Synthesis of hollow mesoporous ruthenium nanoparticles: evaluation of physico-chemical properties and toxicity

RSC Advances ◽  
2015 ◽  
Vol 5 (97) ◽  
pp. 79616-79623 ◽  
Author(s):  
Sakthivel Ramasamy ◽  
Devasier Bennet ◽  
Sanghyo Kim
Keyword(s):  
Structural Characterization ◽  
Biomedical Application ◽  
Chemical Properties ◽  
Template Method ◽  
Ruthenium Nanoparticles ◽  
Facile Synthesis ◽  
Physico Chemical

Facile synthesis of hollow mesoporous structured Ru-NPs by dual template method, structural characterization and in vitro biophysical and uptake evaluation for biomedical application.

scholarly journals Impact of Quantum Dot Surface on Complex Formation with Chlorin e6 and Photodynamic Therapy

Nanomaterials ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 9 ◽  
Author(s):  
Artiom Skripka ◽  
Dominyka Dapkute ◽  
Jurga Valanciunaite ◽  
Vitalijus Karabanovas ◽  
Ricardas Rotomskis
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Complex Formation ◽  
Chemical Properties ◽  
Disease Diagnosis ◽  
Chlorin E6 ◽  
Deep Tissue ◽  
Physico Chemical

Nanomaterials have permeated various fields of scientific research, including that of biomedicine, as alternatives for disease diagnosis and therapy. Among different structures, quantum dots (QDs) have distinctive physico-chemical properties sought after in cancer research and eradication. Within the context of cancer therapy, QDs serve the role of transporters and energy donors to photodynamic therapy (PDT) drugs, extending the applicability and efficiency of classic PDT. In contrast to conventional PDT agents, QDs’ surface can be designed to promote cellular targeting and internalization, while their spectral properties enable better light harvesting and deep-tissue use. Here, we investigate the possibility of complex formation between different amphiphilic coating bearing QDs and photosensitizer chlorin e6 (Ce6). We show that complex formation dynamics are dependent on the type of coating—phospholipids or amphiphilic polymers—as well as on the surface charge of QDs. Förster’s resonant energy transfer occurred in every complex studied, confirming the possibility of indirect Ce6 excitation. Nonetheless, in vitro PDT activity was restricted only to negative charge bearing QD-Ce6 complexes, correlating with better accumulation in cancer cells. Overall, these findings help to better design such and similar complexes, as gained insights can be straightforwardly translated to other types of nanostructures—expanding the palette of possible therapeutic agents for cancer therapy.

scholarly journals THE EFFECT OF IN VITRO EGG PLUMPING ON EGG WHITE PHYSICO-CHEMICAL PROPERTIES

1975 ◽  
Vol 24 (1) ◽  
pp. 171-172
Author(s):  
B. SAUVEUR ◽  
Mme Huguette ANTOINE
Keyword(s):  
Chemical Properties ◽  
Egg White ◽  
Physico Chemical

scholarly journals IN VITRO STUDY OF PHYSICO-CHEMICAL PROPERTIES OF THE «BALANCED SALT SOLUTION − H2O» SYSTEM WITH THE PURPOSE OF DEVELOPMENT OF TREATMENT, REHABILITATION AND PREVENTIVE MEASURES IN OPHTHALMOLOGY

2021 ◽  
Author(s):  
O. Kofanova ◽  
К. Tkachuk ◽  
O. Kofanov ◽  
M. Saveliev ◽  
O. Tverda ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Mathematical Models ◽  
Salt Solution ◽  
Dynamic Viscosity ◽  
Preventive Measures ◽  
Chemical Properties ◽  
In Vitro Study ◽  
Vitro Study ◽  
Physico Chemical

The research is devoted to the in vitro study of the behavior of the model system «irrigation balanced salt solution BSS − H2O» in order to further develop treatment, rehabilitation and prevention measures for patients with ophthalmic diseases. The aim of the investigation is the experimental determination and analysis of physico-chemical properties of irrigated balanced salt solution, the study of its behavior in the aquatic environment to model changes in the characteristics of intraocular fluid and further development of rehabilitation and preventive measures for ophthalmic patients. Materials and Methods. The study used methods of physico-chemical analysis, in particular, densimetry and viscosimetry of model systems containing BSS for intraocular use and double-distilled water in different volume ratios. The density of the system was determined pycnometrically (20.00 0С ± 0.05 0С; 101.3 kPa); the correction for weight loss of bodies in the air has been taken into account. Viscosity was measured under the same conditions with an Ostwald viscometer. Statistical analysis and evaluation of the reliability of the results were performed using such software products as MS Excel 2007, Google Spreadsheets, SPSS Statistics. Results. Approximate mathematical models of the dependences of density and kinematic and dynamic viscosity on the composition of the multicomponent system «BSS – H2O» were obtained with quite high coefficients of determination. Statistical significance and adequacy of model selection were tested by Student's criterion at a significance level of 5 %. To develop therapeutic, prophylactic and rehabilitation measures, mathematical models of kinematic and dynamic viscosity dependences on the density of the «BSS – H2O» system, measured under the same conditions, were built. The models also have high coefficients of determination. Conclusion. The in vitro physico-chemical analysis of the system «BSS – H2O», as well as the obtained approximate mathematical models can be used to predict possible changes in the characteristics of irrigated balanced salt solution during its long stay in the patient's eye.

scholarly journals PDE5 Inhibitors-Loaded Nanovesicles: Physico-Chemical Properties and In Vitro Antiproliferative Activity

Nanomaterials ◽  
2016 ◽  
Vol 6 (5) ◽  
pp. 92 ◽  
Author(s):  
Roberta De Rose ◽  
Maria Cristiano ◽  
Marilena Celano ◽  
Valentina Maggisano ◽  
Ada Vero ◽  
...  
Keyword(s):  
Antiproliferative Activity ◽  
Chemical Properties ◽  
Pde5 Inhibitors ◽  
Physico Chemical

P–250 Mineral oil with high viscosity improves the stability of pH and osmolality in the human in vitro culture system

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
E Mestres ◽  
Q Matia-Algué ◽  
A Villamar ◽  
M García-Jiménez ◽  
A Casals ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Culture System ◽  
Chemical Properties ◽  
High Viscosity ◽  
Mineral Oil ◽  
Culture Conditions ◽  
Physico Chemical ◽  
Temperature Loss ◽  
The Stability

Abstract Study question Do commercial mineral oil brands differ in their capacity to stabilize the human embryo culture system, and is this related to the oil’s viscosity? Summary answer While the oils’ viscosity only had minor effects on temperature maintenance, it showed a direct correlation with the stability of pH and osmolality during culture. What is known already Mineral oil is a key component of the in vitro embryo culture system, which stabilizes temperature, pH and osmolality of the media during culture. Its use has been implemented worldwide for several decades and many manufacturers currently produce and commercialize oil intended for human embryo culture. Unfortunately, oil remains as one of the less characterized products in the IVF laboratory due to a lack of standardized nomenclature, production and testing. With differing physico-chemical properties, such as viscosity, oils produced by various manufacturers could behave differently to the same culture conditions and, thus, its use may need to be adjusted accordingly. Study design, size, duration Viscosity was quantified in three high-viscosity (H-V) and three low-viscosity (L-V) oils with a viscosity-meter. The required time for media’s pH to equilibrate using each oil was studied, as well as its subsequent stability outside the incubator for 30min. In-drop temperature was assessed during 15min when taking a dish outside the incubator, and again when putting it back. Additionally, each oil’s capacity to avoid media evaporation was studied with daily osmolality measurements during 7 days. Participants/materials, setting, methods pH equilibration was measured with a continuous pHmeter (Log&Guard, Vitrolife) in 4-well dishes prepared with 600µl of medium and 500µl of oil. For the other experiments, 35mm dishes with 4ml of oil and 20µl media droplets were used. pH stability was assessed after 0, 15 and 30min outside the incubator with a blood-gas-analyzer (epoc,SiemensHelthineers). A fine-gauge thermocouple was used to measure in-drop temperature loss/recovery. Daily osmolality readings were taken with a vapor pressure osmometer (Vapro5600,Wescor). Main results and the role of chance The selected oil samples had a viscosity of 115, 111, 52, 22, 18, and 12cP. The medium’s pH took approximately 12h to completely equilibrate under H-V oils, while it took less than 4h in L-V. Similarly, the rise in pH after 30min on a heated stage outside of the incubator with room atmosphere was 0.03, 0.04, 0.06, 0.13, 0.17, and 0.26, respectively. Dishes were taken out of the incubator and placed on a heated surface. In the first five minutes, the in-drop temperature loss ranged between –0.22 and –0.13oC/min, with no significant differences observed between oil types. However, temperature plateaued at a significantly higher value in L-V oils (36.5oC), compared to H-V brands (36.25–36.1oC; p = 0.0005). By contrast, all samples followed a similar pattern when the dishes were returned to the benchtop incubator, with temperature taking around 7 minutes to completely recover. Some media evaporated in all oil groups during the 7-day culture in a dry benchtop incubator. The linear regression performed to compare the evaporation rate between groups showed a statistically significant correlation between oil viscosity and the rate of evaporation (p < 0.0001), with an osmolality rise ranging between +2.55mmol/kg/day in the most viscous oil and +6.29mmol/kg/day in the least viscous. Limitations, reasons for caution While the selected oils for this study represent a wide range of options in the market, future projects could widen this selection and include additional tests, such as optimized bioassays. Results may vary between centers, and thus each laboratory should test and optimize their culture system with their own settings. Wider implications of the findings: Different oil brands have shown differing physico-chemical properties that have a direct effect on the culture system and the stability of several culture conditions. These results may be of major importance to adapt the settings and methodologies followed in each IVF laboratory according to the type of oil being used. Trial registration number Not applicable

In-Vitro protein digestibility, physico-chemical properties and nutritional quality of sorghum-green gram cookies supplemented with mango powder

2021 ◽  
pp. 39
Author(s):  
Keyword(s):  
Nutritional Quality ◽  
Chemical Properties ◽  
Protein Digestibility ◽  
Vital Role ◽  
Green Gram ◽  
In Vitro Protein Digestibility ◽  
Physico Chemical ◽  
Thiamine Content ◽  
Vitro Protein

Ready to Eat (RTE) sorghum cookies were prepared by incorporating green gram flour at 10%, 20%, 30%, dried mango powder at 10% and evaluated for their physico-chemical and nutritional properties. Protein, fat, fiber and ash increased with increase in green gram flour substitution as carbohydrate content decreased significantly. Significant differences (p ≤ 0.05) in protein content were seen in cookies ranging from 9.52% to 13.60%. Fiber increased significantly from 9.40% to 10.90%. In vitro protein digestibility ranged from 67.75 ± 0.01% to 90.05 ± 0.10 %. Vitamins analysed increased with addition of green gram flour. Thiamine content ranged from 0.22±0.02 to 0.61±0.02 mg/100g, riboflavin from 0.09±0.00 to 1.39±0.04 mg/100g and ascorbic acid from 13.87±0.79 to 19.31±0.94 mg/100g. Value addition of under-utilized crops like sorghum and green grams can play a vital role in development of high nutritional quality RTE products.

Mining a Nanoparticle Dataset, Compiled Within the MODENA-COST Action

2021 ◽  
pp. 1706-1724
Author(s):  
Sabine Van Miert ◽  
Jan Creylman ◽  
Geert R. Verheyen
Keyword(s):  
Chemical Properties ◽  
In Vitro Testing ◽  
Cost Action ◽  
Robust Model ◽  
Physico Chemical ◽  
Classification And Regression ◽  
Toxic Potential ◽  
Sheer Number

Engineered nanomaterials (ENM) have new or enhanced physico-chemical properties compared to their micron-sized counterparts, but may also have an increased toxic potential. Animal and in vitro testing are typically employed to investigate the toxic effects of (nano)materials. The sheer number of ENMs and their physico-chemical parameters make it impossible to only use in vivo and in vitro testing, and modelling technologies are also deployed to find relationships between ENM parameters and toxicity. A heterogenous dataset containing information on 192 nanoparticle endpoints was compiled within the MODENA COST-Action consortium. Here, the available data was mined to identify relationships between nanoparticle properties and cell-death as measured with four cytotoxicity assays. ANOVA, collinearity analyses and classification and regression trees gave indications on potential relations between the NP-properties and toxicity, but could not deliver a robust model. More information and datapoints are necessary to build well-validated models.

scholarly journals In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1186
Author(s):  
Lívia da Costa Pereira ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
Adriana Terezinha Neves Novellino Alves ◽  
Rodrigo Figueiredo de Brito Resende ◽  
Marcelo José Pinheiro Guedes de Uzeda ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Chemical Properties ◽  
Chemical Characterization ◽  
Guided Bone Regeneration ◽  
Tissue Reaction ◽  
In Vivo Evaluation ◽  
Physico Chemical ◽  
Tissue Reactions

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration.

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