A preliminary study on the differential expression of long noncoding RNAs and messenger RNAs in obese and control mice

2019 ◽  
Vol 121 (2) ◽  
pp. 1126-1143 ◽  
Author(s):  
Ping Li ◽  
Xiaoyu Chen ◽  
Xuelian Chang ◽  
Tiantian Tang ◽  
Kemin Qi
Keyword(s):  
Differential Expression ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Messenger Rnas ◽  
Preliminary Study ◽  
And Control

Crosstalk between prognostic long noncoding RNAs and messenger RNAs as transcriptional hallmarks in gastric cancer

Epigenomics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 433-443 ◽  
Author(s):  
Jian Zhang ◽  
Yujie Yuan ◽  
Zhewei Wei ◽  
Jianwei Ren ◽  
Xun Hou ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Messenger Rnas

scholarly journals Differential Expression of Long Noncoding RNAs in Human Cumulus Cells Related to Embryo Developmental Potential

2014 ◽  
Vol 22 (6) ◽  
pp. 672-678 ◽  
Author(s):  
Xiao-Feng Xu ◽  
Juan Li ◽  
Yun-Xia Cao ◽  
Da-Wei Chen ◽  
Zhi-Guo Zhang ◽  
...  
Keyword(s):  
Differential Expression ◽  
Noncoding Rnas ◽  
Cumulus Cells ◽  
Long Noncoding Rnas ◽  
Developmental Potential

scholarly journals Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma

2019 ◽  
Vol 2019 ◽  
pp. 1-16
Author(s):  
Fei Liu ◽  
Jiazhang Wei ◽  
Yanrong Hao ◽  
Fengzhu Tang ◽  
Wei Jiao ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Fatty Acid Synthase ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Messenger Rnas ◽  
Regulatory Pathways ◽  
Different Levels

Our previous studies showed that ZBTB7A played an important role in promoting nasopharyngeal carcinoma (NPC) progression. However, molecular mechanisms of different levels of ZBTB7A are still unclear. It is necessary to search molecular markers which are closely connected with ZBTB7A. We selected NPC sublines CNE2 with stably transfecting empty plasmid (negative control, NC) and short hair RNA (shRNA) plasmid targeting ZBTB7A as research objectives. Microarray was used to screen differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) via shRNA-CNE2 versus NC-CNE2. Quantitative PCR (qPCR) was used to validate lncRNAs and mRNAs from the sublines, chronic rhinitis, and NPC tissues. Bioinformatics was used to analyze regulatory pathways which were connected with ZBTB7A. The 1501 lncRNAs (long noncoding RNAs) and 1275 differentially expressed mRNAs were upregulated or downregulated over 2-fold. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the upregulated or downregulated carbohydrate and lipid metabolisms probably involved in carcinogenicity of shRNA-CNE2 (P-value cut-off was 0.05). In order to find the molecular mechanisms of ZBTB7A, we validated 12 differentially expressed lncRNAs and their nearby mRNAs by qPCR. Most of the differentially expressed mRNAs are closely connected with carbohydrate and lipid metabolisms in multiply cancers. Furthermore, part of them were validated in NPC and rhinitis tissues by qPCR. As a result, NR_047538, ENST00000442852, and fatty acid synthase (FASN) were closely associated with NPC. ZBTB7A had a positive association with NR_047538 and negative associations with ENST00000442852 and FASN. The results probably provide novel candidate biomarkers for NPC progression with different levels of ZBTB7A.

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