Hydroxyapatite-containing silk fibroin nanofibrous scaffolds for tissue-engineered periosteum

RSC Advances ◽  
2016 ◽  
Vol 6 (23) ◽  
pp. 19463-19474 ◽  
Author(s):  
Xili Ding ◽  
Chengqi Wu ◽  
Tong Ha ◽  
Lizhen Wang ◽  
Yan Huang ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Nanofibrous Scaffold ◽  
Nanofibrous Scaffolds ◽  
One Step

A simple, one-step technology is developed to generate a hydroxyapatite (HA)-containing silk fibroin nanofibrous scaffold which has great potential as osteogenesis promoting scaffolds for constructing tissue-engineered periosteum.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

Fabrication and Characterization of Vitamin E TPGS Loaded Silk Fibroin/Hyaluronic Acid Nanofibrous Scaffolds

2013 ◽  
Vol 721 ◽  
pp. 274-277
Author(s):  
Li Li Ji ◽  
Qiao Ling Li ◽  
Zeng Hu Yang ◽  
Wei Jing Hu ◽  
Kui Hua Zhang
Keyword(s):  
Hyaluronic Acid ◽  
Vitamin E ◽  
Silk Fibroin ◽  
Ethanol Vapor ◽  
Random Coil ◽  
Burst Release ◽  
Nanofibrous Scaffolds ◽  
Vitamin E Tpgs ◽  
Polyethylene Glycol 1000 ◽  
Β Sheet

Vitamin E d-alpha-tocopheryl polyethylene glycol 1000 succinate (VE TPGS) loaded silk fibroin (SF)/ hyaluronic acid (HA) nanofibrous scaffolds were fabricated by means of electrospinning to biomimic the natural extracellular matrix. Scanning electronic microscopy (SEM) results indicated that electrospun VE TPGS loaded SF/HA nanofibers were ribbon-shaped, the width of nanofibers decreased slightly with the addition of VE TPGS to SF/HA blended solutions. Fourier transform infrared (FTIR) spectroscopy and Wide-angle X-ray diffraction (WAXD) curves revealed that VE TPGS did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. In vitro release studies confirmed VE TPGS had no obvious burst release and present good release behavior.

Genipin-crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering application

2010 ◽  
Vol 95A (3) ◽  
pp. 870-881 ◽  
Author(s):  
Kuihua Zhang ◽  
Yongfang Qian ◽  
Hongsheng Wang ◽  
Linpeng Fan ◽  
Chen Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Nanofibrous Scaffolds

scholarly journals Immobilization of silk fibroin on the surface of PCL nanofibrous scaffolds for tissue engineering applications

2018 ◽  
Vol 135 (37) ◽  
pp. 46684 ◽  
Author(s):  
Alireza Khosravi ◽  
Laleh Ghasemi-Mobarakeh ◽  
Hossein Mollahosseini ◽  
Fatemeh Ajalloueian ◽  
Maryam Masoudi Rad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Engineering Applications ◽  
Nanofibrous Scaffolds

Green electrospun nanocuprous oxide–poly(ethylene oxide)–silk fibroin composite nanofibrous scaffolds for antibacterial dressings

2019 ◽  
Vol 136 (28) ◽  
pp. 47730 ◽  
Author(s):  
Zexin Zheng ◽  
Kuihua Zhang ◽  
Bo Wu ◽  
Haoyi Yang ◽  
Mengqi Wang ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Silk Fibroin ◽  
Nanofibrous Scaffolds ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

Preparation and characterization of polyhydroxybutyrate-co -hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering

2014 ◽  
Vol 55 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Caihong Lei ◽  
Hailin Zhu ◽  
Jingjing Li ◽  
Jiuming Li ◽  
Xinxing Feng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds
Sign in / Sign up

Export Citation Format

Share Document