Electrochemical studies of homogeneous self-assembled monolayers versus mixed self-assembled monolayers on gold electrode for “label free” detection of heart fatty acid binding protein

2012 ◽  
Vol 526 ◽  
pp. 143-149 ◽  
Author(s):  
Dana Stan ◽  
Carmen-Marinela Mihailescu ◽  
Rodica Iosub ◽  
Carmen Moldovan ◽  
Mihaela Savin ◽  
...  
Keyword(s):  
Gold Electrode ◽  
Fatty Acid Binding Protein ◽  
Self Assembled Monolayers ◽  
Electrochemical Studies ◽  
Label Free ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled

scholarly journals Microfluidic Impedance Biosensor Chips Using Sensing Layers Based on DNA-Based Self-Assembled Monolayers for Label-Free Detection of Proteins

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 80
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Significant Shift ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as bio-sensor for label-free detection of proteins by using the example of cardiac troponin I. Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a possible charge transfer through the sensing layer, in addition to analyte-specific binding by corresponding antibodies and reduction of nonspecific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody was covalently coupled on the SAM using carbodiimide chemistry. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible impedance changes, while application of a troponin I sample, 1 ng/mL, led to a significant shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of biomarkers, such as cardiac markers, with the newly developed microfluidic impedance biosensor chip.

scholarly journals Microfluidic Impedance Biosensor Chip with DNA-Based Self-Assembled Monolayers for Label-Free Detection of Cardiac Biomarker Troponin I

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 38
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Glass Slide ◽  
Self Assembled Monolayers ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Pdms Microchannel ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as biosensor for the detection of cardiac troponin I (cTnI). Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. As impedimetric biosensors allow direct and label-free analyte detection, they are particularly suitable for fast biomarker detection. This is essential in the diagnosis of cardiac infarctions to enable an early treatment promoting a positive outcome. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a low initial impedance in addition to analyte-specific binding by corresponding antibodies and reduction of non-specific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody (anti-cTnI) was covalently coupled on the SAM using carbodiimide chemistry. The PDMS microchannel was bonded on the glass slide with the functionalized electrodes, and the completed microfluidic impedance biosensor chip was connected to the readout system. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible signal changes, while application of a troponin I sample, 1 ng/mL, led to a significant signal shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of cardiac markers with the newly developed impedance biosensor chip.

pH-regulated release of dopamine from well-ordered self-assembled monolayers: Electrochemical studies

2013 ◽  
Vol 33 (8) ◽  
pp. 5095-5099 ◽  
Author(s):  
Sayed Habib Kazemi ◽  
Abdolhamid Alizadeh ◽  
Rahim Mohamadi ◽  
Mohammad Mahdi Khodaei ◽  
Davood Kordestani
Keyword(s):  
Self Assembled Monolayers ◽  
Electrochemical Studies ◽  
Assembled Monolayers ◽  
Self Assembled
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