scholarly journals Direct measurement of DNA-mediated adhesion between lipid bilayers

2015 ◽  
Vol 17 (24) ◽  
pp. 15615-15628 ◽  
Author(s):  
S. F. Shimobayashi ◽  
B. M. Mognetti ◽  
L. Parolini ◽  
D. Orsi ◽  
P. Cicuta ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Direct Measurement ◽  
Living Cells ◽  
Solid Substrates ◽  
Multivalent Interactions

Multivalent interactions between deformable mesoscopic units are ubiquitous in biology, where membrane macromolecules mediate the interactions between neighbouring living cells and between cells and solid substrates.

Functionalizing the glycocalyx of living cells with supramolecular guest ligands for cucurbit[8]uril-mediated assembly

2016 ◽  
Vol 52 (44) ◽  
pp. 7146-7149 ◽  
Author(s):  
Emanuela Cavatorta ◽  
Mark L. Verheijden ◽  
Wies van Roosmalen ◽  
Jens Voskuhl ◽  
Jurriaan Huskens ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Living Cells ◽  
Non Fouling

Metabolically presented naphthol ligands on the glycocalyx to trap cells to non-fouling lipid bilayers by heterocomplexation with cucurbit[8]uril and methylviologen.

scholarly journals Direct measurement of catalase activity in living cells and tissue biopsies

2016 ◽  
Vol 470 (1) ◽  
pp. 192-196 ◽  
Author(s):  
Christine N. Scaglione ◽  
Qijin Xu ◽  
V. Krishnan Ramanujan
Keyword(s):  
Direct Measurement ◽  
Catalase Activity ◽  
Living Cells

scholarly journals A Tissue-Like Printed Material

Science ◽  
2013 ◽  
Vol 340 (6128) ◽  
pp. 48-52 ◽  
Author(s):  
Gabriel Villar ◽  
Alexander D. Graham ◽  
Hagan Bayley
Keyword(s):  
Tissue Engineering ◽  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Collective Behavior ◽  
Three Dimensional ◽  
Living Cells ◽  
Emergent Properties ◽  
Living Tissue ◽  
Cohesive Material

Living cells communicate and cooperate to produce the emergent properties of tissues. Synthetic mimics of cells, such as liposomes, are typically incapable of cooperation and therefore cannot readily display sophisticated collective behavior. We printed tens of thousands of picoliter aqueous droplets that become joined by single lipid bilayers to form a cohesive material with cooperating compartments. Three-dimensional structures can be built with heterologous droplets in software-defined arrangements. The droplet networks can be functionalized with membrane proteins; for example, to allow rapid electrical communication along a specific path. The networks can also be programmed by osmolarity gradients to fold into otherwise unattainable designed structures. Printed droplet networks might be interfaced with tissues, used as tissue engineering substrates, or developed as mimics of living tissue.

scholarly journals Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregation from Supported Lipid Bilayer Formation

2018 ◽  
Vol 2 (4) ◽  
pp. 50 ◽  
Author(s):  
Fanny Mousseau ◽  
Evdokia Oikonomou ◽  
Victor Baldim ◽  
Stéphane Mornet ◽  
Jean-François Berret
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Living Cells ◽  
Supported Lipid Bilayers ◽  
Supported Lipid Bilayer ◽  
Mixed Aggregates ◽  
Method Of Continuous Variation ◽  
The Impact ◽  
Analytical Expressions ◽  
Lipid Interaction

The impact of nanomaterials on lung fluids, or on the plasma membrane of living cells, has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Currently, there is a need to have simple protocols that can readily evaluate the structures made from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between experiments and modeling is that electrostatics play a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

scholarly journals Fluorescent conjugates of brefeldin A selectively stain the endoplasmic reticulum and Golgi complex of living cells.

1995 ◽  
Vol 43 (9) ◽  
pp. 907-915 ◽  
Author(s):  
Y Deng ◽  
J R Bennink ◽  
H C Kang ◽  
R P Haugland ◽  
J W Yewdell
Keyword(s):  
Endoplasmic Reticulum ◽  
Lipid Bilayers ◽  
Golgi Complex ◽  
Intracellular Transport ◽  
Brefeldin A ◽  
Living Cells ◽  
Vesicular Trafficking ◽  
Cellular Functions ◽  
Animal Cells ◽  
Specific Localization

The fungal metabolite brefeldin A (BFA) interferes with vesicular trafficking in most animal cells. To gain insight into the mechanism of BFA action, we esterified it to the fluorophore, boron dipyromethene difluoride (BODIPY). BODIPY-BEA localized predominantly in the endoplasmic reticulum (ER) and Golgi complex of viable cells and was extracted by detergent treatment, suggesting it interacts primarily with lipid bilayers. The localization of the conjugate is conferred by BFA, since free BODIPY or BODIPY esterified to cyclopentanol did not specifically localize to internal membranes. BODIPY-BFA exhibited a similar biological activity to BFA, but only when used at higher concentrations and after a delay. HPLC analysis revealed that over this period, cells converted BODIPY-BFA to species co-eluting with free BODIPY and BFA. Therefore, BODIPY-BFA is probably inactive until BFA is released by cellular esterases. The specific localization of BODIPY-BFA to the ER and Golgi complex suggests that BFA might exert its effects on vesicular trafficking by perturbing the lipid bilayer of its target organelles. Because BODIPY-BFA intensely stains the ER at concentrations that have no discernible effects on intracellular transport or other cellular functions, it should be useful for visualizing the ER in living cells.

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