Peptides present in the non-digestible fraction of common beans (Phaseolus vulgaris L.) inhibit the angiotensin-I converting enzyme by interacting with its catalytic cavity independent of their antioxidant capacity

2015 ◽  
Vol 6 (5) ◽  
pp. 1470-1479 ◽  
Author(s):  
Diego A. Luna-Vital ◽  
Elvira González de Mejía ◽  
Sandra Mendoza ◽  
Guadalupe Loarca-Piña
Keyword(s):  
Phaseolus Vulgaris ◽  
Antioxidant Capacity ◽  
Common Bean ◽  
Antioxidant Activities ◽  
Common Beans ◽  
Converting Enzyme ◽  
Phaseolus Vulgaris L ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme

Pure peptides present in common-bean non-digestible fraction inhibited ACE by interacting with its catalytic cavity, and presented antioxidant activities.

Angiotensin-I converting enzyme inhibitory and antioxidant activities of protein hydrolysates from Phaseolus lunatus and Phaseolus vulgaris seeds

LWT ◽  
2009 ◽  
Vol 42 (10) ◽  
pp. 1597-1604 ◽  
Author(s):  
Juan Torruco-Uco ◽  
Luis Chel-Guerrero ◽  
Alma Martínez-Ayala ◽  
Gloria Dávila-Ortíz ◽  
David Betancur-Ancona
Keyword(s):  
Phaseolus Vulgaris ◽  
Antioxidant Activities ◽  
Protein Hydrolysates ◽  
Phaseolus Lunatus ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme

scholarly journals Black Bean (Phaseolus vulgaris L.) Phenolic Extract Exhibits Antioxidant and Anti-Aging Potential

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 24-24
Author(s):  
David Fonseca Hernandez ◽  
Ignacio Orozco-Avila ◽  
Eugenia Lugo-Cervantes ◽  
Luis Mojica
Keyword(s):  
Phenolic Compounds ◽  
Phaseolus Vulgaris ◽  
Antioxidant Capacity ◽  
Common Bean ◽  
Total Phenolic ◽  
Phaseolus Vulgaris L ◽  
Total Anthocyanin ◽  
Black Bean ◽  
Phenolic Extract ◽  
Inhibitory Potential

Abstract Objectives The objective of this work was to evaluate the potential of common bean phenolic extract to exert anti-aging and antioxidant effect by inhibiting the collagenase, elastase, tyrosinase enzymes and free radicals. Methods 18 varieties of common bean (Phaseolus vulgaris L.) from Chiapas, Mexico, were analyzed for total phenolic content (TPC) and total anthocyanin content (ACN). Supercritical fluid (SCF) and leaching extractions were used for phenolic compounds extraction. Antioxidant capacity was evaluated using DPPH and ABTS scavenging assay. The inhibitory potential of the extract was evaluated for tyrosinase from mushroom, collagenase type-1 from Clostridium histolycum and elastase from porcine pancreas enzymes. Results The TPC ranged from 3.8–34.33 mg GAE/g coat and ACN ranged from 0.04–9.41 mg C3GE/g coat among the 18 common bean varieties (P < 0.05). The cultivar selected for this study was black bean with a TPC of 27.45 ± 0.7 mg GAE/g coat and ACN of 5.3 ± 0.1 mg C3GE/g coat. The best extraction conditions for the obtention of phenolic compounds and anthocyanins were SCF water-ethanol 50% as cosolvent, obtaining 66.60 ± 7.4 mg GAE/g coat (TPC) and 7.3 ± 0.6 mg C3GE/g coat (ACN). TPC and ACN content between each extraction process were statistically different (P < 0.05). For DPPH scavenging assay the IC50 for the black bean extract was 0.32 ± 0.01 mg GAE/g coat, and 0.40 ± 0.03 mg GAE/g coat for ABTS assay. Finally, the IC50 for the enzymatic inhibition assays of tyrosinase, collagenase and elastase were 10.44 ± 1.32, 8.33 ± 0.65 and 0.11 ± 0.02 mg GAE/g coat, respectively. Conclusions Black bean (Phaseolus vulgaris L.) extract presents high antioxidant capacity and inhibitory potential for tyrosinase and metalloproteinases such as collagenase and elastase. Black bean phenolic extracts could be used in cosmeceutical products related to preventing oxidative stress and aging. Funding Sources Author David Fonseca Hernández was supported by a scholarship from Consejo Nacional de Ciencia y Tecnología CONACyT-México, number 901,000. CONACYT-FORDECYT GRANT.

scholarly journals Angiotensin-I-Converting Enzyme Inhibitory and Antioxidant Activities of Protein Hydrolysate from Muscle of Barbel (Barbus callensis)

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Assaad Sila ◽  
Anissa Haddar ◽  
Oscar Martinez-Alvarez ◽  
Ali Bougatef
Keyword(s):  
Antioxidant Activities ◽  
Muscle Protein ◽  
Protein Hydrolysate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme ◽  
Ace Inhibitory

The present study investigated angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities of barbel muscle protein hydrolysate prepared with Alcalase. The barbel muscle protein hydrolysate displayed a high ACE inhibitory activity (CI50=0.92 mg/mL). The antioxidant activities of protein hydrolysate at different concentrations were evaluated using variousin vitroantioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method and reducing power assay. The barbel muscle protein hydrolysate exhibited an important radical scavenging effect and reducing power. These results obtained byin vitrosystems obviously established the antioxidant potency of barbel hydrolysate to donate electron or hydrogen atom to reduce the free radical. Furthermore, these bioactive substances can be exploited into functional foods or used as source of nutraceuticals.

scholarly journals Optimal plot size for experimentation of common beans (Phaseolus vulgaris L.) in the northern region of Minas Gerais, Brazil

2021 ◽  
Vol 53 (2) ◽  
pp. 55-63
Author(s):  
Bruno Vinícius Castro Guimarães ◽  
Abner José De Carvalho ◽  
Ignacio Aspiazú ◽  
Liliane Santana da Silva ◽  
Rafael Rogério Pereira da Silva ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Experimental Evaluation ◽  
Minas Gerais ◽  
Northern Region ◽  
Common Beans ◽  
Phaseolus Vulgaris L ◽  
Plot Size ◽  
Maximum Curvature ◽  
Experimental Plots

The objective was to evaluate the minimum size of experimental plots for the common bean (Phaseolus vulgaris L.) using the modified maximum curvature method. The experiment consisted of a uniformity trial with the cultivar BRSFC-402 sown at a spacing of 0.5 m between plant rows and 10 plants per meter within the row. 20 central rows measuring 20 m in length were considered for measurements, totaling 4,000 plants on an area of 200 m2. Final bean stand (FS), mean number of pods per plant (NPP), mean number of grain per pod (NGP), mean 100-grain weight (M100), and grain yield (kg ha-1) were evaluated. At evaluations, each row with 10 plants was considered a basic unit (0.5 m2), amounting to 400 basic units whose dimensions were combined into 14 plot shapes.  The methods of relative information and modified maximum curvature were used to obtain the best shape and the most appropriate plot size, respectively, for experimental evaluation with common bean. Using these methods, and considering that the optimum plot should enable an efficient evaluation of all evaluated characteristics, the appropriate plot size was five UB (25 plants) in the format with five rows x one UB per row. Highlights Support for experimental evaluation of common beans under edaphoclimatic conditions in the northern region of Minas Gerais Experimental plots with five basic units ensure maximum precision for joint evaluation of the main phenotypic descriptors of common beans. The characteristics mass of 100 grains and productivity were associated with the smallest and the largest plot sizes, respectively.

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