Comparative study of the inverse versus normal bicontinuous cubic phases of the β-d-glucopyranoside water-driven self-assemblies using fluorescent probes

RSC Advances ◽  
2016 ◽  
Vol 6 (1) ◽  
pp. 227-235 ◽  
Author(s):  
N. Idayu Zahid ◽  
Osama K. Abou-Zied ◽  
N. A. Nabila Saari ◽  
Rauzah Hashim
Keyword(s):  
Comparative Study ◽  
Fluorescent Probes ◽  
Water System ◽  
Head Group ◽  
Space Group ◽  
Cubic Phases ◽  
Bicontinuous Cubic

This work investigates the head group region of the inverse and normal bicontinuous cubic phases (Ia3d space group) of the glucopyranoside/water system using 2-(2′-hydroxyphenyl)benzoxazole and its derivatives as fluorescent probes.

scholarly journals Transgene expression in mice of the Opa1 mitochondrial transmembrane protein through bicontinuous cubic lipoplexes containing gemini imidazolium surfactants

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Mónica Muñoz-Úbeda ◽  
Martina Semenzato ◽  
Anais Franco-Romero ◽  
Elena Junquera ◽  
Emilio Aicart ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Transmembrane Protein ◽  
Quantitative Polymerase Chain Reaction ◽  
Genetic Material ◽  
Cationic Lipids ◽  
Head Group ◽  
Polar Head ◽  
Cubic Phases ◽  
Head Groups ◽  
Bicontinuous Cubic

Abstract Background Lipoplexes are non-viral vectors based on cationic lipids used to deliver DNA into cells, also known as lipofection. The positively charge of the hydrophilic head-group provides the cationic lipids the ability to condensate the negatively charged DNA into structured complexes. The polar head can carry a large variety of chemical groups including amines as well as guanidino or imidazole groups. In particular, gemini cationic lipids consist of two positive polar heads linked by a spacer with different length. As for the hydrophobic aliphatic chains, they can be unsaturated or saturated and are connected to the polar head-groups. Many other chemical components can be included in the formulation of lipoplexes to improve their transfection efficiency, which often relies on their structural features. Varying these components can drastically change the arrangement of DNA molecules within the lamellar, hexagonal or cubic phases that are provided by the lipid matrix. Lipofection is widely used to deliver genetic material in cell culture experiments but the simpler formulations exhibit major drawbacks related to low transfection, low specificity, low circulation half-life and toxicity when scaled up to in vivo experiments. Results So far, we have explored in cell cultures the transfection ability of lipoplexes based on gemini cationic lipids that consist of two C16 alkyl chains and two imidazolium polar head-groups linked with a polyoxyethylene spacer, (C16Im)2(C4O). Here, PEGylated lipids have been introduced to the lipoplex formulation and the transgene expression of the Opa1 mitochondrial transmembrane protein in mice was assessed. The addition of PEG on the surface of the lipid mixed resulted in the formation of Ia3d bicontinuous cubic phases as determined by small angle X-ray scattering. After a single intramuscular administration, the cubic lipoplexes were accumulated in tissues with tight endothelial barriers such as brain, heart, and lungs for at least 48 h. The transgene expression of Opa1 in those organs was identified by western blotting or RNA expression analysis through quantitative polymerase chain reaction. Conclusions The expression reported here is sufficient in magnitude, duration and toxicity to consolidate the bicontinuous cubic structures formed by (C16Im)2(C4O)-based lipoplexes as valuable therapeutic agents in the field of gene delivery. Graphical Abstract

scholarly journals Calculations of and Evidence for Chain Packing Stress in Inverse Lyotropic Bicontinuous Cubic Phases

Langmuir ◽  
2007 ◽  
Vol 23 (13) ◽  
pp. 7276-7285 ◽  
Author(s):  
Gemma C. Shearman ◽  
Bee J. Khoo ◽  
Mary-Lynn Motherwell ◽  
Kenneth A. Brakke ◽  
Oscar Ces ◽  
...  
Keyword(s):  
Chain Packing ◽  
Cubic Phases ◽  
Bicontinuous Cubic

scholarly journals Influence of a pH-sensitive polymer on the structure of monoolein cubosomes

Soft Matter ◽  
2017 ◽  
Vol 13 (41) ◽  
pp. 7571-7577 ◽  
Author(s):  
Monika Kluzek ◽  
Arwen I. I. Tyler ◽  
Shiqi Wang ◽  
Rongjun Chen ◽  
Carlos M. Marques ◽  
...  
Keyword(s):  
Ph Sensitive ◽  
Submicron Size ◽  
Cubic Phases ◽  
Bicontinuous Cubic ◽  
Ph Sensitive Polymer

Cubosomes consist in submicron size particles of lipid bicontinuous cubic phases stabilized by surfactant polymers.

scholarly journals Comparative study of the electrochemical properties of P4332 and Fd3m space group of LiNi0.45Cu0.05Mn1.5O4 cathode materials

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Miao-Miao Deng ◽  
Da-Wei Zhang ◽  
Aqsa Yasmin ◽  
Jia-Ying Liao ◽  
Xiao-Dong He ◽  
...  
Keyword(s):  
Comparative Study ◽  
Electrochemical Properties ◽  
Cathode Materials ◽  
Space Group

Plasma membrane fluidity in isolated rat hepatocytes: A comparative study using DPH and TMA-DPH as fluorescent probes

1989 ◽  
Vol 4 (3) ◽  
pp. 221-227 ◽  
Author(s):  
ANTONIO BENEDETTI ◽  
GIANNA FERRETTI ◽  
GIOVANNA CURATOLA ◽  
EUGENIO BRUNELLI ◽  
ANNE MARIE JÉZÉQUEL ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Comparative Study ◽  
Membrane Fluidity ◽  
Fluorescent Probes ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Plasma Membrane Fluidity ◽  
Isolated Rat
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