scholarly journals Nanocoating for biomolecule delivery using layer-by-layer self-assembly

2015 ◽  
Vol 3 (45) ◽  
pp. 8757-8770 ◽  
Author(s):  
M. Keeney ◽  
X. Y. Jiang ◽  
M. Yamane ◽  
M. Lee ◽  
S. Goodman ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Layer By Layer ◽  
Biological Interactions

Schematic illustrates the formation of LbL coatings using different methods. (A) Electrostatic interactions; (B) Hydrogen bonding; or (C) Biological interactions.

Hydrogen-Bonding Layer Protecting Polyelectrolyte Capsules and Its Mechanical Property Study with Atomic Force Microscope

2008 ◽  
Vol 8 (6) ◽  
pp. 2996-3002 ◽  
Author(s):  
Liqin Ge ◽  
Xing Wang ◽  
Long Ba ◽  
Zhongze Gu
Keyword(s):  
Hydrogen Bonding ◽  
Atomic Force Microscope ◽  
Elastic Deformation ◽  
Self Assembly ◽  
Laser Scanning ◽  
Confocal Laser ◽  
Bonding Layer ◽  
Layer By Layer ◽  
Assembly Method ◽  
Atomic Force

The hydrogen-bonding multilayered polyelectrolyte capsules with sizes around 6 μm were fabricated by layer-by-layer self-assembly method. The morphology of the obtained capsules was observed with Scanning Electron Microscope (SEM), Confocal Laser Scanning Microscope (CLSM) and Atomic Force Microscope (AFM), respectively. The elastic properties of the capsules were studied with AFM. The capsule was pressed by cantilever with different lengths, a glass bead glued at the end of the cantilever. The force curves were measured on the capsule in air. The Young's modulus of the capsule was obtained (E = 170 MPa for the loading). Results show that this model can predict the elastic deformation of the microcapsule. The accuracy of the elastic deformation of polymer capsule can be ensured using a cantilever of mediate stiffness. Our results show that the existence of the hydrogen-bonding layer makes the multilayered polyelectrolyte harder in comparison with the pure multilayered polyelectrolyte capsules.

Adsoption CD34 Antibody onto the Col/Hep Coating Film on Titanium to Improve Cytocompatibility of Titanium

2008 ◽  
Vol 47-50 ◽  
pp. 1411-1414 ◽  
Author(s):  
Jia Long Chen ◽  
Quan Li Li ◽  
Jun Ying Chen ◽  
Nan Huang
Keyword(s):  
Self Assembly ◽  
Electrostatic Interactions ◽  
Layer By Layer ◽  
Coating Film ◽  
Test Results ◽  
Cardiovascular Devices ◽  
Assembly Technique ◽  
Confluent Cell ◽  
Good Potential ◽  
Positively Charged

This study deals with improving the cytocompatibility of titanium by a coating with heparin(hep), collagen(col) and CD34 antibody. Collagen and heparin molecules were adsorbed onto a titanium surface using a layer-by-layer (LBL) self-assembly technique. The col/hep coating were formed by alternating deposition of negatively charged heparin and positively charged collagen based on electrostatic interactions. Finally the CD34 antibody was absorbed onto the surface of col/hep coating by electrostatic interactions. The chemical composition and surface topography were investigated by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The fluorescence microscopy images proved that the CD34 antibody was existed on the surface of the col/hep coating. The SEM results and the MTT test results showed that, compared to the surface of col/hep coating and the surface of titanium, the CD34 surface had better endothelial progenitor cells (EPCs) attachment and proliferation. The EPCs on the CD34 surface displayed the morphology of flat endothelium, and a confluent cell layer after cultured for 2d. This study suggested that this method maybe have good potential for surface modification of cardiovascular devices.

scholarly journals Hydrogen-Bonding-Driven Self-Assembly of PEGylated Organosilica Nanoparticles with Poly(acrylic acid) in Aqueous Solutions and in Layer-by-Layer Deposition at Solid Surfaces

Langmuir ◽  
2011 ◽  
Vol 28 (1) ◽  
pp. 299-306 ◽  
Author(s):  
Galiya S. Irmukhametova ◽  
Brian J. Fraser ◽  
Joseph L. Keddie ◽  
Grigoriy A. Mun ◽  
Vitaliy V. Khutoryanskiy
Keyword(s):  
Hydrogen Bonding ◽  
Acrylic Acid ◽  
Aqueous Solutions ◽  
Self Assembly ◽  
Solid Surfaces ◽  
Layer By Layer ◽  
Layer Deposition ◽  
Poly Acrylic Acid

Investigation of pH and concentration influence on layer-by-layer self-assembly for nickel(II)phthalocyanine-tetrasulfonic acid tetrasodium salt coatings

2019 ◽  
Vol 23 (01n02) ◽  
pp. 84-90 ◽  
Author(s):  
Yonca Belce ◽  
Fevzi Ç. Cebeci
Keyword(s):  
Thin Film ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Ph Value ◽  
Film Formation ◽  
Layer By Layer ◽  
Lbl Assembly ◽  
Layer Deposition ◽  
Vis Spectroscopy ◽  
Ionic Density

Porphyrins and phthalocyanines are widely studied molecules for various functional applications. Researchers have investigated these photoactive compounds for electrochemical, sensor, semiconductor and photodynamic therapy purposes. Layer-by-layer (LbL) self-assembly is preferred for its simple, environmentally-friendly and water-based features compared to other coating techniques in the literature. Coating thickness can be controlled on the order of nanometers by LbL mechanism. Multilayer thin film formation of diverse phthalocyanine-based molecules is examined in terms of molecular orientation and temperature dependency by the LbL method. However, as well as concentration and temperature, the pH of the coating medium is another challenging parameter in the LbL approach. Film thickness and layer distribution are influenced by pH value, changing ionic density and hence the strength of electrostatic interactions during LbL assembly. In this study, layer-by-layer deposition of branched poly(ethyleneimine)/nickel(II)phthalocyanine-tetrasulfonic acid tetrasodium salt (NiPcTS) coating pair is studied. Impact of pH and concentration of NiPcTS on thin film properties are tested for four different pH conditions. Corresponding analysis is made by UV-vis spectroscopy, surface profiler and quartz-crystal microbalance. LbL deposition of NiPcTS is homogeneously controlled and 98 nm thick films are obtained in the presence of acidic media.

Molecular Layer-by-Layer Self-Assembly of Water-Soluble Perylene Diimides through π−π and Electrostatic Interactions

Langmuir ◽  
2006 ◽  
Vol 22 (1) ◽  
pp. 26-28 ◽  
Author(s):  
Tingji Tang ◽  
Jiangqiang Qu ◽  
Klaus Müllen ◽  
Stephen E. Webber
Keyword(s):  
Self Assembly ◽  
Electrostatic Interactions ◽  
Molecular Layer ◽  
Water Soluble ◽  
Layer By Layer ◽  
Perylene Diimides

Layer-by-Layer Self-Assembly of Modified Hyaluronic Acid/Chitosan Based on Hydrogen Bonding

2009 ◽  
Vol 10 (9) ◽  
pp. 2632-2639 ◽  
Author(s):  
Uttam Manna ◽  
Sri Bharani ◽  
Satish Patil
Keyword(s):  
Hydrogen Bonding ◽  
Hyaluronic Acid ◽  
Self Assembly ◽  
Layer By Layer

In situ Time-Resolved Small-Angle X-ray Scattering Reveals the Formation Kinetics of Polyelectrolyte Complex Micelles

2019 ◽  
Author(s):  
Hao Wu ◽  
Jeffrey Ting ◽  
Siqi Meng ◽  
Matthew Tirrell
Keyword(s):  
Small Angle ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Polyelectrolyte Complex ◽  
Time Resolved ◽  
X Ray ◽  
X Ray Scattering ◽  
Kinetics Of ◽  
Ray Scattering

We have directly observed the <i>in situ</i> self-assembly kinetics of polyelectrolyte complex (PEC) micelles by synchrotron time-resolved small-angle X-ray scattering, equipped with a stopped-flow device that provides millisecond temporal resolution. This work has elucidated one general kinetic pathway for the process of PEC micelle formation, which provides useful physical insights for increasing our fundamental understanding of complexation and self-assembly dynamics driven by electrostatic interactions that occur on ultrafast timescales.

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