A fast nucleic acid extraction system for point-of-care and integration of digital PCR

The Analyst ◽  
2019 ◽  
Vol 144 (23) ◽  
pp. 7032-7040 ◽  
Author(s):  
Juxin Yin ◽  
Jiumei Hu ◽  
Jingjing Sun ◽  
Ben Wang ◽  
Ying Mu
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Digital Pcr ◽  
Extraction System ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

This work showcases a PTFE-based nucleic acid extraction system for point-of-care and integration of digital PCR.

Equipment-free nucleic acid extraction and amplification on a simple paper disc for point-of-care diagnosis of rotavirus A

2018 ◽  
Vol 1018 ◽  
pp. 78-85 ◽  
Author(s):  
Xin Ye ◽  
Jin Xu ◽  
Lijuan Lu ◽  
Xinxin Li ◽  
Xueen Fang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Rotavirus A ◽  
Paper Disc

scholarly journals A point of care platform based on microfluidic chip for nucleic acid extraction in less than 1 minute

2019 ◽  
Vol 13 (3) ◽  
pp. 034102 ◽  
Author(s):  
Jianzhong Zhang ◽  
Xiaosong Su ◽  
Jiasu Xu ◽  
Jin Wang ◽  
Juntian Zeng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Anja Gulliksen ◽  
Helen Keegan ◽  
Cara Martin ◽  
John O'Leary ◽  
Lars A. Solli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Clinical Sample ◽  
Point Of Care ◽  
Fluorescent Detection ◽  
Clinical Samples ◽  
Microfluidic Channels ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Hpv E6

The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.

scholarly journals Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens

2012 ◽  
Vol 84 (6) ◽  
pp. 906-911 ◽  
Author(s):  
C. Mengelle ◽  
J.-M. Mansuy ◽  
K. Sandres-Sauné ◽  
C. Barthe ◽  
J. Boineau ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Extraction System ◽  
Acid Extraction ◽  
Prospective Evaluation ◽  
Nucleic Acid Extraction ◽  
Respiratory Specimens

scholarly journals Facile silicification of plastic surface for bioassays

2017 ◽  
Vol 53 (13) ◽  
pp. 2134-2137 ◽  
Author(s):  
Seonki Hong ◽  
Ki Soo Park ◽  
Ralph Weissleder ◽  
Cesar M. Castro ◽  
Hakho Lee
Keyword(s):  
Nucleic Acid ◽  
Surface Chemistry ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Plastic Substrates ◽  
Biomimetic Surface ◽  
Plastic Surface

We report a biomimetic surface chemistry to modify plastic substrates for point-of-care nucleic acid extraction.

scholarly journals Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Nasir Ali ◽  
Rita de Cássia Pontello Rampazzo ◽  
Alexandre Dias Tavares Costa ◽  
Marco Aurelio Krieger
Keyword(s):  
Nucleic Acid ◽  
Extraction Method ◽  
Point Of Care ◽  
Extraction Methods ◽  
Vital Role ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Point Of Care Diagnostics ◽  
Working Principle

Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at thepoint of careorpoint of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.

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