Colorimetric detection of Maize chlorotic mottle virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynapthol blue dye

RSC Advances ◽  
2016 ◽  
Vol 6 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Zhanmin Liu ◽  
Xueying Xia ◽  
Cuiyun Yang ◽  
Junyi Huang
Keyword(s):  
Reverse Transcription ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Mottle Virus ◽  
Blue Dye ◽  
Maize Seeds ◽  
Chlorotic Mottle Virus ◽  
Maize Chlorotic Mottle Virus ◽  
Chlorotic Mottle

Maize chlorotic mottle virus causes corn lethal necrosis disease, and can be transmitted via infected maize seeds. A colorimetric assay for the detection of Maize chlorotic mottle virus was developed which utilises RT-LAMP and hydroxynapthol blue dye (HNB).

Visual detection of Maize chlorotic mottle virus by asymmetric polymerase chain reaction with unmodified gold nanoparticles as the colorimetric probe

2016 ◽  
Vol 8 (38) ◽  
pp. 6959-6964 ◽  
Author(s):  
Lin Wang ◽  
Zhanmin Liu ◽  
Xueying Xia ◽  
Junyi Huang
Keyword(s):  
Polymerase Chain Reaction ◽  
Visual Detection ◽  
Mottle Virus ◽  
Chain Reaction ◽  
Maize Seeds ◽  
Chlorotic Mottle Virus ◽  
Maize Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Asymmetric Polymerase Chain Reaction ◽  
Polymerase Chain

Maize chlorotic mottle virus causes corn lethal necrosis disease and can transmit via infected maize seeds.

Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds

2011 ◽  
Vol 171 (1) ◽  
pp. 292-294 ◽  
Author(s):  
Yongjiang Zhang ◽  
Wenjun Zhao ◽  
Mingfu Li ◽  
Hongjun Chen ◽  
Shuifang Zhu ◽  
...  
Keyword(s):  
Real Time ◽  
Mottle Virus ◽  
Rt Pcr ◽  
Maize Seeds ◽  
Chlorotic Mottle Virus ◽  
Maize Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

2013 ◽  
Vol 14 (7) ◽  
pp. 555-562 ◽  
Author(s):  
Jian-xiang Wu ◽  
Qiang Wang ◽  
Huan Liu ◽  
Ya-juan Qian ◽  
Yan Xie ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Virus Detection ◽  
Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Maize Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals Impact of Fungal Endophyte Colonization of Maize (Zea mays L.) on Induced Resistance to Thrips- and Aphid-Transmitted Viruses

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 416 ◽  
Author(s):  
Simon Kiarie ◽  
Johnson O. Nyasani ◽  
Linnet S. Gohole ◽  
Nguya K. Maniania ◽  
Sevgan Subramanian
Keyword(s):  
Integrated Management ◽  
Management Strategies ◽  
Fungal Endophyte ◽  
Eastern Africa ◽  
Maize Seeds ◽  
Fungal Isolates ◽  
Chlorotic Mottle Virus ◽  
Maize Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Maize Plants

In eastern Africa, Maize lethal necrosis (MLN) is caused by the co-infection of maize plants with Maize chlorotic mottle virus (MCMV) (Tombusviridae: Machlomovirus) and Sugarcane mosaic virus (SCMV) (Potyviridae: Potyvirus). With the disease being new to Africa, minimal effective management strategies exist against it. This study examined the potential of 10 fungal isolates to colonize maize plants and induce resistance against MCMV and SCMV. Maize seeds were soaked in fungal inoculum, sown and evaluated for endophytic colonization. Fungus-treated plants were challenge-inoculated with SCMV and/or MCMV to assess the effects of fungal isolates on the viruses in terms of incidence, severity and virus titers over time. Isolates of Trichoderma harzianum, Trichoderma atroviride and Hypocrea lixii colonized different plant sections. All plants singly or dually-inoculated with SCMV and MCMV tested positive for the viruses by reverse transcription-polymerase chain reaction (RT-PCR). Maize plants inoculated by T. harzianum and Metarhizium. anisopliae resulted in up to 1.4 and 2.7-fold reduced SCMV severity and titer levels, respectively, over the controls but had no significant effect on MCMV. The results show that both T. harzianum and M. anisopliae are potential candidates for inducing resistance against SCMV and can be used for the integrated management of MLN.

Sign in / Sign up

Export Citation Format

Share Document