Label-free C-reactive protein SERS detection with silver nanoparticle aggregates

RSC Advances ◽  
2015 ◽  
Vol 5 (44) ◽  
pp. 34720-34729 ◽  
Author(s):  
Hyunmin Kim ◽  
Eunjoo Kim ◽  
Eunsook Choi ◽  
Chul Su Baek ◽  
Bokyung Song ◽  
...  
Keyword(s):  
Silver Nanoparticle ◽  
Qualitative Approach ◽  
Self Assembled Monolayers ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Assembled Monolayers ◽  
Nanoparticle Aggregates ◽  
Sers Detection ◽  
Self Assembled

In this work, we report a qualitative approach for detecting the adsorption of C-reactive protein on phosphocholine-terminated self-assembled monolayers without the use of any labels.

scholarly journals Microfluidic Impedance Biosensor Chips Using Sensing Layers Based on DNA-Based Self-Assembled Monolayers for Label-Free Detection of Proteins

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 80
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Significant Shift ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as bio-sensor for label-free detection of proteins by using the example of cardiac troponin I. Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a possible charge transfer through the sensing layer, in addition to analyte-specific binding by corresponding antibodies and reduction of nonspecific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody was covalently coupled on the SAM using carbodiimide chemistry. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible impedance changes, while application of a troponin I sample, 1 ng/mL, led to a significant shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of biomarkers, such as cardiac markers, with the newly developed microfluidic impedance biosensor chip.

Peptide Self-Assembled Monolayers for Label-Free and Unamplified Surface Plasmon Resonance Biosensing in Crude Cell Lysate

2009 ◽  
Vol 81 (16) ◽  
pp. 6779-6788 ◽  
Author(s):  
Olivier R. Bolduc ◽  
Christopher M. Clouthier ◽  
Joelle N. Pelletier ◽  
Jean-François Masson
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Self Assembled Monolayers ◽  
Label Free ◽  
Crude Cell Lysate ◽  
Cell Lysate ◽  
Assembled Monolayers ◽  
Self Assembled

scholarly journals Raman-encoded microbeads for spectral multiplexing with SERS detection

RSC Advances ◽  
2015 ◽  
Vol 5 (18) ◽  
pp. 13762-13767 ◽  
Author(s):  
Yuming Lai ◽  
Shuqing Sun ◽  
Tao He ◽  
Sebastian Schlücker ◽  
Yuling Wang
Keyword(s):  
Gold Nanoparticles ◽  
Self Assembled Monolayers ◽  
Polystyrene Beads ◽  
Assembled Monolayers ◽  
Sers Detection ◽  
Spectral Multiplexing ◽  
Self Assembled

Spectral multiplexing on polystyrene beads by SERS was demonstrated by encoding the silica-encapsulated self-assembled monolayers of Raman reporter-coated gold nanoparticles.

scholarly journals Microfluidic Impedance Biosensor Chip with DNA-Based Self-Assembled Monolayers for Label-Free Detection of Cardiac Biomarker Troponin I

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 38
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Glass Slide ◽  
Self Assembled Monolayers ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Pdms Microchannel ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as biosensor for the detection of cardiac troponin I (cTnI). Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. As impedimetric biosensors allow direct and label-free analyte detection, they are particularly suitable for fast biomarker detection. This is essential in the diagnosis of cardiac infarctions to enable an early treatment promoting a positive outcome. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a low initial impedance in addition to analyte-specific binding by corresponding antibodies and reduction of non-specific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody (anti-cTnI) was covalently coupled on the SAM using carbodiimide chemistry. The PDMS microchannel was bonded on the glass slide with the functionalized electrodes, and the completed microfluidic impedance biosensor chip was connected to the readout system. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible signal changes, while application of a troponin I sample, 1 ng/mL, led to a significant signal shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of cardiac markers with the newly developed impedance biosensor chip.

Electrochemical behavior of self-assembled monolayers based on functionalized oligothiophenes

1998 ◽  
Vol 95 (6) ◽  
pp. 1339-1342 ◽  
Author(s):  
R. Michalitsch ◽  
A. El Kassmi ◽  
P. Lang ◽  
A. Yassar ◽  
F. Garnier
Keyword(s):  
Electrochemical Behavior ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

Imaging of patterned self-assembled monolayers by scanning photoelectron microscopy

2003 ◽  
Vol 104 ◽  
pp. 459-462 ◽  
Author(s):  
R. Klauser ◽  
M. Zharnikov ◽  
I.-H. Hong ◽  
S.-C. Wang ◽  
A. Gölzhäuser ◽  
...  
Keyword(s):  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Scanning Photoelectron Microscopy ◽  
Self Assembled
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