Fluid or gel phase lipid bilayers to study peptide-membrane interactions?

1996 ◽  
Vol 25 (1) ◽  
pp. 55-59 ◽  
Author(s):  
T. Pott ◽  
J. Dufourcq ◽  
E. J. Dufourc
Keyword(s):  
Lipid Bilayers ◽  
Membrane Interactions ◽  
Gel Phase

scholarly journals Variation of thermal conductivity of DPPC lipid bilayer membranes around the phase transition temperature

2017 ◽  
Vol 14 (130) ◽  
pp. 20170127 ◽  
Author(s):  
Sina Youssefian ◽  
Nima Rahbar ◽  
Christopher R. Lambert ◽  
Steven Van Dessel
Keyword(s):  
Phase Transition ◽  
Thermal Conductivity ◽  
Thermal Properties ◽  
Transition Temperature ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Phase Transition Temperature ◽  
Temperature Gradients ◽  
Phase Behaviour ◽  
Gel Phase

Given their amphiphilic nature and chemical structure, phospholipids exhibit a strong thermotropic and lyotropic phase behaviour in an aqueous environment. Around the phase transition temperature, phospholipids transform from a gel-like state to a fluid crystalline structure. In this transition, many key characteristics of the lipid bilayers such as structure and thermal properties alter. In this study, we employed atomistic simulation techniques to study the structure and underlying mechanisms of heat transfer in dipalmitoylphosphatidylcholine (DPPC) lipid bilayers around the fluid–gel phase transformation. To investigate this phenomenon, we performed non-equilibrium molecular dynamics simulations for a range of different temperature gradients. The results show that the thermal properties of the DPPC bilayer are highly dependent on the temperature gradient. Higher temperature gradients cause an increase in the thermal conductivity of the DPPC lipid bilayer. We also found that the thermal conductivity of DPPC is lowest at the transition temperature whereby one lipid leaflet is in the gel phase and the other is in the liquid crystalline phase. This is essentially related to a growth in thermal resistance between the two leaflets of lipid at the transition temperature. These results provide significant new insights into developing new thermal insulation for engineering applications.

scholarly journals Structural Correlations and Thermodynamics of the Gel Phase in Lipid Bilayers

2012 ◽  
Vol 102 (3) ◽  
pp. 503a
Author(s):  
Richard O. Tjörnhammar ◽  
Olle Edholm
Keyword(s):  
Lipid Bilayers ◽  
Gel Phase

scholarly journals Vaccinia Virus Interactions with the Cell Membrane Studied by New Chromatic Vesicle and Cell Sensor Assays

2006 ◽  
Vol 81 (3) ◽  
pp. 1140-1147 ◽  
Author(s):  
Z. Orynbayeva ◽  
S. Kolusheva ◽  
N. Groysman ◽  
N. Gavrielov ◽  
L. Lobel ◽  
...  
Keyword(s):  
Cell Surface ◽  
Vaccinia Virus ◽  
Lipid Bilayers ◽  
Growth Factor Receptor ◽  
Viral Envelope ◽  
Virus Infectivity ◽  
Membrane Interactions ◽  
Viral Interactions ◽  
Epidermal Growth ◽  
Virus Interactions

ABSTRACT The potential danger of cross-species viral infection points to the significance of understanding the contributions of nonspecific membrane interactions with the viral envelope compared to receptor-mediated uptake as a factor in virus internalization and infection. We present a detailed investigation of the interactions of vaccinia virus particles with lipid bilayers and with epithelial cell membranes using newly developed chromatic biomimetic membrane assays. This analytical platform comprises vesicular particles containing lipids interspersed within reporter polymer units that emit intense fluorescence following viral interactions with the lipid domains. The chromatic vesicles were employed as membrane models in cell-free solutions and were also incorporated into the membranes of epithelial cells, thereby functioning as localized membrane sensors on the cell surface. These experiments provide important insight into membrane interactions with and fusion of virions and the kinetic profiles of these processes. In particular, the data emphasize the significance of cholesterol/sphingomyelin domains (lipid rafts) as a crucial factor promoting bilayer insertion of the viral particles. Our analysis of virus interactions with polymer-labeled living cells exposed the significant role of the epidermal growth factor receptor in vaccinia virus infectivity; however, the data also demonstrated the existence of additional non-receptor-mediated mechanisms contributing to attachment of the virus to the cell surface and its internalization.

Distinct Helix Propensities and Membrane Interactions of Human and Rat IAPP1–19 Monomers in Anionic Lipid Bilayers

2015 ◽  
Vol 119 (8) ◽  
pp. 3366-3376 ◽  
Author(s):  
Cong Guo ◽  
Sébastien Côté ◽  
Normand Mousseau ◽  
Guanghong Wei
Keyword(s):  
Lipid Bilayers ◽  
Membrane Interactions ◽  
Anionic Lipid

scholarly journals Preferential accumulation of Aβ(1−42) on gel phase domains of lipid bilayers: An AFM and fluorescence study

2007 ◽  
Vol 1768 (1) ◽  
pp. 146-154 ◽  
Author(s):  
A. Choucair ◽  
M. Chakrapani ◽  
B. Chakravarthy ◽  
J. Katsaras ◽  
L.J. Johnston
Keyword(s):  
Lipid Bilayers ◽  
Fluorescence Study ◽  
Preferential Accumulation ◽  
Gel Phase

scholarly journals Lipid Bilayers in the Gel Phase Become Saturated by Triton X-100 at Lower Surfactant Concentrations Than Those in the Fluid Phase

2012 ◽  
Vol 102 (11) ◽  
pp. 2510-2516 ◽  
Author(s):  
Hasna Ahyayauch ◽  
M. Isabel Collado ◽  
Alicia Alonso ◽  
Felix M. Goñi
Keyword(s):  
Lipid Bilayers ◽  
Fluid Phase ◽  
Gel Phase ◽  
Triton X

Fish Antifreeze Glycoproteins Protect Cellular Membranes During Lipid-Phase Transitions

Physiology ◽  
1997 ◽  
Vol 12 (4) ◽  
pp. 189-194
Author(s):  
LM Hays ◽  
RE Feeney ◽  
F Tablin ◽  
AE Oliver ◽  
NJ Walker ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Liquid Crystalline ◽  
Antarctic Fish ◽  
Cell Types ◽  
Lipid Phase ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Antifreeze Glycoproteins ◽  
Lipid Phase Transitions ◽  
Gel Phase

Antifreeze proteins from Antarctic fish depress solution freezing temperatures, inhibit ice crystal formation, and prevent recrystallization on rewarming. They have been used to enhance survival of some cell types during hypothermic storage. The mechanism of their protection is thought to be important during the transition of lipid bilayers from a liquid crystalline to a gel phase.

scholarly journals Structural basis of membrane disruption and cellular toxicity by α-synuclein oligomers

Science ◽  
2017 ◽  
Vol 358 (6369) ◽  
pp. 1440-1443 ◽  
Author(s):  
Giuliana Fusco ◽  
Serene W. Chen ◽  
Philip T. F. Williamson ◽  
Roberta Cascella ◽  
Michele Perni ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Neurodegenerative Disorders ◽  
Cortical Neurons ◽  
Neuroblastoma Cells ◽  
Structural Basis ◽  
Membrane Disruption ◽  
Membrane Interactions ◽  
Primary Cortical Neurons ◽  
Cellular Toxicity ◽  
Structural Methods

Oligomeric species populated during the aggregation process of α-synuclein have been linked to neuronal impairment in Parkinson’s disease and related neurodegenerative disorders. By using solution and solid-state nuclear magnetic resonance techniques in conjunction with other structural methods, we identified the fundamental characteristics that enable toxic α-synuclein oligomers to perturb biological membranes and disrupt cellular function; these include a highly lipophilic element that promotes strong membrane interactions and a structured region that inserts into lipid bilayers and disrupts their integrity. In support of these conclusions, mutations that target the region that promotes strong membrane interactions by α-synuclein oligomers suppressed their toxicity in neuroblastoma cells and primary cortical neurons.

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