The Maillard reaction of a shrimp by-product protein hydrolysate: chemical changes and inhibiting effects of reactive oxygen species in human HepG2 cells

2015 ◽  
Vol 6 (6) ◽  
pp. 1919-1927 ◽  
Author(s):  
Fengchao Zha ◽  
Binbin Wei ◽  
Shengjun Chen ◽  
Shiyuan Dong ◽  
Mingyong Zeng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Maillard Reaction ◽  
Hepg2 Cells ◽  
Nutritional Quality ◽  
Protein Hydrolysate ◽  
Reactive Oxygen ◽  
Cellular Damage ◽  
Oxygen Species ◽  
Chemical Changes

A shrimp by-product protein hydrolysate via the Maillard reaction could alleviate cellular damage, but result in higher HMF and loss of nutritional quality.

scholarly journals Cathelicidin Peptides Restrict Bacterial Growth via Membrane Perturbation and Induction of Reactive Oxygen Species

mBio ◽  
2019 ◽  
Vol 10 (5) ◽  
Author(s):  
Dean A. Rowe-Magnus ◽  
Adenine Y. Kao ◽  
Antonio Cembellin Prieto ◽  
Meng Pu ◽  
Cheng Kao
Keyword(s):  
Reactive Oxygen Species ◽  
Antimicrobial Peptides ◽  
Single Cell ◽  
Reactive Oxygen ◽  
Cellular Damage ◽  
Primary Effect ◽  
Gram Negative Bacteria ◽  
Oxygen Species ◽  
Gram Positive ◽  
Gram Negative

ABSTRACT All metazoans produce antimicrobial peptides (AMPs) that have both broad antimicrobial and immunomodulatory activity. Cathelicidins are AMPs that preferentially kill Gram-negative bacteria in vitro, purportedly by assembling into higher-order structures that perforate the membrane. We utilized high-resolution, single-cell fluorescence microscopy to examine their mechanism of action in real time. Engineered cathelicidins rapidly bound to Gram-negative and Gram-positive cells and penetrated the cytoplasmic membrane. Rapid failure of the peptidoglycan superstructure in regions of active turnover caused leakage of cytoplasmic contents and the formation of membrane-bound blebs. A mutation anticipated to destabilize interactions between cathelicidin subunits had no effect on bactericidal activity, suggesting that cathelicidins have activities beyond perforating the membrane. Nanomolar concentrations of cathelicidins, although not bactericidal, reduced the growth rate of Gram-negative and Gram-positive bacteria. The cells exhibited expression changes in multiple essential processes, including protein synthesis, peptidoglycan biosynthesis, respiration, and the detoxification of reactive oxygen species (ROS). Time-lapse imaging revealed that ROS accumulation preceded bleb formation, and treatments that reduced cellular ROS levels overcame these bactericidal effects. We propose that that the primary effect of cathelicidins is to induce the production of ROS that damage bacterial molecules, leading to slowed growth or cell death. Given their low circulating levels in vivo, AMPs may serve to slow bacterial population expansion so that cellular immunity systems can respond to and battle the infection. IMPORTANCE Antimicrobial peptides (AMPs) are an important part of the mammalian innate immune system in the battle against microbial infection. How AMPs function to control bacteria is not clear, as nearly all activity studies use nonphysiological levels of AMPs. We monitored peptide action in live bacterial cells over short time frames with single-cell resolution and found that the primary effect of cathelicidin peptides is to increase the production of oxidative molecules that cause cellular damage in Gram-positive and Gram-negative bacteria.

Generation of Reactive Oxygen Species by Quartz Particles and Its Implication for Cellular Damage

1995 ◽  
Vol 10 (12) ◽  
pp. 1138-1144 ◽  
Author(s):  
Xianglin Shi ◽  
Yan Mao ◽  
Lambert N. Danie ◽  
Umberto Saffiotti ◽  
Nar S. Dalal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Cellular Damage ◽  
Oxygen Species

scholarly journals Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Xiao-Tian Zhang ◽  
Chun-Jiang Yu ◽  
Jian-Wei Liu ◽  
Yan-Ping Zhang ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Palmitic Acid ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Glucose Consumption ◽  
Control Group ◽  
High Dose ◽  
Oxygen Species ◽  
Insulin Resistant

We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P< 0.001); however, QJJ restored HepG2 cell survival (P< 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (allP< 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (allP< 0.05). Furthermore, high-dose QJJ reduced both TNF-αand IL-6 levels as compared to the IR group (allP< 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.

Reactive oxygen species-dependent JNK downregulated olaquindox-induced autophagy in HepG2 cells

2014 ◽  
Vol 35 (7) ◽  
pp. 709-716 ◽  
Author(s):  
Dongxu Zhao ◽  
Congcong Wang ◽  
Shusheng Tang ◽  
Chaoming Zhang ◽  
Shen Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

Neferine induces reactive oxygen species mediated intrinsic pathway of apoptosis in HepG2 cells

2013 ◽  
Vol 136 (2) ◽  
pp. 659-667 ◽  
Author(s):  
Paramasivan Poornima ◽  
Robin Sheeba Quency ◽  
Viswandha Vijaya Padma
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intrinsic Pathway

The inhibition effects of reactive oxygen species by Maillard reaction products in Caco-2 cells

2012 ◽  
Vol 64 (11) ◽  
pp. 921-928 ◽  
Author(s):  
Soo Yeon Chung ◽  
Yong Kwon Lee ◽  
Sung Hee Han ◽  
Seog Won Lee ◽  
Chul Rhee
Keyword(s):  
Reactive Oxygen Species ◽  
Maillard Reaction ◽  
Reactive Oxygen ◽  
Maillard Reaction Products ◽  
Oxygen Species ◽  
Reaction Products
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