An S-Layer Heavy Chain Camel Antibody Fusion Protein for Generation of a Nanopatterned Sensing Layer To Detect the Prostate-Specific Antigen by Surface Plasmon Resonance Technology

2004 ◽  
Vol 15 (3) ◽  
pp. 664-671 ◽  
Author(s):  
Magdalena Pleschberger ◽  
Dirk Saerens ◽  
Stefan Weigert ◽  
Uwe B. Sleytr ◽  
Serge Muyldermans ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Fusion Protein ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Heavy Chain ◽  
Plasmon Resonance ◽  
Specific Antigen ◽  
Antibody Fusion Protein

Single chip SPR and fluorescent ELISA assay of prostate specific antigen

Lab on a Chip ◽  
2015 ◽  
Vol 15 (23) ◽  
pp. 4433-4440 ◽  
Author(s):  
J. Breault-Turcot ◽  
H.-P. Poirier-Richard ◽  
M. Couture ◽  
D. Pelechacz ◽  
J.-F. Masson
Keyword(s):  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Fluorescence Detection ◽  
Plasmon Resonance ◽  
Specific Antigen ◽  
Fluorescence Microscope ◽  
Elisa Assay ◽  
Single Chip ◽  
Channel System

A multi-channel system combining fluidics and micropatterned plasmonic materials with wavelength interrogation surface plasmon resonance (SPR) and fluorescence detection was integrated from the combination of a small and motorized fluorescence microscope mounted on a portable 4-channel SPR instrument.

Signal amplification on planar and gel-type sensor surfaces in surface plasmon resonance-based detection of prostate-specific antigen

2004 ◽  
Vol 333 (1) ◽  
pp. 165-173 ◽  
Author(s):  
Geert A.J. Besselink ◽  
Rob P.H. Kooyman ◽  
Peter J.H.J. van Os ◽  
Gerard H.M. Engbers ◽  
Richard B.M. Schasfoort
Keyword(s):  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Specific Antigen

Initial Study of Thin-Film Derived Gold Nanoparticles (Au NPs) Surface Plasmon Resonance (SPR) Based Prostate-Specific Antigen (PSA) Detection

2020 ◽  
Vol 18 (11) ◽  
pp. 781-784
Author(s):  
M. Dhanunjaya ◽  
T. Sonklin ◽  
Pimchanok Leuasoongnoen ◽  
Sanong Suksaweang ◽  
Pattanaphong Janphuang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Prostate Specific Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Specific Antigen ◽  
Initial Study ◽  
Peak Position ◽  
Localized Surface Plasmon ◽  
Au Nps

The gold nanoparticles (Au NPs) localized surface plasmon resonance (LSPR) based detection of prostate-specific antigen (PSA) biomarker was carried out in this work. Au NPs on Si and quartz substrates were synthesized by DC magnetron sputtering method followed by conventional annealing protocol. The functionalization of antibody and PSA with Au NPs was evaluated using a simple drop-cost method. The SPR peak intensity was found to be proportional to the PSA concentration. The redshift in SPR peak position might be owing to the change in refractive index around the Au NPs with the conjugation of antibody and further additional binding of specific PSA at different concentrations.

scholarly journals Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1003 ◽  
Author(s):  
Jin-Ha Choi ◽  
Jin-Ho Lee ◽  
Joohyung Son ◽  
Jeong-Woo Choi
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Noble Metals ◽  
Specific Antigen ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Biomarkers ◽  
Detection Probe ◽  
Inducing Factors

For the early diagnosis of several diseases, various biomarkers have been discovered and utilized through the measurement of concentrations in body fluids such as blood, urine, and saliva. The most representative analytical method for biomarker detection is an immunosensor, which exploits the specific antigen-antibody immunoreaction. Among diverse analytical methods, surface plasmon resonance (SPR)-based immunosensors are emerging as a potential detection platform due to high sensitivity, selectivity, and intuitive features. Particularly, SPR-based immunosensors could detect biomarkers without labeling of a specific detection probe, as typical immunosensors such as enzyme-linked immunosorbent assay (ELISA) use enzymes like horseradish peroxidase (HRP). In this review, SPR-based immunosensors utilizing noble metals such as Au and Ag as SPR-inducing factors for the measurement of different types of protein biomarkers, including viruses, microbes, and extracellular vesicles (EV), are briefly introduced.

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