Supercritical fluid extraction of grape seeds: extract chemical composition, antioxidant activity and inhibition of nitrite production in LPS-stimulated Raw 264.7 cells

2015 ◽  
Vol 6 (8) ◽  
pp. 2607-2613 ◽  
Author(s):  
Concepción Pérez ◽  
María Luisa Ruiz del Castillo ◽  
Carmen Gil ◽  
Gracia Patricia Blanch ◽  
Gema Flores
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Grape Seeds ◽  
Nitrite Production ◽  
Medicinal Properties ◽  
By Products

Grape by-products are a rich source of bioactive compounds having broad medicinal properties, but are usually wasted from juice/wine processing industries.

scholarly journals Chemical Profile, Antioxidant, Anti-Inflammatory, and Anti-Cancer Effects of Italian Salvia rosmarinus Spenn. Methanol Leaves Extracts

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 826
Author(s):  
Matteo Brindisi ◽  
Chouaha Bouzidi ◽  
Luca Frattaruolo ◽  
Monica R. Loizzo ◽  
Rosa Tundis ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Nitrite Production ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Anti Inflammatory

In this study, we evaluated and compared the chemical composition, the antioxidant, anti-inflammatory, and anti-proliferative effects of four methanol extracts (R1–R4), of Salvia rosmarinus Spenn. in two different sites of Southern Italy obtained by maceration or ultrasound-assisted extraction. Extracts of S. rosmarinus collected on the Ionian coast are indicated with the abbreviations R1 (maceration) and R2 (ultrasound-assisted extraction). Extracts of S. rosmarinus collected on the Tyrrhenian coast are indicated with the abbreviations R3 (maceration) and R4 (ultrasound-assisted extraction). The chemical composition was analyzed using High Pressure liquid chromatography–Diod-Array detection–Electrospray ionization–Quadrupole–Mass Spectroscopy (HPLC-DAD-ESI-Q-MS). The antioxidant activity was analyzed by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and Ferric Reducing Antioxidant Power (FRAP) assays. Antioxidant features were also assessed in lipopolysaccharide (LPS)-stimulated RAW-264.7 murine macrophages, evaluating Reactive Oxygen Species (ROS) production; in the same experimental model, the anti-inflammatory activity of the extracts was investigated. Interestingly, all extracts displayed antioxidant and anti-inflammatory properties. They exhibited significative nitrite production inhibitory activity, whith IC50 values ranging from 3.46 to 5.53 µg/mL, without impairing cell viability. The anti-inflammatory activity was also investigated by Western Blotting and immunofluorescence assay, highlighting the R3 and R4 extracts ability to reduce NF-κB translocation, as well as to disrupt the MAPKs signaling pathway. Extracts exhibited both potential anti-proliferative activity on breast cancer cells, inducing apoptosis, without affecting non-tumorigenic cells, and the ability to inhibit MDA-MB-231 cells’ motility. Finally, the rosemary extracts treatment significantly reduced the power of conditioned media, from MCF-7 or MDA-MB-231 cells to induce nitrite production on RAW 264.7 cells, confirming their promising anti-inflammatory activity.

scholarly journals Chemical Composition, Antioxidant, and Anti-Inflammatory Activity of Essential Oil from Omija (Schisandra chinensis (Turcz.) Baill.) Produced by Supercritical Fluid Extraction Using CO2

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1619
Author(s):  
Jae-Hoon Lee ◽  
Yun-Yeol Lee ◽  
Jangho Lee ◽  
Young-Jin Jang ◽  
Hae-Won Jang
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Inflammatory Activity ◽  
Composition Analysis ◽  
Raw 264.7 ◽  
Schisandra Chinensis ◽  
Fluid Extraction ◽  
Anti Inflammatory

Schisandra chinensis (Turcz.) Baill., which is known as omija in South Korea, is mainly cultivated in East Asia. The present study aimed to investigate the chemical composition of essential oil from the omija (OMEO) fruit obtained by supercritical fluid extraction using CO2 and to confirm the antioxidant and anti-inflammatory activity of OMEO using HaCaT human keratinocyte and RAW 264.7 murine macrophages. As a result of the chemical composition analysis of OMEO using gas chromatography-mass spectrometry, a total of 41 compounds were identified. The detailed analysis results are sesquiterpenoids (16), monoterpenoids (14), ketones (4), alcohols (3), aldehydes (2), acids (1), and aromatic hydrocarbons (1). OMEO significantly reduced the increased ROS levels in HaCaT keratinocytes induced by UV-B irradiation (p < 0.05). It was confirmed that 5 compounds (α-pinene, camphene, β-myrcene, 2-nonanone, and nerolidol) present in OMEO exhibited inhibitory activity on ROS production. Furthermore, OMEO showed excellent anti-inflammatory activity in RAW 264.7 macrophages induced by lipopolysaccharide. OMEO effectively inhibited NO production (p < 0.05) by suppressing the expression of the iNOS protein. Finally, OMEO was investigated for exhibition of anti-inflammatory activity by inhibiting the activation of NF-κB pathway. Taken together, OMEO could be used as a functional food ingredient with excellent antioxidant and anti-inflammatory activity.

Methyl jasmonate treatment of strawberry fruits enhances antioxidant activity and the inhibition of nitrite production in LPS-stimulated Raw 264.7 cells

2013 ◽  
Vol 5 (4) ◽  
pp. 1803-1809 ◽  
Author(s):  
Gema Flores ◽  
Concepción Pérez ◽  
Carmen Gil ◽  
Gracia Patricia Blanch ◽  
María Luisa Ruiz del Castillo
Keyword(s):  
Antioxidant Activity ◽  
Methyl Jasmonate ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Nitrite Production ◽  
Methyl Jasmonate Treatment

Chemical profile and antioxidant activity of sage herbal dust extracts obtained by supercritical fluid extraction

2018 ◽  
Vol 120 ◽  
pp. 305-312 ◽  
Author(s):  
Branimir Pavlić ◽  
Oskar Bera ◽  
Nemanja Teslić ◽  
Senka Vidović ◽  
Giuseppina Parpinello ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Chemical Profile ◽  
Fluid Extraction
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