scholarly journals Stability-indicating spectrofluorimetric method with enhanced sensitivity for determination of vancomycin hydrochloride in pharmaceuticals and spiked human plasma: Application to degradation kinetics

2018 ◽  
Vol 26 (2) ◽  
pp. 834-841 ◽  
Author(s):  
Mohie Khaled Sharaf El-Din ◽  
Fawzia Ibrahim ◽  
Asmaa Kamal El-Deen ◽  
Kuniyoshi Shimizu
Keyword(s):  
Human Plasma ◽  
Degradation Kinetics ◽  
Spiked Human Plasma ◽  
Stability Indicating ◽  
Vancomycin Hydrochloride ◽  
Enhanced Sensitivity ◽  
Spectrofluorimetric Method

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

A fast spectrofluorimetric method for determination of carbinoxamine maleate in the nano-molar range. Application to pharmaceutical preparations, biological fluids and stability studies

2018 ◽  
Vol 10 (31) ◽  
pp. 3851-3858 ◽  
Author(s):  
Fatma Ahmed Aly ◽  
Nahed EL-Enany ◽  
Heba Elmansi ◽  
Amany Nabil
Keyword(s):  
Human Plasma ◽  
Biological Fluids ◽  
Pharmaceutical Preparations ◽  
Pure Form ◽  
Stability Studies ◽  
Spiked Human Plasma ◽  
Spectrofluorimetric Method

Carbinoxamine maleate (CBX), which is a common ingredient of cold and cough treatment preparations, is determined by a sensitive, simple and convenient spectrofluorimetric method in its pure form, pharmaceutical preparations and spiked human plasma.

Development and validation of a stability-indicating spectrofluorimetric method for the determination of H1N1 antiviral drug (oseltamivir phosphate) in human plasma through the Hantzsch reaction

RSC Advances ◽  
2015 ◽  
Vol 5 (35) ◽  
pp. 27735-27742 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Sayed M. Derayea ◽  
Islam M. Mostafa
Keyword(s):  
Human Plasma ◽  
Antiviral Drug ◽  
Dosage Forms ◽  
Hantzsch Reaction ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Spectrofluorimetric Method ◽  
Development And Validation ◽  
Oseltamivir Phosphate

A simple and sensitive spectrofluorimetric method has been described and validated for the determination of oseltamivir phosphate (OSP) in its pure form and pharmaceutical dosage forms.

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