scholarly journals A method for the preservation and determination of welding fume nanoparticles in exhaled breath condensate

2016 ◽  
Vol 3 (2) ◽  
pp. 357-364 ◽  
Author(s):  
Sabrina Gschwind ◽  
Halshka Graczyk ◽  
Detlef Günther ◽  
Michael Riediker
Keyword(s):  
Exhaled Breath Condensate ◽  
Environmental Exposures ◽  
Exhaled Breath ◽  
Welding Fume ◽  
Non Invasive ◽  
Invasive Method ◽  
Inhaled Nanoparticles ◽  
Breath Condensate

Analysis of exhaled breath condensate (EBC) represents a non-invasive method for detecting inhaled nanoparticles (NPs) associated with various occupational and environmental exposures.

Simultaneous and Sensitive Determination of Amphetamine, Codeine and Morphine in Exhaled Breath Condensate, Using Capillary Electrophoresis Coupled with On-line and Off-line Enhancing Methods

2020 ◽  
Vol 16 (7) ◽  
pp. 872-879
Author(s):  
Samin Hamidi
Keyword(s):  
Drugs Of Abuse ◽  
Exhaled Breath Condensate ◽  
Direct Injection ◽  
Forensic Toxicology ◽  
Analytical Signal ◽  
Exhaled Breath ◽  
Concentration Method ◽  
Validation Parameters ◽  
Breath Condensate

Background: Abuse of drugs is associated with several medical, forensic, toxicology and social challenges. “Drugs of abuse” testing is therefore an important issue. Objective: We propose a simple CE-based method for the quantification of amphetamine, codeine and morphine after direct injection of Exhaled Breath Condensate (EBC) by the aid of simple stacking mode and an off-line pre-concentration method. Methods: Using graphene oxide adsorbents, amphetamine, codeine and morphine were extracted from EBC in order to eliminate the proteins and other interferences. In addition to off-line method, an online stacking mode was applied to improve the analytical signal obtained from the instrument. Results: The validation parameters were experimented on the developed method based on the FDA guideline over concentration ranges of 12.5-100, 30-500 and 10-1250 ng/mL associated with amphetamine, codeine and morphine, respectively. Small volumes (around 100 μL) of EBC were collected using a lab-made setup and successfully analyzed using the proposed method where precisions and accuracies (within day and between days) were in accordance with the guideline (recommended less than 15 % for biological samples). The recovery tests were used to evaluate the matrix effect and data (94 to 105 %) showed that the proposed method can be applied in different EBC matrix samplings of subjects. Conclusion: The proposed method is superior for simultaneous determination of amphetamine, codeine and morphine over chromatographic analyses because it is fast and consumes fewer chemicals, with no derivatization step.

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

2020 ◽  
Vol 16 (8) ◽  
pp. 1032-1040
Author(s):  
Laleh Samini ◽  
Maryam Khoubnasabjafari ◽  
Mohamad M. Alimorad ◽  
Vahid Jouyban-Gharamaleki ◽  
Hak-Kim Chan ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Exhaled Breath Condensate ◽  
Low Cost ◽  
Exhaled Breath ◽  
Chromatography Method ◽  
Extraction Solvent ◽  
Drug Concentrations ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations. Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS. Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples. Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

Determination of Verapamil in Exhaled Breath Condensate by Using Microextraction and Liquid Chromatography

2019 ◽  
Vol 15 (5) ◽  
pp. 535-541 ◽  
Author(s):  
Fariba Pourkarim ◽  
Ali Shayanfar ◽  
Maryam Khoubnasabjafari ◽  
Fariborz Akbarzadeh ◽  
Sanaz Sajedi-Amin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Good Precision ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate ◽  
Liquid Microextraction

Background:Developing a simple analysis method for quantification of drug concentration is one of the essential issues in pharmacokinetic and therapeutic drug monitoring studies.Objective:A fast and reliable dispersive liquid-liquid microextraction procedure was employed for preconcentration of verapamil in exhaled breath condensate (EBC) samples and this was followed by the determination with high-performance liquid chromatography-ultraviolet detection.Methods:A reverse-phase high-performance liquid chromatography (RP-HPLC) combined with a dispersive liquid-liquid microextraction method (DLLME) was applied for quantification of verapamil in the EBC samples. The developed method was validated according to FDA guidelines.Results:Under the optimum conditions, the method provided a linear range between 0.07 and 0.8 µg.mL-1 with a coefficient of determination of 0.998. The intra- and inter-day relative standard deviation and relative error values of the method were below 15%, which indicated good precision and accuracy. The proposed method was successfully applied for the analysis of verapamil in two real samples with concentrations of 0.07 and 0.09 µg.mL-1.Conclusion:The established HPLC-UV-DLLME method could be applied for the analysis of verapamil in human EBC samples.

scholarly journals Non-Invasive Infections Diagnosis in Lung Transplant Recipients in Exhaled Breath Condensate

2021 ◽  
Vol 40 (4) ◽  
pp. S63
Author(s):  
E. Ibáñez-Martínez ◽  
M. López-Nogueroles ◽  
M. Alcoriza-Balaguer ◽  
I. Pérez ◽  
M. Roca-Marugán ◽  
...  
Keyword(s):  
Lung Transplant ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Transplant Recipients ◽  
Non Invasive ◽  
Invasive Infections ◽  
Lung Transplant Recipients ◽  
Breath Condensate

Validation of a colorimetric method for determination of paracetamol in exhaled breath condensate

2021 ◽  
Author(s):  
Fariba Pourkarim ◽  
Elaheh Rahimpour ◽  
Maryam Khoubnasabjafari ◽  
Vahid Jouyban-Gharamaleki ◽  
Afshin Gharakhani ◽  
...  
Keyword(s):  
Exhaled Breath Condensate ◽  
Colorimetric Method ◽  
Exhaled Breath ◽  
Breath Condensate

scholarly journals Determination of thiocyanate in exhaled breath condensate

2018 ◽  
Vol 126 ◽  
pp. 334-340 ◽  
Author(s):  
Joshua D. Chandler ◽  
Hamed Horati ◽  
Douglas I. Walker ◽  
Enea Pagliano ◽  
Rabindra Tirouvanziam ◽  
...  
Keyword(s):  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Breath Condensate

scholarly journals Exhaled Breath Condensate pH as a Non-invasive Measure of Inflammation in Non-CF Bronchiectasis

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
A. Shoemark ◽  
R. Wilson
Keyword(s):  
Repeated Measures ◽  
Ex Vivo ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Noninvasive Test ◽  
Need For Treatment ◽  
Non Invasive ◽  
Bronchial Inflammation ◽  
Breath Condensate ◽  
Ciliary Dyskinesia

Bronchiectasis is characterised by neutrophilic bronchial inflammation. Direct measurement of lung inflammation would be useful to assess disease activity, guide need for treatment, and monitor response. The aim of this study was to test whether exhaled breath condensate (EBC) pH, a simple noninvasive test, provides a clinically useful measure of inflammation in the lungs of patients with bronchiectasis. 96 consecutively referred patients were studied when clinically stable, 20 followed up over two years, and a further 22 patients seen during an exacerbation. Subjects breathed tidally for 10 minutes into a condensing chamber (Ecoscreen, Erich Jaeger, Hoechberg, Germany). pH in EBC was measured immediately using a pH probe. In a representative group of 25 patients samples were deaerated with argon gas. This was to control for variations in pH ex vivo by removing CO2. EBC was acidic in bronchiectasis patients () compared to controls () and primary ciliary dyskinesia patients (). pH was related to lung volume but not disease severity. Repeated measures show EBC pH changes with symptoms. EBC is further acidified during an exacerbation of bronchiectasis (), this acidification persists following treatment (). EBC pH is not sufficiently sensitive or specific to monitor patients' health status or provide information to inform acute treatment decisions.

scholarly journals Development of a method for the simultaneous detection of Cr(iii) and Cr(vi) in exhaled breath condensate samples using μLC-ICP-MS

2016 ◽  
Vol 31 (4) ◽  
pp. 924-933 ◽  
Author(s):  
Elizabeth Leese ◽  
Jackie Morton ◽  
Philip H. E. Gardiner ◽  
Vikki A. Carolan
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Exhaled Breath Condensate ◽  
Simultaneous Detection ◽  
Exhaled Breath ◽  
Development Study ◽  
Icp Ms ◽  
Breath Condensate

A method development study describing the first simultaneous determination of Cr(III) and Cr(VI) in an exhaled breath condensate sample.

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