Effect of PLGA/lecithin hybrid microspheres and β-tricalcium phosphate granules on the physicochemical properties, in vitro degradation and biocompatibility of calcium phosphate cement

RSC Advances ◽  
2015 ◽  
Vol 5 (59) ◽  
pp. 47749-47756 ◽  
Author(s):  
Tingting Wu ◽  
Haishan Shi ◽  
Jiandong Ye
Keyword(s):  
Calcium Phosphate ◽  
Physicochemical Properties ◽  
Calcium Phosphate Cement ◽  
Tricalcium Phosphate ◽  
In Vitro Degradation ◽  
Cell Affinity

CPC with beta-TCP granules and PLGA/Lec microspheres reveals better degradability and cell affinity along with proper physicochemical properties.

Effects of Stirring and Fluid Perfusion on the In Vitro Degradation of Calcium Phosphate Cement/PLGA Composites

2015 ◽  
Vol 21 (11) ◽  
pp. 1171-1177 ◽  
Author(s):  
Jie An ◽  
Sander C.G. Leeuwenburgh ◽  
Joop G.C. Wolke ◽  
John A. Jansen
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
In Vitro Degradation

In vitro degradation rate of apatitic calcium phosphate cement with incorporated PLGA microspheres

2011 ◽  
Vol 7 (9) ◽  
pp. 3459-3468 ◽  
Author(s):  
R.P. Félix Lanao ◽  
S.C.G. Leeuwenburgh ◽  
J.G.C. Wolke ◽  
J.A. Jansen
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Degradation Rate ◽  
Plga Microspheres ◽  
In Vitro Degradation

Effects of Mn-doping on the structure and in vitro degradation of β-tricalcium phosphate

2021 ◽  
Author(s):  
Junjian Che ◽  
Hubiao Wang ◽  
Yunhai Ma ◽  
Feipeng Cao ◽  
Guoqin Liu ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
In Vitro Degradation ◽  
Mn Doping

scholarly journals Enhanced Osteogenesis of Dental Pulp Stem Cells In Vitro Induced by Chitosan–PEG-Incorporated Calcium Phosphate Cement

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2252
Author(s):  
Jae Eun Kim ◽  
Sangbae Park ◽  
Woong-Sup Lee ◽  
Jinsub Han ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Zeta Potential ◽  
Mechanical Strength ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Ion Trapping ◽  
Alizarin Red

The use of bone graft materials is required for the treatment of bone defects damaged beyond the critical defect; therefore, injectable calcium phosphate cement (CPC) is actively used after surgery. The application of various polymers to improve injectability, mechanical strength, and biological function of injection-type CPC is encouraged. We previously developed a chitosan–PEG conjugate (CS/PEG) by a sulfur (VI) fluoride exchange reaction, and the resulting chitosan derivative showed high solubility at a neutral pH. We have demonstrated the CPC incorporated with a poly (ethylene glycol) (PEG)-grafted chitosan (CS/PEG) and developed CS/PEG CPC. The characterization of CS/PEG CPC was conducted using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The initial properties of CS/PEG CPCs, such as the pH, porosity, mechanical strength, zeta potential, and in vitro biocompatibility using the WST-1 assay, were also investigated. Moreover, osteocompatibility of CS/PEG CPCs was carried out via Alizarin Red S staining, immunocytochemistry, and Western blot analysis. CS/PEG CPC has enhanced mechanical strength compared to CPC, and the cohesion test also demonstrated in vivo stability. Furthermore, we determined whether CS/PEG CPC is a suitable candidate for promoting the osteogenic ability of Dental Pulp Stem Cells (DPSC). The elution of CS/PEG CPC entraps more calcium ion than CPC, as confirmed through the zeta potential test. Accordingly, the ion trapping effect of CS/PEG is considered to have played a role in promoting osteogenic differentiation of DPSCs. The results strongly suggested that CS/PEG could be used as suitable additives for improving osteogenic induction of bone substitute materials.

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