A new method to determine the new C-Met inhibitor “Cabozantinib” in dosage form and human plasma via micelle-enhanced spectrofluorimetry

RSC Advances ◽  
2015 ◽  
Vol 5 (51) ◽  
pp. 40484-40490 ◽  
Author(s):  
Hany W. Darwish ◽  
Ali S. Abdelhameed ◽  
Ahmed H. Bakheit ◽  
Amer M. Alanazi
Keyword(s):  
Human Plasma ◽  
Dosage Form ◽  
Pharmaceutical Formulation ◽  
New Method ◽  
Spiked Human Plasma ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Met Inhibitor

A highly sensitive and simple micelle-enhanced spectrofluorimetric method was developed for the determination of cabozantinib (CBZ) in its pharmaceutical formulation and spiked human plasma without any derivatization.

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

scholarly journals Highly Sensitive Micellar Enhanced Spectrofluorimetric Method for Determination of Mirtazapine in Tablets and Human Urine: Application to In Vitro Drug Release and Content Uniformity Test

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Hany W. Darwish ◽  
Ahmed H. Bakheit ◽  
Raed M. Alharbi
Keyword(s):  
Drug Release ◽  
Human Urine ◽  
Dosage Form ◽  
Content Uniformity ◽  
In Vitro Drug Release ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Spiked Human Urine

A highly sensitive and simple micelle enhanced spectrofluorimetric method was developed for assaying mirtazapine (MRZ) in REMERON® tablets and spiked human urine directly without the need of derivatizing agent. The basis of the current procedure is the examination of the relative fluorescence intensity (RFI) of MRZ in sodium lauryl sulphate (SLS) micellar medium. The RFI of MRZ in water was enhanced markedly on addition of SLS. The RFI was measured at 403 nm after excitation at 320 nm. The fluorescence-concentration relationship was linear over the range 1–500 ng/mL, with lower detection limit of 0.399 ng/mL. The proposed method was successfully applied to the determination of MRZ in dosage form and spiked human urine. Recovery percentages of MRZ utilizing the current method were99.05±1.83,98.37±1.96, and100.41±2.61% for pure powder, pharmaceutical dosage form, and spiked human urine, respectively. The application of the proposed method was extended to test content uniformity and the in vitro drug release of REMERON tablets, according to USP guidelines.

Specific and highly sensitive spectrofluorimetric method for determination of febuxostat in its tablets and real human plasma. Application to stability studies

RSC Advances ◽  
2016 ◽  
Vol 6 (77) ◽  
pp. 73432-73439 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Abdel-Maaboud I. Mohamed ◽  
Sayed M. Derayea ◽  
Mohamed A. Hammad ◽  
Abobakr A. Mohamed
Keyword(s):  
Human Plasma ◽  
Stability Studies ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive

A simple, rapid, specific and highly sensitive spectrofluorimetric method has been developed for determination of febuxostat (FEB) in its tablets and real human plasma.

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