Continuous inertial microparticle and blood cell separation in straight channels with local microstructures

Lab on a Chip ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. 532-542 ◽  
Author(s):  
Zhenlong Wu ◽  
Yu Chen ◽  
Moran Wang ◽  
Aram J. Chung
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Cell Separation ◽  
High Efficiency

We present an inertial platform for high-throughput, high-efficiency and sheathless particle and blood cell separation in straight channels with microstructures.

Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation

Lab on a Chip ◽  
2011 ◽  
Vol 11 (11) ◽  
pp. 1870 ◽  
Author(s):  
Ali Asgar S. Bhagat ◽  
Han Wei Hou ◽  
Leon D. Li ◽  
Chwee Teck Lim ◽  
Jongyoon Han
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Cell Separation ◽  
Inertial Microfluidics

Development and study of a method for cell separation during white blood cell segmentation on images of bone marrow preparations in information and measurement systems for diagnostics of acute leukemia

2020 ◽  
pp. 68-72
Author(s):  
V.G. Nikitaev ◽  
A.N. Pronichev ◽  
V.V. Dmitrieva ◽  
E.V. Polyakov ◽  
A.D. Samsonova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Acute Leukemia ◽  
White Blood Cell ◽  
Cell Separation ◽  
Large Scale ◽  
Bone Marrow Cells ◽  
Automated Analysis ◽  
Cell Segmentation ◽  
Measurement Systems

The issues of using of information and measurement systems based on processing of digital images of microscopic preparations for solving large-scale tasks of automating the diagnosis of acute leukemia are considered. The high density of leukocyte cells in the preparation (hypercellularity) is a feature of microscopic images of bone marrow preparations. It causes the proximity of cells to eachother and their contact with the formation of conglomerates. Measuring of the characteristics of bone marrow cells in such conditions leads to unacceptable errors (more than 50%). The work is devoted to segmentation of contiguous cells in images of bone marrow preparations. A method of cell separation during white blood cell segmentation on images of bone marrow preparations under conditions of hypercellularity of the preparation has been developed. The peculiarity of the proposed method is the use of an approach to segmentation of cell images based on the watershed method with markers. Key stages of the method: the formation of initial markers and builds the lines of watershed, a threshold binarization, shading inside the outline. The parameters of the separation of contiguous cells are determined. The experiment confirmed the effectiveness of the proposed method. The relative segmentation error was 5 %. The use of the proposed method in information and measurement systems of computer microscopy for automated analysis of bone marrow preparations will help to improve the accuracy of diagnosis of acute leukemia.

Blood Cell Separation Device Using Serially Connected Membrane Filters for Adapting to Blood Flow Properties

2009 ◽  
Vol 129 (11) ◽  
pp. 380-386 ◽  
Author(s):  
Taizo Kobayashi ◽  
Daiki Kato ◽  
Hiroyuki Koga ◽  
Kenichi Morimoto ◽  
Makoto Fukuda ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Cell ◽  
Cell Separation ◽  
Flow Properties ◽  
Separation Device ◽  
Membrane Filters

Flow-Field-Assisted Dielectrophoretic Microchips for High-Efficiency Sheathless Particle/Cell Separation with Dual Mode

2021 ◽  
Author(s):  
Shitao Shen ◽  
Zichuan Yi ◽  
Xing Li ◽  
Shuting Xie ◽  
Mingliang Jin ◽  
...  
Keyword(s):  
Flow Field ◽  
Cell Separation ◽  
High Efficiency ◽  
Dual Mode

scholarly journals EM by EM: High-Efficiency Epitope Mapping using High-Throughput Electron Microscopy

2016 ◽  
Vol 22 (S3) ◽  
pp. 1080-1081
Author(s):  
Alberto Estevez ◽  
Colin Garvey ◽  
Claudio Ciferri
Keyword(s):  
Electron Microscopy ◽  
High Throughput ◽  
Epitope Mapping ◽  
High Efficiency

Clog-free cell filtration using resettable cell traps

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2657-2665 ◽  
Author(s):  
William Beattie ◽  
Xi Qin ◽  
Lin Wang ◽  
Hongshen Ma
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Free Cell ◽  
Separation Mechanism ◽  
Cell Filtration ◽  
Capture And Release

A microfluidic cell separation mechanism created using constrictions with adjustable size that can selectively capture and release cells, thereby enabling high throughput size and deformability based cell separation without clogging.

Open-Tubular Capillary Cell Affinity Chromatography:  Single and Tandem Blood Cell Separation

2008 ◽  
Vol 80 (6) ◽  
pp. 2118-2124 ◽  
Author(s):  
Kelong Wang ◽  
Molly K. Marshall ◽  
Griselda Garza ◽  
Dimitri Pappas
Keyword(s):  
Blood Cell ◽  
Affinity Chromatography ◽  
Cell Separation ◽  
Cell Affinity

The new concept of flux enhancement during cell separation with MF/UF processes

2001 ◽  
Vol 1 (5-6) ◽  
pp. 381-386
Author(s):  
A. Kołtuniewicz
Keyword(s):  
Cell Separation ◽  
High Efficiency ◽  
Membrane Surface ◽  
Cross Flow ◽  
Dry Mass ◽  
High Energy ◽  
Process Conditions ◽  
Permeate Flux ◽  
Flux Enhancement ◽  
Flow Systems

The microfiltration and ultrafiltration processes are considered as matured membrane processes that are well established in industrial practice. Nevertheless, the main obstacles of their further development in the new competitive implementations are the economical problems. The key economic factors are permeate flux and energy consumption. However, although the cross-flow systems enable us to attain higher flux, it is usually very expensive. The high energy is consumed to maintain circulation velocity of the retentate that is sufficient for sweeping out the retained component from the membrane surface. Moreover in the case of cells separation the high intensity of the fouling and low cake permeability makes it necessary to apply additional efforts, such as backflushing, backpulsing, promoters of turbulence, vibrations, ultrasounds and many other. Therefore, dead-end systems are still quite competitive with cross-flow, especially for diluted (less than 0.5% of dry mass) suspensions or solutions. Cell separation with membranes is one of the most vivid problems for modern biotechnology, wastewater and water treatment. Membranes offer mild process conditions and high selectivity of separation. This enables us to solve a variety of problems such as cell culturing, fractionation, concentration, purification and sterilisation. The selected cells may be precisely separated from other components of broth and subsequently directed into the reaction space again in good conditions to ensure a quasi-continuous mode of operation. Moreover, membranes enable us to attain high efficiency of the bioconversion by removal of all product and inhibitors directly from the bioreactor. This is the reason for the huge interest in cell separation with membranes. The idea of the paper was to present the new concept of flux enhancement for cell separation on membranes. This concept lies in taking advantage of the specific rheological nature of biopolymers, which are the main foulants. The biopolymers retained on the membrane surface (i.e. on the top layer) can be applied as a lubricant for the cells that can settle on such a ‘movable layer’. As is shown, further in the paper, the thickness of the moving layer is lower and the flux is greater. The common movement of the cells and gel layer is very convenient from the cells integrity point of view. However the hydrodynamic conditions always play an important role in cross-flow systems; the resistance of ultrafiltration membranes may be reduced much more when compared with more open microfiltration membranes.

Systematic Use Of Heparin In The Continuous Flow Centrifuge (CFC) For Blood Cell Separation

1981 ◽  
Author(s):  
M R Morales ◽  
J Pizzuto ◽  
Ma Reyna ◽  
G Castro
Keyword(s):  
Blood Cell ◽  
Continuous Flow ◽  
Cell Separation ◽  
Reliable Method ◽  
Dosage Level ◽  
Thrombin Time ◽  
Coagulation Disorders ◽  
Time Dilution ◽  
Continuous Flow Centrifuge ◽  
Simultaneous Assessment

To date the use of heparin in the CFC has not been adequately controlled, thus exposing donors and patients to coagulation disorders. For this reason, we decided to evaluate the use of heparin by continuous infusion in dosages that would be modified by a simultaneous assessment of its anticoagulant effect, as shown by the thrombin time dilution test (TTDT).The study was performed during 46 leukopher- esis and 27 plasmapheresis. It was ascertained that heparin is an efficient anticoagulant in the CFC, using the TTDT as an effective and reliable method for its control. The initial dose in leukopheresis is one unit per milliliter of blood during the first hour, then half the dose during the next hour, and then a quarter of the dose until the procedure is completed. A TTDT performed every hour will indicate whether the amount of heparin used should be modified. For plasmapheresis, it is neccesary to establish a specific dose in each instance, using the TTDT as described. In most of the subjects, the anticoagulant level was exactly right. There was no case of bleeding or extracorporeal coagulation of the blood.On the basis of these findings, we recommend the use of heparin in the CFC, applying the results of the TTDT as a guide for its dosage level.

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