Real-time label-free quantitative fluorescence microscopy-based detection of ATP using a tunable fluorescent nano-aptasensor platform

Nanoscale ◽  
2015 ◽  
Vol 7 (46) ◽  
pp. 19663-19672 ◽  
Author(s):  
Sajal Shrivastava ◽  
Il-Yung Sohn ◽  
Young-Min Son ◽  
Won-Il Lee ◽  
Nae-Eung Lee
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Biological Samples ◽  
Quantitative Measurement ◽  
High Fidelity ◽  
Label Free ◽  
Quantitative Fluorescence

Although real-time label-free fluorescent aptasensors based on nanomaterials are increasingly recognized as a useful strategy for the detection of target biomolecules with high fidelity, the lack of an imaging-based quantitative measurement platform limits their implementation with biological samples.

Nanophotonics based label free detection mechanism for real-time monitoring of interleukin-6

Nanoscale ◽  
2020 ◽  
Vol 12 (16) ◽  
pp. 9194-9207 ◽  
Author(s):  
Munezza A. Khan ◽  
Mohammad Mujahid ◽  
Say Chye Joachim Loo ◽  
Vidya N. Chamundeswari
Keyword(s):  
Photonic Crystals ◽  
Real Time ◽  
Interleukin 6 ◽  
High Fidelity ◽  
Label Free ◽  
Real Time Monitoring ◽  
Detection Mechanism ◽  
Label Free Detection ◽  
Real Time Detection

Magneto-photonic crystals/MPCs are promising candidates for devising high-fidelity embedded biosensor systems which offer facile & real time detection of diagnostic proteins.

Visualization of the polymerization dynamics of individual 25nm-diameter microtubules in real time

1987 ◽  
Vol 45 ◽  
pp. 636-637
Author(s):  
E. D. Salmon ◽  
R. A. Walker ◽  
E. T. O'Brien ◽  
N. K. Pryer ◽  
W. A. Voter ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Motility ◽  
Real Time ◽  
Mitotic Spindle ◽  
Dynamic Instability ◽  
Great Majority ◽  
Living Cells ◽  
Rapid Turnover ◽  
Quantitative Fluorescence

Microtubules (Mts) of the interphase cytoplasmic Mt complex (CMTC) and the mitotic spindle are dynamic polymers assembled from a cellular pool of tubulin by end-dependent association-dissociation reactions (1,2). Mts have an intrinsic structural polarity, which orients the direction of cell motility. Mts also play a major role in the active translocation of vesicles and chromosomes. Quantitative fluorescence microscopy studies using fluorescently labeled tubulin microinjected into living cells have shown that the great majority of Mts in the CMTC and the spindle exchange rapidly with tubulins in the cellular pool (2,3). Dynamic instability, as proposed by Mitchison and Kirschner, provides the best explanation for this rapid turnover of microtubules (1,2).Three major temporal phases of Mt polymerization and three major abrupt transitions between these phases characterize the dynamic instability behavior of pure tubulin (Fig. 1).

Robust and high fidelity real-time hybrid substructuring

2021 ◽  
Vol 157 ◽  
pp. 107720
Author(s):  
Christina Insam ◽  
Arian Kist ◽  
Henri Schwalm ◽  
Daniel J. Rixen
Keyword(s):  
Real Time ◽  
High Fidelity

scholarly journals Real‐Time and Label‐Free Measurement of Deubiquitinase Activity with a MspA Nanopore

ChemBioChem ◽  
2021 ◽  
Author(s):  
Spencer A. Shorkey ◽  
Jiale Du ◽  
Ryan Pham ◽  
Eric R. Strieter ◽  
Min Chen
Keyword(s):  
Real Time ◽  
Label Free

scholarly journals Label-Free and Quantitative Dry Mass Monitoring for Single Cells during In Situ Culture

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1635
Author(s):  
Ya Su ◽  
Rongxin Fu ◽  
Wenli Du ◽  
Han Yang ◽  
Li Ma ◽  
...  
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Hela Cells ◽  
Quantitative Measurement ◽  
Single Cells ◽  
Dry Mass ◽  
Quantitative Detection ◽  
Label Free ◽  
Mass Sensitivity

Quantitative measurement of single cells can provide in-depth information about cell morphology and metabolism. However, current live-cell imaging techniques have a lack of quantitative detection ability. Herein, we proposed a label-free and quantitative multichannel wide-field interferometric imaging (MWII) technique with femtogram dry mass sensitivity to monitor single-cell metabolism long-term in situ culture. We demonstrated that MWII could reveal the intrinsic status of cells despite fluctuating culture conditions with 3.48 nm optical path difference sensitivity, 0.97 fg dry mass sensitivity and 2.4% average maximum relative change (maximum change/average) in dry mass. Utilizing the MWII system, different intrinsic cell growth characteristics of dry mass between HeLa cells and Human Cervical Epithelial Cells (HCerEpiC) were studied. The dry mass of HeLa cells consistently increased before the M phase, whereas that of HCerEpiC increased and then decreased. The maximum growth rate of HeLa cells was 11.7% higher than that of HCerEpiC. Furthermore, HeLa cells were treated with Gemcitabine to reveal the relationship between single-cell heterogeneity and chemotherapeutic efficacy. The results show that cells with higher nuclear dry mass and nuclear density standard deviations were more likely to survive the chemotherapy. In conclusion, MWII was presented as a technique for single-cell dry mass quantitative measurement, which had significant potential applications for cell growth dynamics research, cell subtype analysis, cell health characterization, medication guidance and adjuvant drug development.

scholarly journals Quantitative Fluorescence Microscopy on SARS-CoV-2

2021 ◽  
Vol 120 (3) ◽  
pp. 360a
Author(s):  
Rayna M. Addabbo ◽  
John Kohler ◽  
Isaac Angert ◽  
Yan Chen ◽  
Heather Hanson ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Quantitative Fluorescence

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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