scholarly journals Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures

RSC Advances ◽  
2015 ◽  
Vol 5 (54) ◽  
pp. 43353-43360 ◽  
Author(s):  
D. Weinberg ◽  
C. F. Adams ◽  
D. M. Chari
Keyword(s):  
Stem Cells ◽  
Magnetic Nanoparticles ◽  
Magnetic Fields ◽  
Neural Stem Cells ◽  
Magnetic Nanoparticle ◽  
Cell Tracking ◽  
Suspension Cultures ◽  
Clinical Grade ◽  
Non Invasive ◽  
Invasive Cell

This is the first demonstration that oscillating magnetic fields safely promote the uptake of a clinical-grade magnetic nanoparticle (Lumirem/Ferumoxsil) into neural stem cells for non-invasive cell tracking capabilities.

scholarly journals Therapeutic Potential of Magnetic Nanoparticle-Based Human Adipose-Derived Stem Cells in a Mouse Model of Parkinson’s Disease

2021 ◽  
Vol 22 (2) ◽  
pp. 654
Author(s):  
Ka Young Kim ◽  
Keun-A Chang
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Magnetic Nanoparticles ◽  
Substantia Nigra ◽  
Mouse Model ◽  
Magnetic Nanoparticle ◽  
Imaging System ◽  
Therapeutic Potential ◽  
Adipose Derived Stem Cells

Parkinson’s disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Several treatments for PD have focused on the management of physical symptoms using dopaminergic agents. However, these treatments induce various adverse effects, including hallucinations and cognitive impairment, owing to non-targeted brain delivery, while alleviating motor symptoms. Furthermore, these therapies are not considered ultimate cures owing to limited brain self-repair and regeneration abilities. In the present study, we aimed to investigate the therapeutic potential of human adipose-derived stem cells (hASCs) using magnetic nanoparticles in a 6-hydroxydopamine (6-OHDA)-induced PD mouse model. We used the Maestro imaging system and magnetic resonance imaging (MRI) for in vivo tracking after transplantation of magnetic nanoparticle-loaded hASCs to the PD mouse model. The Maestro imaging system revealed strong hASCs signals in the brains of PD model mice. In particular, MRI revealed hASCs distribution in the substantia nigra of hASCs-injected PD mice. Behavioral evaluations, including apomorphine-induced rotation and rotarod performance, were significantly recovered in hASCs-injected 6-OHDA induced PD mice when compared with saline-treated counterparts. Herein, we investigated whether hASCs transplantation using magnetic nanoparticles recovered motor functions through targeted brain distribution in a 6-OHDA induced PD mice. These results indicate that magnetic nanoparticle-based hASCs transplantation could be a potential therapeutic strategy in PD.

scholarly journals Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

2016 ◽  
Vol 7 ◽  
pp. 926-936 ◽  
Author(s):  
Igor M Pongrac ◽  
Marina Dobrivojević ◽  
Lada Brkić Ahmed ◽  
Michal Babič ◽  
Miroslav Šlouf ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Cellular Uptake ◽  
Cell Tracking ◽  
Cellular Migration ◽  
Cell Labeling ◽  
Blue Staining ◽  
Maghemite Nanoparticles ◽  
Acetoxymethyl Ester

Background: Cell tracking is a powerful tool to understand cellular migration, dynamics, homing and function of stem cell transplants. Nanoparticles represent possible stem cell tracers, but they differ in cellular uptake and side effects. Their properties can be modified by coating with different biocompatible polymers. To test if a coating polymer, poly(L-lysine), can improve the biocompatibility of nanoparticles applied to neural stem cells, poly(L-lysine)-coated maghemite nanoparticles were prepared and characterized. We evaluated their cellular uptake, the mechanism of internalization, cytotoxicity, viability and proliferation of neural stem cells, and compared them to the commercially available dextran-coated nanomag®-D-spio nanoparticles. Results: Light microscopy of Prussian blue staining revealed a concentration-dependent intracellular uptake of iron oxide in neural stem cells. The methyl thiazolyl tetrazolium assay and the calcein acetoxymethyl ester/propidium iodide assay demonstrated that poly(L-lysine)-coated maghemite nanoparticles scored better than nanomag®-D-spio in cell labeling efficiency, viability and proliferation of neural stem cells. Cytochalasine D blocked the cellular uptake of nanoparticles indicating an actin-dependent process, such as macropinocytosis, to be the internalization mechanism for both nanoparticle types. Finally, immunocytochemistry analysis of neural stem cells after treatment with poly(L-lysine)-coated maghemite and nanomag®-D-spio nanoparticles showed that they preserve their identity as neural stem cells and their potential to differentiate into all three major neural cell types (neurons, astrocytes and oligodendrocytes). Conclusion: Improved biocompatibility and efficient cell labeling makes poly(L-lysine)-coated maghemite nanoparticles appropriate candidates for future neural stem cell in vivo tracking studies.

scholarly journals The Potential of Intrinsically Magnetic Mesenchymal Stem Cells for Tissue Engineering

2018 ◽  
Vol 19 (10) ◽  
pp. 3159 ◽  
Author(s):  
Fransiscus Kerans ◽  
Lisa Lungaro ◽  
Asim Azfer ◽  
Donald Salter
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Nanoparticles ◽  
Mammalian Cells ◽  
Cell Tracking ◽  
Magnetic Domain ◽  
Superparamagnetic Iron Oxide ◽  
In Vitro Assays

The magnetization of mesenchymal stem cells (MSC) has the potential to aid tissue engineering approaches by allowing tracking, targeting, and local retention of cells at the site of tissue damage. Commonly used methods for magnetizing cells include optimizing uptake and retention of superparamagnetic iron oxide nanoparticles (SPIONs). These appear to have minimal detrimental effects on the use of MSC function as assessed by in vitro assays. The cellular content of magnetic nanoparticles (MNPs) will, however, decrease with cell proliferation and the longer-term effects on MSC function are not entirely clear. An alternative approach to magnetizing MSCs involves genetic modification by transfection with one or more genes derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesizes single-magnetic domain crystals which are incorporated into magnetosomes. MSCs with either or mms6 and mmsF genes are followed by bio-assimilated synthesis of intracytoplasmic magnetic nanoparticles which can be imaged by magnetic resonance (MR) and which have no deleterious effects on MSC proliferation, migration, or differentiation. The stable transfection of magnetosome-associated genes in MSCs promotes assimilation of magnetic nanoparticle synthesis into mammalian cells with the potential to allow MR-based cell tracking and, through external or internal magnetic targeting approaches, enhanced site-specific retention of cells for tissue engineering.

scholarly journals Live-cell time-lapse imaging and single-cell tracking of in vitro cultured neural stem cells – Tools for analyzing dynamics of cell cycle, migration, and lineage selection

Methods ◽  
2018 ◽  
Vol 133 ◽  
pp. 81-90 ◽  
Author(s):  
Katja M. Piltti ◽  
Brian J. Cummings ◽  
Krystal Carta ◽  
Ayla Manughian-Peter ◽  
Colleen L. Worne ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Single Cell ◽  
Cell Tracking ◽  
Time Lapse ◽  
Live Cell ◽  
Time Lapse Imaging

Non-invasive cell-tracking methods for adoptive T cell therapies

2021 ◽  
Author(s):  
Jelter Van Hoeck ◽  
Christian Vanhove ◽  
Stefaan C. De Smedt ◽  
Koen Raemdonck
Keyword(s):  
T Cell ◽  
Cell Tracking ◽  
Cell Therapies ◽  
Non Invasive ◽  
Invasive Cell

scholarly journals Long-term MR cell tracking of neural stem cells grafted in immunocompetent versus immunodeficient mice reveals distinct differences in contrast between live and dead cells

2010 ◽  
Vol 65 (2) ◽  
pp. 564-574 ◽  
Author(s):  
Stacey Cromer Berman ◽  
Chulani Galpoththawela ◽  
Assaf A. Gilad ◽  
Jeff W. M. Bulte ◽  
Piotr Walczak
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cell Tracking ◽  
Immunodeficient Mice

The structural and organic magnetoresistance response of poly(9-vinyl carbazole) using low applied magnetic fields and magnetic nanoparticle addition

2017 ◽  
Vol 5 (15) ◽  
pp. 3779-3787 ◽  
Author(s):  
Mariano Romero ◽  
Ricardo Faccio ◽  
Milton A. Tumelero ◽  
André A. Pasa ◽  
Alvaro W. Mombrú
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Fields ◽  
Magnetic Nanoparticle ◽  
Structural Modifications ◽  
Organic Magnetoresistance

Evidence of PVK singlet-to-triplet and structural modifications are revealed with the addition of magnetic nanoparticles and low applied magnetic fields.

Non-invasive neural stem cells become invasive in vitro by combined FGF2 and BMP4 signaling

Development ◽  
2013 ◽  
Vol 140 (18) ◽  
pp. e1808-e1808
Author(s):  
M. H. M. Sailer ◽  
A. Gerber ◽  
C. Tostado ◽  
G. Hutter ◽  
D. Cordier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Non Invasive
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