Reactive oxygen species-dependent JNK downregulated olaquindox-induced autophagy in HepG2 cells

2014 ◽  
Vol 35 (7) ◽  
pp. 709-716 ◽  
Author(s):  
Dongxu Zhao ◽  
Congcong Wang ◽  
Shusheng Tang ◽  
Chaoming Zhang ◽  
Shen Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Xiao-Tian Zhang ◽  
Chun-Jiang Yu ◽  
Jian-Wei Liu ◽  
Yan-Ping Zhang ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Palmitic Acid ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Glucose Consumption ◽  
Control Group ◽  
High Dose ◽  
Oxygen Species ◽  
Insulin Resistant

We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P< 0.001); however, QJJ restored HepG2 cell survival (P< 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (allP< 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (allP< 0.05). Furthermore, high-dose QJJ reduced both TNF-αand IL-6 levels as compared to the IR group (allP< 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.

Neferine induces reactive oxygen species mediated intrinsic pathway of apoptosis in HepG2 cells

2013 ◽  
Vol 136 (2) ◽  
pp. 659-667 ◽  
Author(s):  
Paramasivan Poornima ◽  
Robin Sheeba Quency ◽  
Viswandha Vijaya Padma
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intrinsic Pathway

The Maillard reaction of a shrimp by-product protein hydrolysate: chemical changes and inhibiting effects of reactive oxygen species in human HepG2 cells

2015 ◽  
Vol 6 (6) ◽  
pp. 1919-1927 ◽  
Author(s):  
Fengchao Zha ◽  
Binbin Wei ◽  
Shengjun Chen ◽  
Shiyuan Dong ◽  
Mingyong Zeng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Maillard Reaction ◽  
Hepg2 Cells ◽  
Nutritional Quality ◽  
Protein Hydrolysate ◽  
Reactive Oxygen ◽  
Cellular Damage ◽  
Oxygen Species ◽  
Chemical Changes

A shrimp by-product protein hydrolysate via the Maillard reaction could alleviate cellular damage, but result in higher HMF and loss of nutritional quality.

scholarly journals Anticancer, Antioxidant Activities and Molecular Docking Study of Thiazolidine -4-one and Thiadiazol Derivatives

2022 ◽  
Author(s):  
Tahseen Abdul qader Alsalim ◽  
Noor M. Nasir ◽  
Amr Ahmed El-Arabey ◽  
Mohnad Abdalla
Keyword(s):  
Reactive Oxygen Species ◽  
Flow Cytometry ◽  
Antioxidant Activity ◽  
Molecular Docking ◽  
Liver Cancer ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Binding Energies ◽  
Oxygen Species ◽  
Hematopoietic Stem

Abstract Liver cancer accounts for a major portion of the global cancer burden. In many nations, the prevalence of this condition has risen in recent decades. New series of thaiazolidinones and thaiadiazolidine have been designed, synthesized, and evaluated for potential antioxidant and antihepatocarcinogenic activity. The antioxidant activity of synthesized compounds was evaluated using a DPPH assay. Furthermore, we examined the compounds against HepG2 cells using MTT assay, flow cytometry analysis through the cell cycle, reactive oxygen species, and apoptosis. The result showed that compound 6b has the highest antioxidant activity with IC50 =60.614±0.739 µM. The anticancer activity showed that compounds 5 and 6b have significant toxicity against liver cancer cells HepG2, IC50 values (9.082 and 4.712) µM, respectively. Flow cytometry experiments revealed that compound 5 arrested HepG2 cells in the S process, while compound 6b arrested HepG2 cells in the G1. Compound 6b had a greater reduction in reactive oxygen species and late apoptosis than compound 5. Substantially, compound 5 had affinity energies of -7.6 and -8.5 for Akt and CDK4 proteins, respectively, but compound 6b had affinity energies of -7.8 and -10.1 for Akt1 and CDK4 proteins, respectively. Consequently, compound 6b had lower binding energies than compound 5. In this work, we used multiple bioinformatics methods to shed light on the prospective therapeutic use of these series as novel candidates to target immune cells in the tumor microenvironment of hepatocellular carcinomas such as CD8+ T cells, endothelial cells, and hematopoietic stem cells. The results of antioxidant, anticancer, molecular docking studies, and bioinformatic analysis showed that compound 6b has a potential impact and could be developed for drug discovery with further research.

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