Effect of intra-membrane C60 fullerenes on the modulus of elasticity and the mechanical resistance of gel and fluid lipid bilayers

Nanoscale ◽  
2015 ◽  
Vol 7 (40) ◽  
pp. 17102-17108 ◽  
Author(s):  
Jihan Zhou ◽  
Dehai Liang ◽  
Sonia Contera
Keyword(s):  
Light Scattering ◽  
Lipid Bilayers ◽  
Modulus Of Elasticity ◽  
Mechanical Resistance ◽  
Afm Imaging ◽  
Gel Phase ◽  
C60 Fullerenes

AFM imaging and nanoindentation complemented by FTIR and light scattering revealed the effect of intramembrane fullerene C60 on fluid and gel phase lipid bilayers.

Fluid or gel phase lipid bilayers to study peptide-membrane interactions?

1996 ◽  
Vol 25 (1) ◽  
pp. 55-59 ◽  
Author(s):  
T. Pott ◽  
J. Dufourcq ◽  
E. J. Dufourc
Keyword(s):  
Lipid Bilayers ◽  
Membrane Interactions ◽  
Gel Phase

scholarly journals Variation of thermal conductivity of DPPC lipid bilayer membranes around the phase transition temperature

2017 ◽  
Vol 14 (130) ◽  
pp. 20170127 ◽  
Author(s):  
Sina Youssefian ◽  
Nima Rahbar ◽  
Christopher R. Lambert ◽  
Steven Van Dessel
Keyword(s):  
Phase Transition ◽  
Thermal Conductivity ◽  
Thermal Properties ◽  
Transition Temperature ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Phase Transition Temperature ◽  
Temperature Gradients ◽  
Phase Behaviour ◽  
Gel Phase

Given their amphiphilic nature and chemical structure, phospholipids exhibit a strong thermotropic and lyotropic phase behaviour in an aqueous environment. Around the phase transition temperature, phospholipids transform from a gel-like state to a fluid crystalline structure. In this transition, many key characteristics of the lipid bilayers such as structure and thermal properties alter. In this study, we employed atomistic simulation techniques to study the structure and underlying mechanisms of heat transfer in dipalmitoylphosphatidylcholine (DPPC) lipid bilayers around the fluid–gel phase transformation. To investigate this phenomenon, we performed non-equilibrium molecular dynamics simulations for a range of different temperature gradients. The results show that the thermal properties of the DPPC bilayer are highly dependent on the temperature gradient. Higher temperature gradients cause an increase in the thermal conductivity of the DPPC lipid bilayer. We also found that the thermal conductivity of DPPC is lowest at the transition temperature whereby one lipid leaflet is in the gel phase and the other is in the liquid crystalline phase. This is essentially related to a growth in thermal resistance between the two leaflets of lipid at the transition temperature. These results provide significant new insights into developing new thermal insulation for engineering applications.

scholarly journals Structural Correlations and Thermodynamics of the Gel Phase in Lipid Bilayers

2012 ◽  
Vol 102 (3) ◽  
pp. 503a
Author(s):  
Richard O. Tjörnhammar ◽  
Olle Edholm
Keyword(s):  
Lipid Bilayers ◽  
Gel Phase

Robust T-cell signaling triggered on soft polydimethylsiloxane-supported lipid-bilayers

2020 ◽  
Author(s):  
Anna H. Lippert ◽  
Ivan B. Dimov ◽  
Alexander Winkel ◽  
James McColl ◽  
Jane Humphrey ◽  
...  
Keyword(s):  
T Cell ◽  
Lipid Bilayers ◽  
T Cell Activation ◽  
Cell Activation ◽  
Tyrosine Phosphatase ◽  
Supported Lipid Bilayers ◽  
Cell Contact ◽  
Mechanical Resistance ◽  
Cell Contacts ◽  
Cell Cell

AbstractThe T-cell receptor (TCR) is thought to be triggered either by mechano-transduction or local tyrosine phosphatase exclusion at cell-cell contacts. However, the effects of the mechanical properties of activating surfaces have only been tested for late-stage T-cell activation, and phosphatase segregation has mostly been studied on glass-supported lipid bilayers that favor imaging but are orders-of-magnitude stiffer than typical cells. We developed a method for attaching lipid bilayers to polydimethylsiloxane polymer supports, producing ‘soft bilayers’ with physiological levels of mechanical resistance (Young’s modulus of 4 kPa). Comparisons of T-cell behavior on soft and glass-supported bilayers revealed that early calcium signaling is unaffected by substrate rigidity, implying that early steps in TCR triggering are not mechanosensitive. Robust phosphatase exclusion was observed on the soft bilayers, however, suggesting it likely occurs at cell-cell contacts. This work sets the stage for an imaging-based exploration of receptor signaling under conditions closely mimicking physiological cell-cell contact.

scholarly journals AFM nanoindentation reveals decrease of elastic modulus of lipid bilayers near freezing point of water

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Calum Gabbutt ◽  
Wuyi Shen ◽  
Jacob Seifert ◽  
Sonia Contera
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Structural Changes ◽  
Freezing Point ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging ◽  
Underlying Causes ◽  
Irreversible Injury

AbstractCell lipid membranes are the primary site of irreversible injury during freezing/thawing and cryopreservation of cells, but the underlying causes remain unknown. Here, we probe the effect of cooling from 20 °C to 0 °C on the structure and mechanical properties of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers using atomic force microscopy (AFM) imaging and AFM-based nanoindentation in a liquid environment. The Young’s modulus of elasticity (E) at each temperature for DPPC was obtained at different ionic strengths. Both at 20 mM and 150 mM NaCl, E of DPPC bilayers increases exponentially –as expected–as the temperature is lowered between 20 °C and 5 °C, but at 0 °C E drops from the values measured at 5 °C. Our results support the hypothesis that mechanical weakening of the bilayer at 0 °C  is produced by  structural changes at the lipid-fluid interface.

scholarly journals Preferential accumulation of Aβ(1−42) on gel phase domains of lipid bilayers: An AFM and fluorescence study

2007 ◽  
Vol 1768 (1) ◽  
pp. 146-154 ◽  
Author(s):  
A. Choucair ◽  
M. Chakrapani ◽  
B. Chakravarthy ◽  
J. Katsaras ◽  
L.J. Johnston
Keyword(s):  
Lipid Bilayers ◽  
Fluorescence Study ◽  
Preferential Accumulation ◽  
Gel Phase

scholarly journals Lipid Bilayers in the Gel Phase Become Saturated by Triton X-100 at Lower Surfactant Concentrations Than Those in the Fluid Phase

2012 ◽  
Vol 102 (11) ◽  
pp. 2510-2516 ◽  
Author(s):  
Hasna Ahyayauch ◽  
M. Isabel Collado ◽  
Alicia Alonso ◽  
Felix M. Goñi
Keyword(s):  
Lipid Bilayers ◽  
Fluid Phase ◽  
Gel Phase ◽  
Triton X

scholarly journals Viscoelastic and light scattering studies on thermally induced sol to gel phase transition in fish myosin solutions

Biopolymers ◽  
2003 ◽  
Vol 69 (4) ◽  
pp. 498-507 ◽  
Author(s):  
Sawa Kouchi ◽  
Shingo Kondo ◽  
Kazuko Ooi ◽  
Hisashi Ichikawa ◽  
Toshiaki Dobashi
Keyword(s):  
Phase Transition ◽  
Light Scattering ◽  
Thermally Induced ◽  
Gel Phase

Fish Antifreeze Glycoproteins Protect Cellular Membranes During Lipid-Phase Transitions

Physiology ◽  
1997 ◽  
Vol 12 (4) ◽  
pp. 189-194
Author(s):  
LM Hays ◽  
RE Feeney ◽  
F Tablin ◽  
AE Oliver ◽  
NJ Walker ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Liquid Crystalline ◽  
Antarctic Fish ◽  
Cell Types ◽  
Lipid Phase ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Antifreeze Glycoproteins ◽  
Lipid Phase Transitions ◽  
Gel Phase

Antifreeze proteins from Antarctic fish depress solution freezing temperatures, inhibit ice crystal formation, and prevent recrystallization on rewarming. They have been used to enhance survival of some cell types during hypothermic storage. The mechanism of their protection is thought to be important during the transition of lipid bilayers from a liquid crystalline to a gel phase.

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