scholarly journals Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

2016 ◽  
Vol 7 (4) ◽  
pp. 2827-2831 ◽  
Author(s):  
Abhishek Santra ◽  
Hai Yu ◽  
Nova Tasnima ◽  
Musleh M. Muthana ◽  
Yanhong Li ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Chemoenzymatic Synthesis ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

O-Sulfated sialyl Lewisxantigens containing different sialic acid forms were chemoenzymatically synthesized using a bacterial sialyltransferase mutant.

scholarly journals Chemoenzymatic synthesis of sialyl Lewis x containing different sialic acid forms

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Ronald Wei Huang ◽  
Kam Lau ◽  
Go Sugiarto ◽  
Vireak Thon ◽  
Yanhong Li ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Chemoenzymatic Synthesis ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

Synthetic Studies on Sialoglycoconjugates 85: Synthesis of Sialyl Lewis X Ganglioside Analogs Containing a Variety of Anionic Substituents in Place of Sialic Acid

1996 ◽  
Vol 15 (4) ◽  
pp. 399-418 ◽  
Author(s):  
Masahiro Yoshida ◽  
Yukiko Kawakami ◽  
Hideharu Ishida ◽  
Makoto Kiso ◽  
Akira Hasegawa
Keyword(s):  
Sialic Acid ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Synthetic Studies

scholarly journals Sialyl-Lewis x-Independent Infection of Human Myeloid Cells by Anaplasma phagocytophilum Strains HZ and HGE1

2007 ◽  
Vol 75 (12) ◽  
pp. 5720-5725 ◽  
Author(s):  
Madhubanti Sarkar ◽  
Dexter V. Reneer ◽  
Jason A. Carlyon
Keyword(s):  
Sialic Acid ◽  
Anaplasma Phagocytophilum ◽  
Myeloid Cells ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Bacterial Populations ◽  
Obligate Intracellular ◽  
Granulocytic Anaplasmosis ◽  
Sialyl Lewis ◽  
N Terminus

ABSTRACT Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, is an obligate intracellular bacterium that infects neutrophils and neutrophil precursors. Bacterial recognition of P-selectin glycoprotein ligand-1 (PSGL-1) and the α2,3-sialylated- and α1,3-fucosylated-moiety sialyl-Lewis x (sLex), which modifies the PSGL-1 N terminus, is important for adhesion to and invasion of myeloid cells. We have previously demonstrated that A. phagocytophilum organisms of the NCH-1 strain that utilize an sLex-modified PSGL-1-independent means of entry can be enriched for by cultivation in undersialylated HL-60 cells that are unable to construct sLex. Because it was unknown whether other A. phagocytophilum isolates share this ability, we extended our studies to the geographically diverse strains HZ and HGE1. HL-60 A2 is a clonal cell line that is defective for sialylation and α1,3-fucosyltransferase. HL-60 A2 cell surfaces, therefore, not only lack sLex but also are virtually devoid of any other sialic acid- and/or α1,3-fucose-modified glycan. By cultivating HZ and HGE1 in HL-60 A2 cells, we enriched for bacterial subpopulations (termed HZA2 and HGE1A2) that bind and/or infect myeloid cells in the absence of sialic acid and α1,3-fucose and in the presence of antibody that blocks the N terminus of PSGL-1. Thus, multiple A. phagocytophilum isolates share the ability to use sLex-modified PSGL-1-dependent and -independent routes of entry into myeloid cells. HZA2 and HGE1A2 represent enriched bacterial populations that will aid dissection of the complexities of the interactions between A. phagocytophilum and host myeloid cells.

Synthesis of a Sialic Acid α(2−3) Galactose Building Block and Its Use in a Linear Synthesis of Sialyl Lewis X

2007 ◽  
Vol 9 (9) ◽  
pp. 1777-1779 ◽  
Author(s):  
Shinya Hanashima ◽  
Bastien Castagner ◽  
Davide Esposito ◽  
Toshiki Nokami ◽  
Peter H. Seeberger
Keyword(s):  
Sialic Acid ◽  
Building Block ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

Chemoenzymatic Synthesis of a PSGL-1 N-Terminal Glycopeptide Containing Tyrosine Sulfate and α-O-Linked Sialyl Lewis X

2000 ◽  
Vol 122 (17) ◽  
pp. 4241-4242 ◽  
Author(s):  
Kathryn M. Koeller ◽  
Mark E. B. Smith ◽  
Rong-Fong Huang ◽  
Chi-Huey Wong
Keyword(s):  
Chemoenzymatic Synthesis ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

Chemoenzymatic synthesis of GM3, Lewis x and sialyl Lewis x oligosaccharides in 13C-enriched form

1997 ◽  
Vol 38 (33) ◽  
pp. 5861-5864 ◽  
Author(s):  
Mark A. Probert ◽  
Mark.J. Milton ◽  
Richard Harris ◽  
Sergio Schenkman ◽  
Jonathan M. Brown ◽  
...  
Keyword(s):  
Chemoenzymatic Synthesis ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis

Chemoenzymatic synthesis of l-galactosylated dimeric Sialyl Lewis X structures employing α-1,3-fucosyltransferase V

2000 ◽  
Vol 8 (10) ◽  
pp. 2519-2525 ◽  
Author(s):  
Arno Düffels ◽  
Luke G. Green ◽  
Roman Lenz ◽  
Steven V. Ley ◽  
Stephane P. Vincent ◽  
...  
Keyword(s):  
Chemoenzymatic Synthesis ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis
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