Open-Tubular Capillary Cell Affinity Chromatography:  Single and Tandem Blood Cell Separation

2008 ◽  
Vol 80 (6) ◽  
pp. 2118-2124 ◽  
Author(s):  
Kelong Wang ◽  
Molly K. Marshall ◽  
Griselda Garza ◽  
Dimitri Pappas
Keyword(s):  
Blood Cell ◽  
Affinity Chromatography ◽  
Cell Separation ◽  
Cell Affinity

Development and study of a method for cell separation during white blood cell segmentation on images of bone marrow preparations in information and measurement systems for diagnostics of acute leukemia

2020 ◽  
pp. 68-72
Author(s):  
V.G. Nikitaev ◽  
A.N. Pronichev ◽  
V.V. Dmitrieva ◽  
E.V. Polyakov ◽  
A.D. Samsonova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Acute Leukemia ◽  
White Blood Cell ◽  
Cell Separation ◽  
Large Scale ◽  
Bone Marrow Cells ◽  
Automated Analysis ◽  
Cell Segmentation ◽  
Measurement Systems

The issues of using of information and measurement systems based on processing of digital images of microscopic preparations for solving large-scale tasks of automating the diagnosis of acute leukemia are considered. The high density of leukocyte cells in the preparation (hypercellularity) is a feature of microscopic images of bone marrow preparations. It causes the proximity of cells to eachother and their contact with the formation of conglomerates. Measuring of the characteristics of bone marrow cells in such conditions leads to unacceptable errors (more than 50%). The work is devoted to segmentation of contiguous cells in images of bone marrow preparations. A method of cell separation during white blood cell segmentation on images of bone marrow preparations under conditions of hypercellularity of the preparation has been developed. The peculiarity of the proposed method is the use of an approach to segmentation of cell images based on the watershed method with markers. Key stages of the method: the formation of initial markers and builds the lines of watershed, a threshold binarization, shading inside the outline. The parameters of the separation of contiguous cells are determined. The experiment confirmed the effectiveness of the proposed method. The relative segmentation error was 5 %. The use of the proposed method in information and measurement systems of computer microscopy for automated analysis of bone marrow preparations will help to improve the accuracy of diagnosis of acute leukemia.

Blood Cell Separation Device Using Serially Connected Membrane Filters for Adapting to Blood Flow Properties

2009 ◽  
Vol 129 (11) ◽  
pp. 380-386 ◽  
Author(s):  
Taizo Kobayashi ◽  
Daiki Kato ◽  
Hiroyuki Koga ◽  
Kenichi Morimoto ◽  
Makoto Fukuda ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Cell ◽  
Cell Separation ◽  
Flow Properties ◽  
Separation Device ◽  
Membrane Filters

Systematic Use Of Heparin In The Continuous Flow Centrifuge (CFC) For Blood Cell Separation

1981 ◽  
Author(s):  
M R Morales ◽  
J Pizzuto ◽  
Ma Reyna ◽  
G Castro
Keyword(s):  
Blood Cell ◽  
Continuous Flow ◽  
Cell Separation ◽  
Reliable Method ◽  
Dosage Level ◽  
Thrombin Time ◽  
Coagulation Disorders ◽  
Time Dilution ◽  
Continuous Flow Centrifuge ◽  
Simultaneous Assessment

To date the use of heparin in the CFC has not been adequately controlled, thus exposing donors and patients to coagulation disorders. For this reason, we decided to evaluate the use of heparin by continuous infusion in dosages that would be modified by a simultaneous assessment of its anticoagulant effect, as shown by the thrombin time dilution test (TTDT).The study was performed during 46 leukopher- esis and 27 plasmapheresis. It was ascertained that heparin is an efficient anticoagulant in the CFC, using the TTDT as an effective and reliable method for its control. The initial dose in leukopheresis is one unit per milliliter of blood during the first hour, then half the dose during the next hour, and then a quarter of the dose until the procedure is completed. A TTDT performed every hour will indicate whether the amount of heparin used should be modified. For plasmapheresis, it is neccesary to establish a specific dose in each instance, using the TTDT as described. In most of the subjects, the anticoagulant level was exactly right. There was no case of bleeding or extracorporeal coagulation of the blood.On the basis of these findings, we recommend the use of heparin in the CFC, applying the results of the TTDT as a guide for its dosage level.

Barrier Blood Cell Separation Methods Used With Healthy and HIV-Positive Individuals

1992 ◽  
Vol 23 (10) ◽  
pp. 658-662
Author(s):  
Peter A. Takes ◽  
Linda Rinker ◽  
Robert D. Krug
Keyword(s):  
Blood Cell ◽  
Cell Separation ◽  
Hiv Positive

scholarly journals Magnetic Microfluidic Linear Halbach Array Configuration for Cell Separation

2018 ◽  
Vol 153 ◽  
pp. 06008
Author(s):  
Omer Saeed ◽  
Iucienne Duru ◽  
Deng Yulin
Keyword(s):  
Blood Cell ◽  
Critical Point ◽  
Tumor Cells ◽  
Blood Cells ◽  
Recovery Rate ◽  
Cell Separation ◽  
Comsol Multiphysics ◽  
Array Configuration ◽  
Medical Tests ◽  
Halbach Array

The different type of blood entities like red blood cells (RBCs), white blood cell (WBCs) and platelets counts is a critical point in routine medical tests. Circulating tumor cells (CTCs) counting and analysing in the blood is indicative of the stage and origin of cancer in particular time. Here we are describing a proposed microfluidic device, used a magnetic means for CTCs separation from a blood or buffer sample. The device characterizations were simulated using COMSOL MULTIPHYSICS software as well as practically validated. Using a known spiked number of CTCs in the sample, the purity and recovery rate were calculated to be 93% and respectively.

Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation

Lab on a Chip ◽  
2011 ◽  
Vol 11 (11) ◽  
pp. 1870 ◽  
Author(s):  
Ali Asgar S. Bhagat ◽  
Han Wei Hou ◽  
Leon D. Li ◽  
Chwee Teck Lim ◽  
Jongyoon Han
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Cell Separation ◽  
Inertial Microfluidics
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