Synthesis and characterization of a novel injectable alginate–collagen–hydroxyapatite hydrogel for bone tissue regeneration

2015 ◽  
Vol 3 (15) ◽  
pp. 3081-3090 ◽  
Author(s):  
Stephanie T. Bendtsen ◽  
Mei Wei
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Gelation Time ◽  
Bone Tissue Regeneration ◽  
Synthesis And Characterization ◽  
Injectable Hydrogel ◽  
Surgical Setting ◽  
Hydrogel System

This novel fabrication process allowed for the development of an injectable hydrogel system with a gelation time suitable for a surgical setting and components necessary for promoting enhanced bone regeneration.

Synthesis and characterization of silibinin/phenanthroline/neocuproine copper(II) complexes for augmenting bone tissue regeneration: an in vitro analysis

2018 ◽  
Vol 23 (5) ◽  
pp. 753-762 ◽  
Author(s):  
Subramaniyam Rajalakshmi ◽  
Selvaraj Vimalraj ◽  
Sekaran Saravanan ◽  
Desingh Raj Preeth ◽  
Manickaraj Shairam ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
Synthesis And Characterization ◽  
Vitro Analysis ◽  
In Vitro Analysis

Physicochemical characterization of segmented polyurethanes prepared with glutamine or ascorbic acid as chain extenders and their hydroxyapatite composites

2014 ◽  
Vol 2 (14) ◽  
pp. 1966-1976 ◽  
Author(s):  
S. M. Cetina-Diaz ◽  
L. H. Chan-Chan ◽  
R. F. Vargas-Coronado ◽  
J. M. Cervantes-Uc ◽  
P. Quintana-Owen ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Physicochemical Characterization ◽  
Bone Tissue Regeneration ◽  
Chain Extenders ◽  
Segmented Polyurethanes

Segmented polyurethanes with glutamine or ascorbic acid as chain extenders and their hydroxyapatite composites for bone tissue regeneration.

scholarly journals Bone Regeneration in Critical-Sized Bone Defects Treated with Additively Manufactured Porous Metallic Biomaterials: The Effects of Inelastic Mechanical Properties

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1992
Author(s):  
Marianne Koolen ◽  
Saber Amin Yavari ◽  
Karel Lietaert ◽  
Ruben Wauthle ◽  
Amir A. Zadpoor ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bulk Material ◽  
Bone Tissue Regeneration ◽  
Bony Tissue ◽  
Metallic Biomaterials ◽  
Cp Ti ◽  
Porous Biomaterials

Additively manufactured (AM) porous metallic biomaterials, in general, and AM porous titanium, in particular, have recently emerged as promising candidates for bone substitution. The porous design of such materials allows for mimicking the elastic mechanical properties of native bone tissue and showed to be effective in improving bone regeneration. It is, however, not clear what role the other mechanical properties of the bulk material such as ductility play in the performance of such biomaterials. In this study, we compared the bone tissue regeneration performance of AM porous biomaterials made from the commonly used titanium alloy Ti6Al4V-ELI with that of commercially pure titanium (CP-Ti). CP-Ti was selected because of its high ductility as compared to Ti6Al4V-ELI. Critical-sized (6 mm diameter) femoral defects in rats were treated with implants made from both Ti6Al4V-ELI and CP-Ti. Bone regeneration was assessed up to 11 weeks using micro-CT scanning. The regenerated bone volume was assessed ex vivo followed by histology and biomechanical testing to assess osseointegration of the implants. The bony defects treated with AM CP-Ti implants generally showed higher volumes of regenerated bone as compared to those treated with AM Ti6Al4V-ELI. The torsional strength of the two titanium groups were similar however, and both considerably lower than those measured for intact bony tissue. These findings show the importance of material type and ductility of the bulk material in the ability for bone tissue regeneration of AM porous biomaterials.

scholarly journals Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

2020 ◽  
Vol 21 (6) ◽  
pp. 2175 ◽  
Author(s):  
Alina Lauer ◽  
Philipp Wolf ◽  
Dorothea Mehler ◽  
Hermann Götz ◽  
Mehmet Rüzgar ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Growth ◽  
Collagen Type ◽  
Biomechanical Testing ◽  
Collagen Type I ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Type I

Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.

scholarly journals Marine-Inspired Enzymatic Mineralization of Dairy-Derived Whey Protein Isolate (WPI) Hydrogels for Bone Tissue Regeneration

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 294
Author(s):  
Karl Norris ◽  
Magdalena Kocot ◽  
Anna M. Tryba ◽  
Feng Chai ◽  
Abdullah Talari ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Whey Protein ◽  
Tissue Regeneration ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Biologically Active ◽  
Bone Tissue Regeneration ◽  
Biological Studies

Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and ability to enhance proliferation and osteogenic differentiation of bone-forming cells. Hence, they have promise in the area of bone tissue regeneration. In contrast to commonly used ceramic minerals for bone regeneration, a major advantage of hydrogels is the ease of their modification by incorporating biologically active substances such as enzymes. Calcium carbonate (CaCO3) is the main inorganic component of the exoskeletons of marine invertebrates. Two polymorphs of CaCO3, calcite and aragonite, have shown the ability to promote bone regeneration. Other authors have reported that the addition of magnesium to inorganic phases has a beneficial effect on bone-forming cell growth. In this study, we employed a biomimetic, marine-inspired approach to mineralize WPI hydrogels with an inorganic phase consisting of CaCO3 (mainly calcite) and CaCO3 enriched with magnesium using the calcifying enzyme urease. The novelty of this study lies in both the enzymatic mineralization of WPI hydrogels and enrichment of the mineral with magnesium. Calcium was incorporated into the mineral formed to a greater extent than magnesium. Increasing the concentration of magnesium in the mineralization medium led to a reduction in the amount and crystallinity of the mineral formed. Biological studies revealed that mineralized and unmineralized hydrogels were not cytotoxic and promoted cell viability to comparable extents (approximately 74% of standard tissue culture polystyrene). The presence of magnesium in the mineral formed had no adverse effect on cell viability. In short, WPI hydrogels, both unmineralized and mineralized with CaCO3 and magnesium-enriched CaCO3, show potential as biomaterials for bone regeneration.

Injectable Organic-Inorganic Biocomposites for Bone Tissue Regeneration - A Mini Review

2021 ◽  
Vol 903 ◽  
pp. 52-59
Author(s):  
Inta Kreicberga ◽  
Kristine Salma-Ancane
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Diseases ◽  
Biologically Active ◽  
Bone Tissue Regeneration ◽  
Osteoporotic Bone ◽  
Synthesis Methods ◽  
Active Components ◽  
Large Bone

Bone regeneration is complex physiological process, which include the most common form of regeneration - bone fracture healing and new bone formation. Moreover, large bone defects, infections and bone diseases such as osteoporosis and arthritis can impair bone regeneration. Despite intensive research and development of biomaterials for bone tissue engineering, especially for osteoporotic bone healing, the properties of the fabricated biomaterials are still far from those of unique composite structure of natural bone and desired therapeutic effect not achieved. This mini-review will highlight the various cutting-edge injectable inorganic-organic biocomposites as minimally invasive and regenerative therapeutics for bone tissue regeneration. The review will summarize the main strategic tools for the development of injectable biocomposites: natural or synthetic biopolymer-based hydrogels, bioactive inorganic fillers and biologically active components, as well as the fabrication techniques and synthesis methods.

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