The differential expression of mRNAs and long noncoding RNAs between ectopic and eutopic endometria provides new insights into adenomyosis

2016 ◽  
Vol 12 (2) ◽  
pp. 362-370 ◽  
Author(s):  
Cheng Zhou ◽  
Ting Zhang ◽  
Fei Liu ◽  
Ji Zhou ◽  
Xiaobei Ni ◽  
...  
Keyword(s):  
Differential Expression ◽  
Expression Patterns ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Lncrna Expression

This work constitutes the first known report on lncRNA expression patterns of ectopic and eutopic endometrial tissues in human adenomyosis.

TE composition of human long noncoding RNAs and their expression patterns in human tissues

2014 ◽  
Vol 37 (1) ◽  
pp. 87-95
Author(s):  
Donghee Kang ◽  
Yun-Ji Kim ◽  
Kwonho Hong ◽  
Kyudong Han
Keyword(s):  
Expression Patterns ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Human Tissues

scholarly journals Genome-wide expression profiling in colorectal cancer focusing on lncRNAs in the adenoma-carcinoma transition

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Alexandra Kalmár ◽  
Zsófia Brigitta Nagy ◽  
Orsolya Galamb ◽  
István Csabai ◽  
András Bodor ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Differential Expression ◽  
Colorectal Adenoma ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Aberrant Expression ◽  
Array Data ◽  
Lncrna Expression ◽  
Qrt Pcr ◽  
Mrna Targets

Abstract Background Long non-coding RNAs (lncRNAs) play a fundamental role in colorectal cancer (CRC) development, however, lncRNA expression profiles in CRC and its precancerous stages remain to be explored. We aimed to study whole genomic lncRNA expression patterns in colorectal adenoma–carcinoma transition and to analyze the underlying functional interactions of aberrantly expressed lncRNAs. Methods LncRNA expression levels of colonic biopsy samples (20 CRCs, 20 adenomas (Ad), 20 healthy controls (N)) were analyzed with Human Transcriptome Array (HTA) 2.0. Expression of a subset of candidates was verified by qRT-PCR and in situ hybridization (ISH) analyses. Furthermore, in silico validation was performed on an independent HTA 2.0, on HGU133Plus 2.0 array data and on the TCGA COAD dataset. MiRNA targets of lncRNAs were predicted with miRCODE and lncBase v2 algorithms and miRNA expression was analyzed on miRNA3.0 Array data. MiRNA-mRNA target prediction was performed using miRWALK and c-Met protein levels were analyzed by immunohistochemistry. Comprehensive lncRNA-mRNA-miRNA co-expression pattern analysis was also performed. Results Based on our HTA results, a subset of literature-based CRC-associated lncRNAs showed remarkable expression changes already in precancerous colonic lesions. In both Ad vs. normal and CRC vs. normal comparisons 16 lncRNAs, including downregulated LINC02023, MEG8, AC092834.1, and upregulated CCAT1, CASC19 were identified showing differential expression during early carcinogenesis that persisted until CRC formation (FDR-adjusted p < 0.05). The intersection of CRC vs. N and CRC vs. Ad comparisons defines lncRNAs characteristic of malignancy in colonic tumors, where significant downregulation of LINC01752 and overexpression of UCA1 and PCAT1 were found. Two candidates with the greatest increase in expression in the adenoma-carcinoma transition were further confirmed by qRT-PCR (UCA1, CCAT1) and by ISH (UCA1). In line with aberrant expression of certain lncRNAs in tumors, the expression of miRNA and mRNA targets showed systematic alterations. For example, UCA1 upregulation in CRC samples occurred in parallel with hsa-miR-1 downregulation, accompanied by c-Met target mRNA overexpression (p < 0.05). Conclusion The defined lncRNA sets may have a regulatory role in the colorectal adenoma-carcinoma transition. A subset of CRC-associated lncRNAs showed significantly differential expression in precancerous samples, raising the possibility of developing adenoma-specific markers for early detection of colonic lesions.

A preliminary study on the differential expression of long noncoding RNAs and messenger RNAs in obese and control mice

2019 ◽  
Vol 121 (2) ◽  
pp. 1126-1143 ◽  
Author(s):  
Ping Li ◽  
Xiaoyu Chen ◽  
Xuelian Chang ◽  
Tiantian Tang ◽  
Kemin Qi
Keyword(s):  
Differential Expression ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Messenger Rnas ◽  
Preliminary Study ◽  
And Control

scholarly journals Spatiotemporal expression and transcriptional perturbations by long noncoding RNAs in the mouse brain

2015 ◽  
Vol 112 (22) ◽  
pp. 6855-6862 ◽  
Author(s):  
Loyal A. Goff ◽  
Abigail F. Groff ◽  
Martin Sauvageau ◽  
Zachary Trayes-Gibson ◽  
Diana B. Sanchez-Gomez ◽  
...  
Keyword(s):  
Neural Development ◽  
Expression Patterns ◽  
Noncoding Rnas ◽  
Brain Regions ◽  
Long Noncoding Rnas ◽  
Adult Brain ◽  
Mammalian Brain ◽  
Endogenous Expression ◽  
Spatiotemporal Expression

Long noncoding RNAs (lncRNAs) have been implicated in numerous cellular processes including brain development. However, the in vivo expression dynamics and molecular pathways regulated by these loci are not well understood. Here, we leveraged a cohort of 13 lncRNA-null mutant mouse models to investigate the spatiotemporal expression of lncRNAs in the developing and adult brain and the transcriptome alterations resulting from the loss of these lncRNA loci. We show that several lncRNAs are differentially expressed both in time and space, with some presenting highly restricted expression in only selected brain regions. We further demonstrate altered regulation of genes for a large variety of cellular pathways and processes upon deletion of the lncRNA loci. Finally, we found that 4 of the 13 lncRNAs significantly affect the expression of several neighboring protein-coding genes in a cis-like manner. By providing insight into the endogenous expression patterns and the transcriptional perturbations caused by deletion of the lncRNA locus in the developing and postnatal mammalian brain, these data provide a resource to facilitate future examination of the specific functional relevance of these genes in neural development, brain function, and disease.

scholarly journals Differential Expression of Long Noncoding RNAs in Human Cumulus Cells Related to Embryo Developmental Potential

2014 ◽  
Vol 22 (6) ◽  
pp. 672-678 ◽  
Author(s):  
Xiao-Feng Xu ◽  
Juan Li ◽  
Yun-Xia Cao ◽  
Da-Wei Chen ◽  
Zhi-Guo Zhang ◽  
...  
Keyword(s):  
Differential Expression ◽  
Noncoding Rnas ◽  
Cumulus Cells ◽  
Long Noncoding Rnas ◽  
Developmental Potential
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