The influence of polymer architecture on in vitro pDNA transfection

2015 ◽  
Vol 3 (38) ◽  
pp. 7477-7493 ◽  
Author(s):  
Alexandra C. Rinkenauer ◽  
Stephanie Schubert ◽  
Anja Traeger ◽  
Ulrich S. Schubert
Keyword(s):  
Block Copolymers ◽  
Gene Delivery ◽  
Polymer Architecture ◽  
Good Biocompatibility ◽  
Self Assembled

In the field of polymer-based gene delivery, the tuning potential of polymers by using different architectures like graft- and star-shaped polymers as well as self-assembled block copolymers is immense. In the last years numerous new polymer designs showed enhanced transfections properties in combination with a good biocompatibility.

Synthesis and Characterization of Polyethylenimine-Graft-Poly(L-Lactide-Co-Glycolide) Block Copolymers for Gene Delivery

2007 ◽  
Vol 342-343 ◽  
pp. 521-524
Author(s):  
Oju Jeon ◽  
Su Jin Song ◽  
Min Hyung Lee ◽  
Sang Woo Seo ◽  
Cha Yong Choi ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gel Permeation Chromatography ◽  
Copolymer Composition ◽  
High Gene ◽  
Good Biocompatibility ◽  
Delivery Vehicles

Polyethylenimine-graft-poly(L-lactide-co-glycolide) (PEI-g-PLGA) block copolymers were prepared by a ring-opening polymerization of L-lactide and glycolide using PEI as a macroinitiator and stannous octoate as a catalyst in dimethylformamide at 100 °C. The molecular structure of the block copolymers was evaluated with 1H-NMR, and the molecular weight of the block copolymers was determined with gel permeation chromatography. The thermal properties were investigated using differential scanning calorimetery and thermogravimetric analysis. The zetapotential of the pDNA/copolymer complexes was evaluated with dynamic laser light scattering. Cytotoxicity and gene transfection efficiency of PEI-g-PLGA were tested in vitro using human embryonic kidney 293 cell culture. The pDNA/copolymer complexes (N/P = 10) showed a lower zeta-potential than pDNA/PEI25kDa complex, suggesting the lower toxicity of the pDNA/copolymer complexes. The copolymer composition was found to significantly affect the gene transfection efficiency of the pDNA/copolymer complexes. The copolymers with lower contents of PLGA showed higher gene transfection efficiency. These results indicate that these block copolymers are promising candidates for gene delivery vehicles, featuring good biocompatibility, potential biodegradability, and relatively high gene transfection efficiency.

Poly(imidazole/DMAEA)phosphazene/DNA self-assembled nanoparticles for gene delivery: Synthesis and in vitro transfection

2008 ◽  
Vol 127 (3) ◽  
pp. 273-279 ◽  
Author(s):  
Yongxin Yang ◽  
Zhenghong Xu ◽  
Jingui Jiang ◽  
Yu Gao ◽  
Wangwen Gu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Self Assembled ◽  
In Vitro Transfection

scholarly journals Tuning the hydrophobic cores of self-immolative polyglyoxylate assemblies

2018 ◽  
Vol 9 (19) ◽  
pp. 2601-2610 ◽  
Author(s):  
Bo Fan ◽  
Rebecca E. Yardley ◽  
John F. Trant ◽  
Aneta Borecki ◽  
Elizabeth R. Gillies
Keyword(s):  
Block Copolymers ◽  
Drug Carriers ◽  
Amphiphilic Block Copolymers ◽  
Release Rates ◽  
Self Assembled ◽  
Hydrophobic Cores

Amphiphilic block copolymers containing different self-immolative polyglyoxylates were synthesized and self-assembled to provide drug carriers with variable celecoxib loading capacities and release rates, as well as different in vitro toxicities.

Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro

2004 ◽  
Vol 97 (3) ◽  
pp. 551-566 ◽  
Author(s):  
B TWAITES ◽  
C DELASHERASALARCON ◽  
D CUNLIFFE ◽  
M LAVIGNE ◽  
S PENNADAM ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ph Responsive ◽  
Polymer Architecture ◽  
Responsive Polymers ◽  
Gene Delivery Vectors ◽  
Ph Responsive Polymers

Injectable self-assembled block copolymers for sustained gene and drug co-delivery: An in vitro study

2012 ◽  
Vol 427 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Bingqi Zhang ◽  
Feng Jia ◽  
Michael Q. Fleming ◽  
Surya K. Mallapragada
Keyword(s):  
Block Copolymers ◽  
In Vitro Study ◽  
Vitro Study ◽  
Self Assembled

Adenoviral gene delivery of interleukin-10 inhibits kupffer cells avtivation in vitro

2007 ◽  
Vol 45 (01) ◽  
Author(s):  
P Walbrun ◽  
S Netter ◽  
R Wiest ◽  
E Gäbele ◽  
J Schölmerich ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Kupffer Cells ◽  
Interleukin 10 ◽  
Adenoviral Gene Delivery

Exosome as a Natural Gene Delivery Vector for Cancer Treatment

2020 ◽  
Vol 20 (11) ◽  
pp. 821-830
Author(s):  
Prasad Pofali ◽  
Adrita Mondal ◽  
Vaishali Londhe
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Cancer Treatment ◽  
Intestinal Barrier ◽  
Gene Carrier ◽  
Gene Delivery Vector ◽  
Delivery Vector

Background: Current gene therapy vectors such as viral, non-viral, and bacterial vectors, which are used for cancer treatment, but there are certain safety concerns and stability issues of these conventional vectors. Exosomes are the vesicles of size 40-100 nm secreted from multivesicular bodies into the extracellular environment by most of the cell types in-vivo and in-vitro. As a natural nanocarrier, exosomes are immunologically inert, biocompatible, and can cross biological barriers like the blood-brain barrier, intestinal barrier, and placental barrier. Objective: This review focusses on the role of exosome as a carrier to efficiently deliver a gene for cancer treatment and diagnosis. The methods for loading of nucleic acids onto the exosomes, advantages of exosomes as a smart intercellular shuttle for gene delivery and therapeutic applications as a gene delivery vector for siRNA, miRNA and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and also the limitations of exosomes as a gene carrier are all reviewed in this article. Methods: Mostly, electroporation and chemical transfection are used to prepare gene loaded exosomes. Results: Exosome-mediated delivery is highly promising and advantageous in comparison to the current delivery methods for systemic gene therapy. Targeted exosomes, loaded with therapeutic nucleic acids, can efficiently promote the reduction of tumor proliferation without any adverse effects. Conclusion: In the near future, exosomes can become an efficient gene carrier for delivery and a biomarker for the diagnosis and treatment of cancer.

scholarly journals Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 781 ◽  
Author(s):  
Paula E. Florian ◽  
Liviu Duta ◽  
Valentina Grumezescu ◽  
Gianina Popescu-Pelin ◽  
Andrei C. Popescu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Doping Agent ◽  
Hydroxyapatite Coatings ◽  
3D Network ◽  
Immunofluorescence Labelling ◽  
Good Biocompatibility ◽  
Adhesion Process

This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.

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