PSS-GN nanocomposites as highly-efficient peroxidase mimics and their applications in colorimetric detection of glucose in serum

RSC Advances ◽  
2015 ◽  
Vol 5 (110) ◽  
pp. 90400-90407 ◽  
Author(s):  
Jing Chen ◽  
Jia Ge ◽  
Lin Zhang ◽  
Zhaohui Li ◽  
Saisai Zhou ◽  
...  
Keyword(s):  
Colorimetric Detection ◽  
Serum Samples ◽  
Highly Efficient ◽  
Peroxidase Mimics

PSS-GN nanocomposites were firstly proposed as a peroxidase-like mimic successfully and utilized in the determination of glucose in serum samples.

scholarly journals Highly Efficient Gold Nano-Flower Optical Biosensor Doped in a Sol–Gel/PEG Matrix for the Determination of a Calcitonin Biomarker in Different Serum Samples

ACS Omega ◽  
2020 ◽  
Vol 5 (11) ◽  
pp. 5629-5637 ◽  
Author(s):  
Walaa E. Omer ◽  
Maged A. El-Kemary ◽  
Mostafa M. Elsaady ◽  
Mona N. Abou-Omar ◽  
Ahmed O. Youssef ◽  
...  
Keyword(s):  
Sol Gel ◽  
Optical Biosensor ◽  
Serum Samples ◽  
Highly Efficient

Colorimetric Detection of Hg(II) Sensor Based on Mos2 Nanosheets Acting as Peroxidase Mimics

2021 ◽  
Vol 37 (3) ◽  
pp. 679-682
Author(s):  
S. Kavitha ◽  
S. Mary Jelastin Kala ◽  
A. Anand Babu Christus
Keyword(s):  
Colorimetric Detection ◽  
High Sensitivity ◽  
Colorimetric Determination ◽  
X Ray Diffraction ◽  
Mos2 Nanosheets ◽  
Peroxidase Mimic ◽  
Sem Image ◽  
Peroxidase Mimics ◽  
Ft Ir

This paper presents colorimetric determination of Hg(II) based on MoS2 nanosheets with peroxidase mimics activity. The structure of the this sensor by the peroxidase mimic activity material of MoS2 nanosheets with TMB (Tetramethylbenzidine) solution, the colorimetric detection target of Hg(II) is determined by on-off mechanism using biomolecule of cysteine. The MoS2 nanosheets evaluated by X-ray diffraction, FT-IR and SEM image, confirms formation of a flower like structure. Our results shows that a simple colorimetric detection using peroxidase mimic mechanism can be used to MoS2 nanosheets and determine the Hg(II) in aqueous solution with high sensitivity (10 nM) comparable to those of other nanomaterials. The result suggests that MoS2 nanosheets is a promising new and simple colorimetric sensor for applications in environmental and biological applications.

Chiral FexCuySe nanoparticles as peroxidase mimics for colorimetric detection of 3, 4-dihydroxy-phenylalanine enantiomers

2021 ◽  
Author(s):  
Shuyang Hu ◽  
Qiuyan Shuai ◽  
Yulong Lin ◽  
Yan Fu ◽  
Meng Li
Keyword(s):  
Quality Control ◽  
Colorimetric Detection ◽  
Cost Effective ◽  
Drug Analysis ◽  
High Catalytic Activity ◽  
Chiral Ligand ◽  
Peroxidase Mimic ◽  
Disease Treatment ◽  
Peroxidase Mimics

Abstract L-3,4-dihydroxy-phenylalanine (L-dopa) is the most widely used drug in Parkinson's disease treatment. However, development of cost-effective and high-throughput sensors to accurate enantioselective discrimination of L-dopa and D-dopa remains challenging to date. Herein, on the basis of the peroxidase-mimic activity of chiral FexCuySe nanoparticles, we demonstrated a novel colorimetric sensor for determination of chiral dopa. The surface chiral ligand, L/D-histidine (L/D-His), endowed the nanozymes with enantioselectivity in catalyzing the oxidation of dopa enantiomers. According to the values of kcat/Km, the efficiency of L-His modified nanoparticle (L-FexCuySe NPs) towards L-dopa was 1.56 times higher than that of D-dopa. While, D-His can facilely reverse the preference of the nanozyme to D-dopa. On the basis of high catalytic activity and enantioselectivity of L-FexCuySe NPs in oxidation of L-dopa, the L-FexCuySe NPs based system can be utilized for detection of L-dopa. The linear ranges for L-dopa determination were 5 µM to 0.125 mM and 0.125 mM to 1 mM with a detection limit of 1.02 µM. Critically, the developed sensor has been successfully applied in the quality control of clinical used L-dopa tablets. Our work sheds light on developing simple and sensitive chiral nanomaterials-based sensors for drug analysis.

One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters as peroxidase mimics for colorimetric detection of thrombin

2014 ◽  
Vol 50 (86) ◽  
pp. 13103-13106 ◽  
Author(s):  
Cheng Zheng ◽  
Ai-Xian Zheng ◽  
Bo Liu ◽  
Xiao-Long Zhang ◽  
Yu He ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Colorimetric Detection ◽  
Protein Detection ◽  
High Sensitivity ◽  
One Pot ◽  
Highly Efficient ◽  
Bimetallic Nanoclusters ◽  
Peroxidase Mimics ◽  
Sensitivity And Selectivity ◽  
Colorimetric Aptasensor

One-pot synthesized DNA-templated Ag/Pt bimetallic nanoclusters possess highly-efficient peroxidase-like catalytic activity and are applied to design a colorimetric aptasensor for the protein detection with high sensitivity and selectivity.

The Dtermination of Paracetamol by HPLC Validation of the Method and Application on Serum Samples

2018 ◽  
Vol 69 (3) ◽  
pp. 627-631 ◽  
Author(s):  
Viorica Ohriac (Popa) ◽  
Diana Cimpoesu ◽  
Adrian Florin Spac ◽  
Paul Nedelea ◽  
Voichita Lazureanu ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Hplc Analysis ◽  
Emotional Experience ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Liquid Chromatograph ◽  
Mobile Phase Flow Rate ◽  
Quantification Limit

Pain is defined as a disagreeable sensory and emotional experience related to a tissue or potential lesion. Paracetamol (Acetaminophen) is the most used non-morphine analgesic. For the determination of paracetamol we developed and validated the high performance liquid chromatography (HPLC) analysis using a Dionex Ultimate 3000 liquid chromatograph equipped with a multidimensional detector. After determining the optimum conditions of analysis (80/20 water / acetonitrile mobile phase, flow rate 1.0 mL / min, detection wavelength 245 nm) we validated the method following the following parameters: linearity of response function, linearity of results, limit (LD = 0.66 mg / mL) and quantification limit (LQ = 2.00 mg / mL), and precision. The method of determining paracetamol by HPLC was applied to 30 samples of serum collected from patients who had pain and were treated with paracetamol.

About the concept of synthesis of high-performance detection and positioning systems in the areas of elements falling, separated from strategic and space missiles on the trajectories of their flight during tests and regular operation

2018 ◽  
pp. 10-21
Author(s):  
G. G. Vokin
Keyword(s):  
High Performance ◽  
Physical Nature ◽  
Positioning Systems ◽  
Physical Conditions ◽  
Highly Efficient

The article describes the approach and topical issues of synthesis of highly efficient transportable systems for search and determination of coordinates in the areas of elements falling, separated from the missiles, taking into account the dislocation of pre-empted areas of fall and physical conditions in their territories. The principles of these systems are based on the rational integration of traditional and non-traditional information sensors of different physical nature, which record the moments of landing of separated parts of the missiles.

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

scholarly journals Immobilization of Pseudomonas aeruginosa static biomass on eggshell powder for on-line preconcentration and determination of Cr (VI)

2020 ◽  
Vol 18 (1) ◽  
pp. 303-313 ◽  
Author(s):  
Aamir Rasheed ◽  
Tahseen Ghous ◽  
Sumaira Mumtaz ◽  
Muhammad Nadeem Zafar ◽  
Kalsoom Akhter ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Time Flow ◽  
Highly Sensitive ◽  
Glass Column ◽  
On Line ◽  
Preconcentration Time ◽  
Ultra Trace

AbstractIn the present work, a novel continuous flow system (CFS) is developed for the preconcentration and determination of Cr (VI) using Pseudomonas aeruginosa static biomass immobilized onto an effective and low-cost solid support of powdered eggshells. A mini glass column packed with the immobilized biosorbent is incorporated in a CFS for the preconcentration and determination of Cr (VI) from aqueous solutions. The method is based on preconcentration, washing and elution steps followed by colorimetric detection with 1,5-diphenyl carbazide in sulphuric acid. The effects of several variables such as pH, retention time, flow rate, eluent concentration and loaded volume are studied. Under optimal conditions, the CFS method has a linear range between 10 and 100 μg L-1 and a detection limit of 6.25 μg L-1 for the determination of Cr (VI). The sampling frequency is 10 samples per hour with a preconcentration time of 5 mins. Furthermore, after washing with a 0.1 M buffer (pH 3.0), the activity of the biosorbent is regenerated and remained comparable for more than 200 cycles. Scanning electron microscopy reveals a successful immobilization of biomass on eggshells powder and precipitation of Cr (VI) on the bacterial cell surface. The proposed method proves highly sensitive and could be suitable for the determination of Cr (VI) at an ultra-trace level.

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