Single chip SPR and fluorescent ELISA assay of prostate specific antigen

Lab on a Chip ◽  
2015 ◽  
Vol 15 (23) ◽  
pp. 4433-4440 ◽  
Author(s):  
J. Breault-Turcot ◽  
H.-P. Poirier-Richard ◽  
M. Couture ◽  
D. Pelechacz ◽  
J.-F. Masson
Keyword(s):  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Fluorescence Detection ◽  
Plasmon Resonance ◽  
Specific Antigen ◽  
Fluorescence Microscope ◽  
Elisa Assay ◽  
Single Chip ◽  
Channel System

A multi-channel system combining fluidics and micropatterned plasmonic materials with wavelength interrogation surface plasmon resonance (SPR) and fluorescence detection was integrated from the combination of a small and motorized fluorescence microscope mounted on a portable 4-channel SPR instrument.

Signal amplification on planar and gel-type sensor surfaces in surface plasmon resonance-based detection of prostate-specific antigen

2004 ◽  
Vol 333 (1) ◽  
pp. 165-173 ◽  
Author(s):  
Geert A.J. Besselink ◽  
Rob P.H. Kooyman ◽  
Peter J.H.J. van Os ◽  
Gerard H.M. Engbers ◽  
Richard B.M. Schasfoort
Keyword(s):  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Specific Antigen

Initial Study of Thin-Film Derived Gold Nanoparticles (Au NPs) Surface Plasmon Resonance (SPR) Based Prostate-Specific Antigen (PSA) Detection

2020 ◽  
Vol 18 (11) ◽  
pp. 781-784
Author(s):  
M. Dhanunjaya ◽  
T. Sonklin ◽  
Pimchanok Leuasoongnoen ◽  
Sanong Suksaweang ◽  
Pattanaphong Janphuang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Specific Antigen ◽  
Initial Study ◽  
Peak Position ◽  
Localized Surface Plasmon ◽  
Au Nps

The gold nanoparticles (Au NPs) localized surface plasmon resonance (LSPR) based detection of prostate-specific antigen (PSA) biomarker was carried out in this work. Au NPs on Si and quartz substrates were synthesized by DC magnetron sputtering method followed by conventional annealing protocol. The functionalization of antibody and PSA with Au NPs was evaluated using a simple drop-cost method. The SPR peak intensity was found to be proportional to the PSA concentration. The redshift in SPR peak position might be owing to the change in refractive index around the Au NPs with the conjugation of antibody and further additional binding of specific PSA at different concentrations.

scholarly journals A Wedge-Shaped Au Thin Film: Integrating Multiple Surface Plasmon Resonance Sensors in a Single Chip and Enhancing the Figure of Merit

2021 ◽  
Vol 3 ◽  
Author(s):  
Hiromasa Shimizu ◽  
Takahiro Ogura ◽  
Takumi Maeda ◽  
Shogo Suzuki
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Figure Of Merit ◽  
Refractive Index Change ◽  
Electron Beam Evaporation ◽  
Attenuated Total Reflection ◽  
Single Chip ◽  
Au Thin Film

We show here the design, fabrication, and characterization of a wedge-shaped Au thin film with an enhanced figure of merit (FOM). This is achieved by using a reflectivity change in an attenuated total reflection (ATR) setup by slightly modulating the wavenumber of the surface plasmon polariton by means of the varying thickness of the Au thin film. The wedge-shaped Au thin film is equivalent to multiple surface plasmon resonance (SPR) transducers integrated in a single chip and was fabricated by an electron-beam evaporation process with the position of the shutter controlled during the deposition. The FOM, defined as the difference between the maximum and minimum values of the normalized reflectivity change (ΔR/R) divided by the corresponding difference of the incident angles, was 8.0-times larger than that based on the reflectivity R. Also, we demonstrated that the wedge-shaped Au thin film was able to detect ethanol gas at a concentration of 0.2%, corresponding to a refractive index change of 2 × 10−5, without any surface functionalization. Since the sensing signal can be obtained with a single image from the wedge-shaped Au thin film without precise thickness control of the metal thickness, no other materials or modulation equipment is necessary, and the sensing chip can be employed in simple and highly sensitive systems.

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