Degradable polymeric nanoparticles by aggregation of thermoresponsive polymers and “click” chemistry

Nanoscale ◽  
2015 ◽  
Vol 7 (40) ◽  
pp. 16823-16833 ◽  
Author(s):  
Andrzej Dworak ◽  
Daria Lipowska ◽  
Dawid Szweda ◽  
Jerzy Suwinski ◽  
Barbara Trzebicka ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Polymeric Nanoparticles ◽  
Thermoresponsive Polymers ◽  
Novel Approach

This study describes a novel approach to the preparation of crosslinked polymeric nanoparticles of controlled sizes that can be degraded under basic conditions.

Tuning of Polymeric Nanoparticles by Coassembly of Thermoresponsive Polymers and a Double Hydrophilic Thermoresponsive Block Copolymer

2016 ◽  
Vol 120 (20) ◽  
pp. 4635-4643 ◽  
Author(s):  
Qilu Zhang ◽  
Lenny Voorhaar ◽  
Sergey K. Filippov ◽  
Berin Fatma Yeşil ◽  
Richard Hoogenboom
Keyword(s):  
Block Copolymer ◽  
Polymeric Nanoparticles ◽  
Thermoresponsive Polymers

Novel approach for labeling of biopolymers with DOTA complexes using in situ click chemistry for quantification

Talanta ◽  
2015 ◽  
Vol 134 ◽  
pp. 468-475 ◽  
Author(s):  
Yide He ◽  
Diego Esteban-Fernández ◽  
Michael W. Linscheid
Keyword(s):  
Click Chemistry ◽  
Novel Approach

scholarly journals Fabrication and in vitro characterization of polymeric nanoparticles for Parkinson's therapy: a novel approach

2014 ◽  
Vol 50 (4) ◽  
pp. 869-876 ◽  
Author(s):  
Neha Gulati ◽  
Upendra Nagaich ◽  
Shubhini Saraf
Keyword(s):  
Drug Release ◽  
Polymeric Nanoparticles ◽  
Release Kinetics ◽  
Mode Of Delivery ◽  
Chitosan Nanoparticles ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Fickian Diffusion ◽  
Novel Approach

The objective of the research was to formulate and evaluate selegiline hydrochloride loaded chitosan nanoparticles for the Parkinson's therapy in order to improve its therapeutic effect and reducing dosing frequency. Taguchi method of design of experiments (L9 orthogonal array) was used to get optimized formulation. The selegiline hydrochloride loaded chitosan nanoparticles (SHPs) were prepared by ionic gelation of chitosan with tripolyphosphate anions (TPP) and tween 80 as surfactant. The SHPs had a mean size of (303.39 ± 2.01) nm, a zeta potential of +32.50mV, and entrapment efficiency of SHPs was 86.200 ± 1.38%. The in vitro drug release of SHPs was evaluated in phosphate buffer saline (pH 5.5) using goat nasal mucosa and found to be 82.529% ± 1.308 up to 28 h. Release kinetics studies showed that the release of drug from nanoparticles was anomalous (non-fickian) diffusion indicating the drug release is controlled by more than one process i.e. superposition of both phenomenon, the diffusion controlled as well as swelling controlled release. SHPs showed good stability results as found during stability studies at different temperatures as mentioned in ICH guidelines. The results revealed that selegiline hydrochloride loaded chitosan nanoparticles are most suitable mode of delivery of drug for promising therapeutic action.

scholarly journals Poly(A)-ClickSeq: click-chemistry for next-generation 3´-end sequencing without RNA enrichment or fragmentation

2017 ◽  
Author(s):  
Andrew Routh ◽  
Ping Ji ◽  
Elizabeth Jaworski ◽  
Zheng Xia ◽  
Wei Li ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Simple Procedure ◽  
Alternative Polyadenylation ◽  
Rna Seq ◽  
Cdna Synthesis ◽  
Novel Approach ◽  
Genome Wide ◽  
Recent Emergence ◽  
Complex Sample ◽  
A Site

AbstractThe recent emergence of alternative polyadenylation (APA) as an engine driving transcriptomic diversity has stimulated the development of sequencing methodologies designed to assess genome-wide polyadenylation events. The goal of these approaches is to enrich, partition, capture, and ultimately sequence poly(A) site junctions. However, these methods often require poly(A) enrichment, 3´ linker ligation steps, and RNA fragmentation, which can necessitate higher levels of starting RNA, increase experimental error, and potentially introduce bias. We recently reported a click-chemistry based method for generating RNAseq libraries called “ClickSeq”. Here, we adapt this method to direct the cDNA synthesis specifically toward the 3´ UTR/poly(A) tail junction of cellular RNA. With this novel approach, we demonstrate sensitive and specific enrichment for poly(A) site junctions without the need for complex sample preparation, fragmentation or purification. Poly(A)-ClickSeq (PAC-seq) is therefore a simple procedure that generates high-quality RNA-seq poly(A) libraries. As a proof-of-principle, we utilized PAC-seq to explore the poly(A) landscape of both human and Drosophila cells in culture and observed outstanding overlap with existing poly(A) databases and also identified previously unannotated poly(A) sites. Moreover, we utilize PAC-seq to quantify and analyze APA events regulated by CFIm25 illustrating how this technology can be harnessed to identify alternatively polyadenylated RNA.

scholarly journals The Antibiofilm Nanosystems for Improved Infection Inhibition of Microbes in Skin

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6392
Author(s):  
Yin-Ku Lin ◽  
Shih-Chun Yang ◽  
Ching-Yun Hsu ◽  
Jui-Tai Sung ◽  
Jia-You Fang
Keyword(s):  
Polymeric Nanoparticles ◽  
Close Contact ◽  
Drug Stability ◽  
Drug Loading ◽  
Metal Oxide Nanoparticles ◽  
Skin Infections ◽  
Novel Approach ◽  
The Matrix ◽  
Infection Inhibition

Biofilm formation is an important virulence factor for the opportunistic microorganisms that elicit skin infections. The recalcitrant feature of biofilms and their antibiotic tolerance impose a great challenge on the use of conventional therapies. Most antibacterial agents have difficulty penetrating the matrix produced by a biofilm. One novel approach to address these concerns is to prevent or inhibit the formation of biofilms using nanoparticles. The advantages of using nanosystems for antibiofilm applications include high drug loading efficiency, sustained or prolonged drug release, increased drug stability, improved bioavailability, close contact with bacteria, and enhanced accumulation or targeting to biomasses. Topically applied nanoparticles can act as a strategy for enhancing antibiotic delivery into the skin. Various types of nanoparticles, including metal oxide nanoparticles, polymeric nanoparticles, liposomes, and lipid-based nanoparticles, have been employed for topical delivery to treat biofilm infections on the skin. Moreover, nanoparticles can be designed to combine with external stimuli to produce magnetic, photothermal, or photodynamic effects to ablate the biofilm matrix. This study focuses on advanced antibiofilm approaches based on nanomedicine for treating skin infections. We provide in-depth descriptions on how the nanoparticles could effectively eliminate biofilms and any pathogens inside them. We then describe cases of using nanoparticles for antibiofilm treatment of the skin. Most of the studies included in this review were supported by in vivo animal infection models. This article offers an overview of the benefits of nanosystems for treating biofilms grown on the skin.

scholarly journals Electrospray-based synthesis of fluorescent poly(d,l-lactide-co-glycolide) nanoparticles for the efficient delivery of an anticancer drug and self-monitoring its effect in drug-resistant breast cancer cells

2020 ◽  
Vol 1 (8) ◽  
pp. 3033-3048
Author(s):  
Manosree Chatterjee ◽  
Ritwik Maity ◽  
Souvik Das ◽  
Nibedita Mahata ◽  
Biswarup Basu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Polymeric Nanoparticles ◽  
Metastatic Breast ◽  
Drug Resistant ◽  
Blue Fluorescence ◽  
Self Monitoring ◽  
Novel Approach ◽  
Efficient Delivery

A novel approach used to synthesize antimetabolite-conjugated and intense blue fluorescence-emitting smart polymeric nanoparticles is reported for the efficient delivery of anticancer drugs and self-monitoring their effect in drug-resistant metastatic breast cancer cells.

scholarly journals A Novel Approach against Salmonella: A Review of Polymeric Nanoparticle Vaccines for Broilers and Layers

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1041
Author(s):  
Keila Y. Acevedo-Villanueva ◽  
Gabriel O. Akerele ◽  
Walid Ghazi Al Hakeem ◽  
Sankar Renu ◽  
Revathi Shanmugasundaram ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Poultry Production ◽  
Potential Health ◽  
Mucosal Vaccination ◽  
Polymeric Nanoparticle ◽  
Effective Interventions ◽  
Novel Approach ◽  
Production And Consumption ◽  
Inactivated Vaccines ◽  
Global Population

This work discusses the present-day limitations of current commercial Salmonella vaccines for broilers and layers and explores a novel approach towards poultry vaccination using biodegradable nanoparticle vaccines against Salmonella. With the increasing global population and poultry production and consumption, Salmonella is a potential health risk for humans. The oral administration of killed or inactivated vaccines would provide a better alternative to the currently commercially available Salmonella vaccines for poultry. However, there are currently no commercial oral killed-vaccines against Salmonella for use in broilers or layers. There is a need for novel and effective interventions in the poultry industry. Polymeric nanoparticles could give way to an effective mass-administered mucosal vaccination method for Salmonella. The scope of this work is limited to polymeric nanoparticles against Salmonella for use in broilers and layers. This review is based on the information available at the time of the investigation.

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