Click Chemistry Functionalized Polymeric Nanoparticles Target Corneal Epithelial Cells through RGD-Cell Surface Receptors

2009 ◽  
Vol 20 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Jiao Lu ◽  
Meng Shi ◽  
Molly S. Shoichet
Keyword(s):  
Epithelial Cells ◽  
Cell Surface ◽  
Click Chemistry ◽  
Polymeric Nanoparticles ◽  
Cell Surface Receptors ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Surface Receptors

Corneal epithelial-specific cell surface antigen recognized by a monoclonal antibody

Development ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 163-172
Author(s):  
Marian G. Langer ◽  
C. V. Sundarraj ◽  
Nirmala Sundarraj
Keyword(s):  
Epithelial Cells ◽  
Cell Surface ◽  
Primary Cultures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Cell ◽  
Immunoperoxidase Technique ◽  
Igg Antibody ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells

Monoclonal antibodies, specific against cell surface differentiation antigens of human corneal epithelial cells, were developed using epithelial cells resected from human corneas as the immunogens. One of these antibodies reacted specifically with corneal epithelial cells and not with epithelial cells of other tissues when tested by an indirect immunoperoxidase technique. Nonidet P-40 extracts of different subcellular fractions of human corneal epithelial cells were tested for their reactivity against this antibody using an enzyme-linked immunosorbent assay. The results indicated that the antigen recognized by this antibody is associated with the plasma membrane. This was further verified by immuno-electron-microscopic analysis using ferritin-conjugated anti-mouse IgG antibody. This antigen was not detectable in the corneal epithelial cells in primary cultures nor in the epithelial cells from early stages of developing cornea (12 to 18 weeks in utero) but was present in the epithelial cells in the corneas of an 8-month-old infant. Therefore, this surface-associated antigen identified in the present study is a developmentally regulated marker of human corneal epithelium.

scholarly journals Cell Surface O-Glycans Limit Staphylococcus aureus Adherence to Corneal Epithelial Cells

2008 ◽  
Vol 76 (11) ◽  
pp. 5215-5220 ◽  
Author(s):  
Jessica Ricciuto ◽  
Susan R. Heimer ◽  
Michael S. Gilmore ◽  
Pablo Argüeso
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Cell Surface ◽  
Bacterial Adhesion ◽  
Surface Protein ◽  
Apical Surface ◽  
Accessory Gene ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Contact Lens Wear

ABSTRACT The mucin-rich environment of the intact corneal epithelium is thought to contribute to the prevention of Staphylococcus aureus infection. This study examined whether O-glycans, which constitute the majority of the mucin mass of epithelial cell glycocalyces, prevented bacterial adhesion and growth. Abrogation of mucin O glycosylation using the chemical primer benzyl-α-GalNAc resulted in increased adherence of parental strain RN6390 to apical human corneal-limbal epithelial (HCLE) cells and to biotinylated cell surface protein in static and liquid phase adhesion assays, consistent with a role of mucin O-glycans in preventing bacterial adhesion. Comparable results were found with ALC135, an isogenic mutant strain defective in the accessory gene regulators agr and sar, indicating that the agr- and/or sar-regulated virulence factors did not play a major role in mediating adhesion to the corneal cell surface after mucin O-glycan truncation. In exoglycosidase digestion studies, treatment with sialidase from Arthrobacter ureafaciens—which hydrolyzed mucin-associated O-acetyl sialic acid—but not from Clostridium perfringens resulted in an increase in RN6390 and ALC135 adhesion. Abrogation of mucin O glycosylation in HCLE cell cultures did not affect bacterial growth. Overall, these data indicate that mucin O-glycans contribute to the prevention of bacterial adherence to the apical surface of corneal epithelial cells and suggest that alteration of cell surface glycosylation from disease or trauma, including that stemming from contact lens wear, could contribute to a higher risk of infection.

scholarly journals Cell Surface Receptors: Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow-Based Microfluidic Device (Small 8/2015)

Small ◽  
2015 ◽  
Vol 11 (8) ◽  
pp. 1012-1012
Author(s):  
Ramesh Ramji ◽  
Cheong Fook Cheong ◽  
Hiroaki Hirata ◽  
Abdur Rub Abdur Rahman ◽  
Chwee Teck Lim
Keyword(s):  
Cell Surface ◽  
Microfluidic Device ◽  
Live Cell ◽  
Cell Surface Receptors ◽  
Cell Receptors ◽  
Surface Receptors ◽  
Rapid Quantification
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