface roughness.

scholarly journals Experimental Investigation and Comparison of the Thermal Performance of Additively and Conventionally Manufactured Heat Exchangers

Metals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 574
Author(s):  
Ana Vafadar ◽  
Ferdinando Guzzomi ◽  
Kevin Hayward
Keyword(s):  
Surface Roughness ◽  
Thermal Performance ◽  
Heat Exchangers ◽  
High Efficiency ◽  
Cooling System ◽  
Fluid Dynamic ◽  
Dynamic Performance ◽  
Experimental Studies ◽  
Manufacturing Method ◽  
Industrial Sectors

Air heat exchangers (HXs) are applicable in many industrial sectors because they offer a simple, reliable, and cost-effective cooling system. Additive manufacturing (AM) systems have significant potential in the construction of high-efficiency, lightweight HXs; however, HXs still mainly rely on conventional manufacturing (CM) systems such as milling, and brazing. This is due to the fact that little is known regarding the effects of AM on the performance of AM fabricated HXs. In this research, three air HXs comprising of a single fin fabricated from stainless steel 316 L using AM and CM methods—i.e., the HXs were fabricated by both direct metal printing and milling. To evaluate the fabricated HXs, microstructure images of the HXs were investigated, and the surface roughness of the samples was measured. Furthermore, an experimental test rig was designed and manufactured to conduct the experimental studies, and the thermal performance was investigated using four characteristics: heat transfer coefficient, Nusselt number, thermal fluid dynamic performance, and friction factor. The results showed that the manufacturing method has a considerable effect on the HX thermal performance. Furthermore, the surface roughness and distribution, and quantity of internal voids, which might be created during and after the printing process, affect the performance of HXs.

scholarly journals Modeling the Layer-by-Layer Growth of HKUST-1 Metal-Organic Framework Thin Films

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1631
Author(s):  
Qiang Zhang ◽  
Yohanes Pramudya ◽  
Wolfgang Wenzel ◽  
Christof Wöll
Keyword(s):  
Thin Films ◽  
Surface Roughness ◽  
Deposition Rate ◽  
Layer Growth ◽  
Coarse Grained ◽  
Structural Defects ◽  
Effect Of Temperature ◽  
Layer By Layer ◽  
Reactant Concentration ◽  
Metal Organic

Metal organic frameworks have emerged as an important new class of materials with many applications, such as sensing, gas separation, drug delivery. In many cases, their performance is limited by structural defects, including vacancies and domain boundaries. In the case of MOF thin films, surface roughness can also have a pronounced influence on MOF-based device properties. Presently, there is little systematic knowledge about optimal growth conditions with regard to optimal morphologies for specific applications. In this work, we simulate the layer-by-layer (LbL) growth of the HKUST-1 MOF as a function of temperature and reactant concentration using a coarse-grained model that permits detailed insights into the growth mechanism. This model helps to understand the morphological features of HKUST-1 grown under different conditions and can be used to predict and optimize the temperature for the purpose of controlling the crystal quality and yield. It was found that reactant concentration affects the mass deposition rate, while its effect on the crystallinity of the generated HKUST-1 film is less pronounced. In addition, the effect of temperature on the surface roughness of the film can be divided into three regimes. Temperatures in the range from 10 to 129 °C allow better control of surface roughness and film thickness, while film growth in the range of 129 to 182 °C is characterized by a lower mass deposition rate per cycle and rougher surfaces. Finally, for T larger than 182 °C, the film grows slower, but in a smooth fashion. Furthermore, the potential effect of temperature on the crystallinity of LbL-grown HKUST-1 was quantified. To obtain high crystallinity, the operating temperature should preferably not exceed 57 °C, with an optimum around 28 °C, which agrees with experimental observations.

scholarly journals Effect of Optical and Morphological Control of Single-Structured LEC Device

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 843
Author(s):  
Woo Jin Jeong ◽  
Jong Ik Lee ◽  
Hee Jung Kwak ◽  
Jae Min Jeon ◽  
Dong Yeol Shin ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Surface Roughness ◽  
Surface Properties ◽  
High Efficiency ◽  
High Surface ◽  
Reduction Rate ◽  
Operating Characteristics ◽  
Emission Layer ◽  
Light Emitting ◽  
Stable Operating

We investigated the performance of single-structured light-emitting electrochemical cell (LEC) devices with Ru(bpy)3(PF6)2 polymer composite as an emission layer by controlling thickness and heat treatment. When the thickness was smaller than 120–150 nm, the device performance decreased because of the low optical properties and non-dense surface properties. On the other hand, when the thickness was over than 150 nm, the device had too high surface roughness, resulting in high-efficiency roll-off and poor device stability. With 150 nm thickness, the absorbance increased, and the surface roughness was low and dense, resulting in increased device characteristics and better stability. The heat treatment effect further improved the surface properties, thus improving the device characteristics. In particular, the external quantum efficiency (EQE) reduction rate was shallow at 100 °C, which indicates that the LEC device has stable operating characteristics. The LEC device exhibited a maximum luminance of 3532 cd/m2 and an EQE of 1.14% under 150 nm thickness and 100 °C heat treatment.

A benchtop capillary flow layer-by-layer (CF-LbL) platform for rapid assembly and screening of biodegradable nanolayered films

Lab on a Chip ◽  
2016 ◽  
Vol 16 (23) ◽  
pp. 4601-4611 ◽  
Author(s):  
Ziye Dong ◽  
Ling Tang ◽  
Caroline C. Ahrens ◽  
Zhenya Ding ◽  
Vi Cao ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Film Thickness ◽  
Capillary Flow ◽  
Layer By Layer

We report a benchtop platform to systematically study film thickness, surface roughness, biocompatibility and degradation of a series of biodegradable nanolayered films.

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