scholarly journals Biocatalytic conversion of lignin to aromatic dicarboxylic acids in Rhodococcus jostii RHA1 by re-routing aromatic degradation pathways

2015 ◽  
Vol 17 (11) ◽  
pp. 4974-4979 ◽  
Author(s):  
Zoe Mycroft ◽  
Maria Gomis ◽  
Paul Mines ◽  
Paul Law ◽  
Timothy D. H. Bugg
Keyword(s):  
Dicarboxylic Acid ◽  
Dicarboxylic Acids ◽  
Degradation Pathways ◽  
Gene Insertion ◽  
Pyridine Dicarboxylic Acid ◽  
Aromatic Dicarboxylic Acids ◽  
Aromatic Degradation ◽  
Rhodococcus Jostii Rha1 ◽  
Rhodococcus Jostii

A gene insertion approach is used in Rhodococcus jostii RHA1 to generate pyridine-dicarboxylic acid bioproducts from lignin.

scholarly journals Metabolic engineering of Rhodococcus jostii RHA1 for production of pyridine-dicarboxylic acids from lignin

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Edward M. Spence ◽  
Leonides Calvo-Bado ◽  
Paul Mines ◽  
Timothy D. H. Bugg
Keyword(s):  
Dicarboxylic Acid ◽  
Wheat Straw ◽  
Carbon Sources ◽  
Dicarboxylic Acids ◽  
Wild Type ◽  
Expression Plasmid ◽  
Genes Encoding ◽  
Rhodococcus Jostii Rha1 ◽  
Soda Lignin ◽  
Rhodococcus Jostii

AbstractGenetic modification of Rhodococcus jostii RHA1 was carried out in order to optimise the production of pyridine-2,4-dicarboxylic acid and pyridine-2,5-dicarboxylic acid bioproducts from lignin or lignocellulose breakdown, via insertion of either the Sphingobium SYK-6 ligAB genes or Paenibacillus praA gene respectively. Insertion of inducible plasmid pTipQC2 expression vector containing either ligAB or praA genes into a ΔpcaHG R. jostii RHA1 gene deletion strain gave 2–threefold higher titres of PDCA production from lignocellulose (200–287 mg/L), compared to plasmid expression in wild-type R. jostii RHA1. The ligAB genes were inserted in place of the chromosomal pcaHG genes encoding protocatechuate 3,4-dioxygenase, under the control of inducible Picl or PnitA promoters, or a constitutive Ptpc5 promoter, producing 2,4-PDCA products using either wheat straw lignocellulose or commercial soda lignin as carbon source. Insertion of Amycolatopsis sp. 75iv2 dyp2 gene on a pTipQC2 expression plasmid led to enhanced titres of 2,4-PDCA products, due to enhanced rate of lignin degradation. Growth in minimal media containing wheat straw lignocellulose led to the production of 2,4-PDCA in 330 mg/L titre in 40 h, with > tenfold enhanced productivity, compared with plasmid-based expression of ligAB genes in wild-type R. jostii RHA1. Production of 2,4-PDCA was also observed using several different polymeric lignins as carbon sources, and a titre of 240 mg/L was observed using a commercially available soda lignin as feedstock.

Four cobalt(ii) coordination polymers with diverse topologies derived from flexible bis(benzimidazole) and aromatic dicarboxylic acids: syntheses, crystal structures and catalytic properties

RSC Advances ◽  
2014 ◽  
Vol 4 (106) ◽  
pp. 61281-61289 ◽  
Author(s):  
Xiao Xiao Wang ◽  
Baoyi Yu ◽  
Kristof Van Hecke ◽  
Guang Hua Cui
Keyword(s):  
Dicarboxylic Acid ◽  
Crystal Structures ◽  
Coordination Polymers ◽  
Catalytic Properties ◽  
Hydrothermal Reaction ◽  
Metal Organic Frameworks ◽  
Dicarboxylic Acids ◽  
Metal Organic ◽  
Aromatic Dicarboxylic Acids

Four metal–organic frameworks (MOFs) were obtained from the hydrothermal reaction of Co(ii) and with dicarboxylic acid and flexible bis(5,6-dimethylbenzimidazole) and characterized. The four MOFs exhibit distinct 2D or 3D structural frameworks.

scholarly journals Pyridinedicarboxylic Acid Derivatives of Dichlorobis(cyclopentadienyl)titanium(IV)

2012 ◽  
Vol 9 (1) ◽  
pp. 277-281
Author(s):  
Satish C. Dixit ◽  
Rohit K. Singh
Keyword(s):  
Dicarboxylic Acid ◽  
Spectral Data ◽  
Quinolinic Acid ◽  
Magnetic Moment ◽  
Electrical Conductance ◽  
Dicarboxylic Acids ◽  
Pyridinedicarboxylic Acid ◽  
Pyridine Dicarboxylic Acid ◽  
Elemental Analyses ◽  
Derivatives Of

Reactions of dichlorobis(cyclopentadienyl)titanium(IV) with pyridine dicarboxylic acidsviz. quinolinic acid (QAH2), 2,4-pyridine dicarboxylic acid (PAH2), isocinchomeronic acid (IAH2), cinchomeronic acid (CAH2) and dinicotinic acid (DAH2) were carried out in different stoichiometric ratios. Complexes of the type Cp2Ti(QAH)Cl, Cp2Ti(QAH)2, Cp2Ti(PAH)Cl, Cp2Ti(PAH)2, Cp2Ti(IAH)Cl, Cp2Ti(IAH)2, Cp2Ti(CAH)Cl, Cp2Ti(CAH)2, Cp2Ti(DAH)Cl and Cp2Ti(DAH)2were obtained. These new complexes were characterized on the basis of their elemental analyses, electrical conductance, magnetic moment and spectral data.

IR and 13C, 17O, and 119Sn NMR spectra of some bis(1-butyl)tin(IV) carboxylates of dicarboxylic acids

1991 ◽  
Vol 56 (9) ◽  
pp. 1908-1915 ◽  
Author(s):  
Jaroslav Holeček ◽  
Antonín Lyčka ◽  
Milan Nádvorník ◽  
Karel Handlíř
Keyword(s):  
Infrared Spectroscopy ◽  
Nmr Spectroscopy ◽  
Dicarboxylic Acid ◽  
Nmr Spectra ◽  
Dicarboxylic Acids ◽  
119Sn Nmr ◽  
Carboxylic Groups ◽  
Cyclic Oligomers ◽  
The Media ◽  
Oxygen Atoms

Infrared spectroscopy and multinuclear (13C, 17O, and 119Sn NMR spectroscopy have been used to study the structure of bis(1-butyl)tin(IV) carboxylates of dicarboxylic acids (1-C4H9)2. Sn(X(COO)2), where X = (CH2)n (n = 0-8), CH=CH (cis and trans) and C6H4 (ortho and para).The crystalline compounds are formed by linear or cyclic oligomers or polymers whose basic building units represent a grouping composed of the central tin atom substituted by two 1-butyl groups and coordinated with both oxygen atoms of two anisobidentate carboxylic groups derived from different molecules of a dicarboxylic acid. The environment of the tin atom has a shape of a trapezoidal bipyramid. When dissolvet in non-coordinating solvents, the compounds retain the oligomeric character with unchanged structure of environment of the central tin atom. In the media of coordinating solvents the bis(1-butyl)tin(IV) carboxylates of dicarboxylic acids form complexes whose central hexacoordinated tin atom binds two molecules of the solvent trough their donor atoms. Carboxylic groups form monodenate linkages in these complexes.

Insight into the sugar transport and metabolism in Biphenyl/PCB degrader, Rhodococcus jostii RHA1

2009 ◽  
Vol 108 ◽  
pp. S93
Author(s):  
Naoto Araki ◽  
Toru Suzuki ◽  
Keisuke Miyauchi ◽  
Daisuke Kasai ◽  
Eiji Masai ◽  
...  
Keyword(s):  
Sugar Transport ◽  
Rhodococcus Jostii Rha1 ◽  
Insight Into ◽  
Rhodococcus Jostii
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