Self-powered switch-controlled nucleic acid extraction system

Lab on a Chip ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 132-141 ◽  
Author(s):  
Kyungsup Han ◽  
Yong-Jin Yoon ◽  
Yong Shin ◽  
Mi Kyoung Park
Keyword(s):  
Nucleic Acid ◽  
Dna Extraction ◽  
Extraction System ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
System P ◽  
Self Powered ◽  
User Friendly

We have developed a Self-powered Switch-controlled Nucleic acid Extraction System (SSNES) to overcome the limitation of LOC technology in POC applications. The SSNES have a potential to be widely used as powerless, fully-disposable and user-friendly system for DNA extraction.

scholarly journals Comparison of Six Commercial DNA Extraction Kits for Recovery of Cytomegalovirus DNA from Spiked Human Specimens

2000 ◽  
Vol 38 (10) ◽  
pp. 3860-3863 ◽  
Author(s):  
Gary A. Fahle ◽  
Steven H. Fischer
Keyword(s):  
Nucleic Acid ◽  
Dna Extraction ◽  
Cerebral Spinal Fluid ◽  
Processing Time ◽  
Dna Isolation ◽  
Dna Purification ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Pcr Inhibitors ◽  
Positive Results

We evaluated six commercially available DNA extraction kits for their ability to recover DNA from various dilutions of cytomegalovirus (CMV) added to four different specimens: bronchoalveolar lavage, cerebral spinal fluid, plasma, and whole blood. The kits evaluated included the Puregene DNA isolation kit (PG), Generation Capture Column kit, MasterPure DNA purification kit, IsoQuick nucleic acid extraction kit, QIAamp blood kit, and NucliSens isolation kit (NS). All six kits evaluated effectively removed PCR inhibitors from each of the four specimen types and produced consistently positive results down to a spiked concentration of 200 PFU of whole CMV per ml. However, the NS and PG resulted in the most consistently positive results at the lowest concentrations of spiked CMV (4 and 0.4 PFU/ml) and, in this evaluation, offered the most sensitive methods for extracting CMV DNA from the four different spiked specimens. Processing time and cost were also evaluated.

Effects of diverse materials-based methods on DNA extraction for Clostridium difficle from stool samples

2019 ◽  
Vol 9 (5) ◽  
pp. 509-516 ◽  
Author(s):  
Ziqi Xiao ◽  
Gaojian Yang ◽  
Deng Yan ◽  
Song Li ◽  
Zhu Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Extraction ◽  
Magnetic Beads ◽  
Diagnostic Technique ◽  
Proteinase K ◽  
Molecular Diagnostic ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Spin Column ◽  
Stool Samples

Nosocomial infections, including Clostridium difficile infection (CDI), and their fatality rates have increased in the past few decades. Despite emerging molecular diagnostic technologies with rapid, accurate outcomes, nucleic acid extraction from stool samples remains the first limiting step before downstream applications. Commercial nucleic acid extraction kits greatly decrease labor and time requirements, and also provide nucleic acid preparations with higher quality and purity for enzyme digestion analysis or genotyping. The magnetic bead based technique is a novel method compared with the conventional spin-column method, and currently has widespread use in nucleic acid extraction. We evaluated five DNA extraction kits with magnetic beads using materials with various properties (particle size, concentration of magnetic beads, grinding beads) and reagents (proteinase K, lysozyme, isopropanol, and absolute ethanol) to determine the cost, hands-on time, number of essential operations, and quality and purity of the DNA preparations, compared with those obtained using the QIAamp Fast DNA Stool Mini Kit. The six DNA extraction kits yielded A260/280 ratios ranging from 0.85 to 1.9 (average 1.57), and concentrations from 3.70 to 108.09 ng/μL (average 34.64 ng/μL). All the DNA samples had acceptable downstream application effects, except for those obtained using the TIANGEN Magnetic Soil and Stool DNA Kit. However, gel electrophoresis analysis of the DNA samples resulted in a light strip on the gel, indicating that the proteinaceous contaminant may not have been removed completely. A rapid and accurate molecular diagnostic technique could allow for more suitable treatment and prognosis outcomes for inpatients, depending, in large part, on the quality and purity of DNA preparations, which are frequently neglected. Our study focused on the quality of commercial kits with a primary focus on the treatment of stool samples and molecular diagnostic applications.

scholarly journals Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens

2012 ◽  
Vol 84 (6) ◽  
pp. 906-911 ◽  
Author(s):  
C. Mengelle ◽  
J.-M. Mansuy ◽  
K. Sandres-Sauné ◽  
C. Barthe ◽  
J. Boineau ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Extraction System ◽  
Acid Extraction ◽  
Prospective Evaluation ◽  
Nucleic Acid Extraction ◽  
Respiratory Specimens

A fast nucleic acid extraction system for point-of-care and integration of digital PCR

The Analyst ◽  
2019 ◽  
Vol 144 (23) ◽  
pp. 7032-7040 ◽  
Author(s):  
Juxin Yin ◽  
Jiumei Hu ◽  
Jingjing Sun ◽  
Ben Wang ◽  
Ying Mu
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Digital Pcr ◽  
Extraction System ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

This work showcases a PTFE-based nucleic acid extraction system for point-of-care and integration of digital PCR.

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