Facile fabrication of glycopolymer-based iron oxide nanoparticles and their applications in the carbohydrate–lectin interaction and targeted cell imaging

2016 ◽  
Vol 7 (6) ◽  
pp. 1337-1344 ◽  
Author(s):  
Chen Shao ◽  
Xueming Li ◽  
Zhichao Pei ◽  
Dongdong Liu ◽  
Lin Wang ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Imaging ◽  
Oxide Nanoparticles ◽  
Facile Fabrication ◽  
Novel Method

A novel method for facile fabrication of glycopolymer-based iron oxide nanoparticles (GIONs) is developed.

High-Resolution Cellular MRI: Gadolinium and Iron Oxide Nanoparticles for in-Depth Dual-Cell Imaging of Engineered Tissue Constructs

ACS Nano ◽  
2013 ◽  
Vol 7 (9) ◽  
pp. 7500-7512 ◽  
Author(s):  
Riccardo Di Corato ◽  
Florence Gazeau ◽  
Catherine Le Visage ◽  
Delphine Fayol ◽  
Pierre Levitz ◽  
...  
Keyword(s):  
Iron Oxide ◽  
High Resolution ◽  
Iron Oxide Nanoparticles ◽  
Cell Imaging ◽  
Oxide Nanoparticles ◽  
Cellular Mri ◽  
Tissue Constructs ◽  
Engineered Tissue

A novel iron oxide nanoparticle-based method for the determination of the antioxidant capacity of rapeseed oils at various stages of the refining process

2015 ◽  
Vol 7 (11) ◽  
pp. 4650-4660 ◽  
Author(s):  
Aleksandra Szydłowska-Czerniak ◽  
Anna Łaszewska ◽  
Agnieszka Tułodziecka
Keyword(s):  
Iron Oxide ◽  
Antioxidant Capacity ◽  
Iron Oxide Nanoparticles ◽  
Refining Process ◽  
Iron Oxide Nanoparticle ◽  
Oxide Nanoparticles ◽  
Novel Method ◽  
Oxide Nanoparticle ◽  
Capacity Determination

A novel method for antioxidant capacity determination based on the antioxidant-mediated generation and growth of iron oxide nanoparticles was elaborated.

scholarly journals Surface Engineered Iron Oxide Nanoparticles Generated by Inert Gas Condensation for Biomedical Applications

2021 ◽  
Vol 8 (3) ◽  
pp. 38
Author(s):  
Aver Hemben ◽  
Iva Chianella ◽  
Glenn John Thomas Leighton
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Physical Vapor Deposition ◽  
Biomedical Applications ◽  
Inert Gas ◽  
Oxide Nanoparticles ◽  
Free Standing ◽  
Gas Condensation ◽  
Inert Gas Condensation ◽  
Novel Method

Despite the lifesaving medical discoveries of the last century, there is still an urgent need to improve the curative rate and reduce mortality in many fatal diseases such as cancer. One of the main requirements is to find new ways to deliver therapeutics/drugs more efficiently and only to affected tissues/organs. An exciting new technology is nanomaterials which are being widely investigated as potential nanocarriers to achieve localized drug delivery that would improve therapy and reduce adverse drug side effects. Among all the nanocarriers, iron oxide nanoparticles (IONPs) are one of the most promising as, thanks to their paramagnetic/superparamagnetic properties, they can be easily modified with chemical and biological functions and can be visualized inside the body by magnetic resonance imaging (MRI), while delivering the targeted therapy. Therefore, iron oxide nanoparticles were produced here with a novel method and their properties for potential applications in both diagnostics and therapeutics were investigated. The novel method involves production of free standing IONPs by inert gas condensation via the Mantis NanoGen Trio physical vapor deposition system. The IONPs were first sputtered and deposited on plasma cleaned, polyethylene glycol (PEG) coated silicon wafers. Surface modification of the cleaned wafer with PEG enabled deposition of free-standing IONPs, as once produced, the soft-landed IONPs were suspended by dissolution of the PEG layer in water. Transmission electron microscopic (TEM) characterization revealed free standing, iron oxide nanoparticles with size < 20 nm within a polymer matrix. The nanoparticles were analyzed also by Atomic Force Microscope (AFM), Dynamic Light Scattering (DLS) and NanoSight Nanoparticle Tacking Analysis (NTA). Therefore, our work confirms that inert gas condensation by the Mantis NanoGen Trio physical vapor deposition sputtering at room temperature can be successfully used as a scalable, reproducible process to prepare free-standing IONPs. The PEG- IONPs produced in this work do not require further purification and thanks to their tunable narrow size distribution have potential to be a powerful tool for biomedical applications.

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Ferrozine Assay for Simple and Cheap Iron Analysis of Silica-Coated Iron Oxide Nanoparticles

2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrofluoric Acid ◽  
Inductively Coupled Plasma ◽  
Silica Shell ◽  
Oxide Nanoparticles ◽  
Inductively Coupled ◽  
Digestion Process ◽  
Plasma Methods ◽  
Iron Analysis

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>

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