scholarly journals Coupling between apical tension and basal adhesion allow epithelia to collectively sense and respond to substrate topography over long distances

2015 ◽  
Vol 7 (12) ◽  
pp. 1611-1621 ◽  
Author(s):  
Kyle E. Broaders ◽  
Alec E. Cerchiari ◽  
Zev J. Gartner
Keyword(s):  
Substrate Topography ◽  
Cell Substrate ◽  
Sense And Respond ◽  
Cell Medium ◽  
Cell Cell

Epithelia have the capacity to sense and respond to substrate topography through the coupling of tensions at the cell–cell, cell–substrate, and cell–medium interfaces.

scholarly journals Real-Time Monitoring of Epithelial Cell-Cell and Cell-Substrate Interactions by Infrared Surface Plasmon Spectroscopy

2010 ◽  
Vol 99 (12) ◽  
pp. 4028-4036 ◽  
Author(s):  
Victor Yashunsky ◽  
Vladislav Lirtsman ◽  
Michael Golosovsky ◽  
Dan Davidov ◽  
Benjamin Aroeti
Keyword(s):  
Epithelial Cell ◽  
Real Time ◽  
Surface Plasmon ◽  
Real Time Monitoring ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Surface Plasmon Spectroscopy ◽  
Cell Cell

Reconstitution of Embryo-Like Structures from Sea Urchin Embryo Cells: Distinction Between Cell-Cell and Cell-Substrate Associations

1982 ◽  
Vol 21 (1-3) ◽  
pp. 79-85 ◽  
Author(s):  
MICHIKO WATANABE ◽  
DONALD R. BERTOLINI ◽  
J. RANDOLPH SCHNTTMAN ◽  
ROBERT S. TURNER
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Embryo ◽  
Cell Substrate ◽  
Embryo Cells ◽  
Cell Cell

scholarly journals Basal-Cell Adhesion Molecule (B-CAM) is Induced in Epithelial Skin Tumors and Inflammatory Epidermis, and is Expressed at Cell–Cell and Cell–Substrate Contact Sites

2000 ◽  
Vol 115 (6) ◽  
pp. 1047-1053 ◽  
Author(s):  
Margarete Schön ◽  
Viktor Hogenkamp ◽  
B. Gregor Wienrich ◽  
Michael P. Schön ◽  
C. Eberhard Klein ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Basal Cell ◽  
Cell Adhesion Molecule ◽  
Skin Tumors ◽  
Contact Sites ◽  
Cell Substrate ◽  
Cell Cell

Spheroid formation of human keratinocyte: Balancing between cell-substrate and cell-cell interaction

2020 ◽  
Vol 76 (2) ◽  
pp. 329-340
Author(s):  
Yan Nie ◽  
Xun Xu ◽  
Weiwei Wang ◽  
Nan Ma ◽  
Andreas Lendlein
Keyword(s):  
Hacat Cell ◽  
Cell Interaction ◽  
Adhesive Force ◽  
Design Criterion ◽  
Spheroid Formation ◽  
Cell Functions ◽  
Substrate Adhesion ◽  
Cell Substrate ◽  
Coated Surfaces ◽  
Cell Cell

BACKGROUND: The formation of spheroids is tightly regulated by intrinsic cell-cell and cell-substrate interactions. OBJECTIVE: The chitosan (CS)-coating was applied to investigate the driven force directed the spheroid formation. METHODS: The effects of CS on cell functions were studied. Atomic force microscopy was employed to measure the cell- biomaterial interplay at single cell level. RESULTS: HaCaT cells shifted from their flattened sheet to a compact 3D spheroidal morphology when increasing CS-coating concentration. The proliferative capacity of HaCaT was preserved in the spheroid. The expression and activation of integrin β1 (ITGB1) were enhanced on CS modified surfaces, while the active to total ratio of ITGB1 was decreased. The adhesive force of a single HaCaT cell to the tissue culture plate (TCP) was 4.84±0.72 nN. It decreased on CS-coated surfaces as CS concentration increased, from 2.16±0.26 nN to 0.96±0.17 nN. The adhesive force between the single HaCaT cell to its neighbor cell increased as CS concentration increased, from 1.15±0.09 nN to 2.60±0.51 nN. CONCLUSIONS: Conclusively, the decreased cell- substrate adhesion was the main driven force in the spheroid formation. This finding might serve as a design criterion for biomaterials facilitating the formation of epithelial spheroids.

scholarly journals Substrate stiffening promotes endothelial monolayer disruption through enhanced physical forces

2011 ◽  
Vol 300 (1) ◽  
pp. C146-C154 ◽  
Author(s):  
Ramaswamy Krishnan ◽  
Darinka D. Klumpers ◽  
Chan Y. Park ◽  
Kavitha Rajendran ◽  
Xavier Trepat ◽  
...  
Keyword(s):  
Physiological Mechanism ◽  
Rho Kinase ◽  
Traction Force ◽  
Vascular Leakage ◽  
Endothelial Monolayer ◽  
Gap Formation ◽  
Cell Substrate ◽  
Physical Forces ◽  
Cell Cell

A hallmark of many, sometimes life-threatening, inflammatory diseases and disorders is vascular leakage. The extent and severity of vascular leakage is broadly mediated by the integrity of the endothelial cell (EC) monolayer, which is in turn governed by three major interactions: cell-cell and cell-substrate contacts, soluble mediators, and biomechanical forces. A potentially critical but essentially uninvestigated component mediating these interactions is the stiffness of the substrate to which the endothelial monolayer is adherent. Accordingly, we investigated the extent to which substrate stiffening influences endothelial monolayer disruption and the role of cell-cell and cell-substrate contacts, soluble mediators, and physical forces in that process. Traction force microscopy showed that forces between cell and cell and between cell and substrate were greater on stiffer substrates. On stiffer substrates, these forces were substantially enhanced by a hyperpermeability stimulus (thrombin, 1 U/ml), and gaps formed between cells. On softer substrates, by contrast, these forces were increased far less by thrombin, and gaps did not form between cells. This stiffness-dependent force enhancement was associated with increased Rho kinase activity, whereas inhibition of Rho kinase attenuated baseline forces and lessened thrombin-induced inter-EC gap formation. Our findings demonstrate a central role of physical forces in EC gap formation and highlight a novel physiological mechanism. Integrity of the endothelial monolayer is governed by its physical microenvironment, which in normal circumstances is compliant but during pathology becomes stiffer.

scholarly journals Physical confinement signals regulate the organization of stem cells in three dimensions

2016 ◽  
Vol 13 (123) ◽  
pp. 20160613 ◽  
Author(s):  
Sebastian V. Hadjiantoniou ◽  
David Sean ◽  
Maxime Ignacio ◽  
Michel Godin ◽  
Gary W. Slater ◽  
...  
Keyword(s):  
Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Cell Interactions ◽  
Three Dimensions ◽  
Cell Substrate ◽  
Cell Cell

During embryogenesis, the spherical inner cell mass (ICM) proliferates in the confined environment of a blastocyst. Embryonic stem cells (ESCs) are derived from the ICM, and mimicking embryogenesis in vitro , mouse ESCs (mESCs) are often cultured in hanging droplets. This promotes the formation of a spheroid as the cells sediment and aggregate owing to increased physical confinement and cell–cell interactions. In contrast, mESCs form two-dimensional monolayers on flat substrates and it remains unclear if the difference in organization is owing to a lack of physical confinement or increased cell–substrate versus cell–cell interactions. Employing microfabricated substrates, we demonstrate that a single geometric degree of physical confinement on a surface can also initiate spherogenesis. Experiment and computation reveal that a balance between cell–cell and cell–substrate interactions finely controls the morphology and organization of mESC aggregates. Physical confinement is thus an important regulatory cue in the three-dimensional organization and morphogenesis of developing cells.

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