scholarly journals Two-color imaging of microRNA with enzyme-free signal amplification via hybridization chain reactions in living cells

2016 ◽  
Vol 7 (3) ◽  
pp. 1940-1945 ◽  
Author(s):  
Lu Li ◽  
Jie Feng ◽  
Haiyun Liu ◽  
Qingling Li ◽  
Lili Tong ◽  
...  
Keyword(s):  
Disease Diagnosis ◽  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Early Disease ◽  
Chain Reaction ◽  
Chain Reactions ◽  
Simultaneous Imaging ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Non Destructive

Here, a non-destructive amplification strategy is developed to image miRNAs in living cells, utilizing the enzyme-free hybridization chain reaction (HCR) with graphene oxide (GO) as a carrier. This provides a new tool for highly sensitive and simultaneous imaging of multiple low-level biomarkers, improving the accuracy of early disease diagnosis.

A triple-amplification strategy based on the formation of peroxidase-like two-dimensional DNA/Fe3O4 networks initiated by the hybridization chain reaction for highly sensitive detection of microRNA

2019 ◽  
Vol 55 (58) ◽  
pp. 8386-8389 ◽  
Author(s):  
Sheng Tang ◽  
Yana Li ◽  
Anni Zhu ◽  
Yao Yao ◽  
Jun Sun ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Hybridization Chain Reaction ◽  
Two Dimensional ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Peroxidase-like two-dimensional DNA/Fe3O4 networks were constructed by the hybridization chain reaction and applied to detect microRNA down to the aM level.

Biodegradable MnO2 nanosheet mediated hybridization chain reaction for imaging of human apurinic/apyrimidinic endonuclease 1 activity in living cells

Nanoscale ◽  
2019 ◽  
Vol 11 (43) ◽  
pp. 20456-20460 ◽  
Author(s):  
Guangli Li ◽  
Junjie Li ◽  
Qing Li
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Amplification Assay ◽  
Mno2 Nanosheet

A highly sensitive enzyme-free amplification assay for the detection of apurinic/apyrimidinic endonuclease 1 activity was developed.

Lab in a tube: a fast-assembled colorimetric sensor for highly sensitive detection of oligonucleotides based on a hybridization chain reaction

RSC Advances ◽  
2015 ◽  
Vol 5 (55) ◽  
pp. 44714-44721 ◽  
Author(s):  
Siqi Zhang ◽  
Kun Wang ◽  
Zhenyu Li ◽  
Zhongmin Feng ◽  
Ting Sun
Keyword(s):  
Complex Formation ◽  
Dna Sequence ◽  
Self Assembly ◽  
The Self ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Colored Product ◽  
Highly Sensitive Detection

Upon adding THBV, the self-assembly of THBV with H1 allows the rest of the DNA sequence of H1 to accelerate H1–H2 complex formation. The G-quadruplex at the end of the H1–H2 complex could catalyze TMB into a colored product.

Imaging of Receptor Dimers in Zebrafish and Living Cells via Aptamer Recognition and Proximity-Induced Hybridization Chain Reaction

2018 ◽  
Vol 90 (24) ◽  
pp. 14433-14438 ◽  
Author(s):  
Liping Wang ◽  
Wei Li ◽  
Jin Sun ◽  
Su-Yun Zhang ◽  
Sheng Yang ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Receptor Dimers

A novel FRET-based dendritic hybridization chain reaction for tumour-related mRNA imaging

2022 ◽  
Author(s):  
Shiyuan Liu ◽  
Zhenxiang Liu ◽  
Huimin Wang ◽  
Jiaoli Wang ◽  
Ruiting Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A novel FRET-based dendritic hybridization chain reaction (D-HCR) for TK1 mRNA imaging was developed in living cells. Compared to the traditional complex D-HCR methods, it has the following advantages, including...

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