A mitochondria-targeted fluorescent probe for ratiometric detection of endogenous sulfur dioxide derivatives in cancer cells

2016 ◽  
Vol 52 (13) ◽  
pp. 2760-2763 ◽  
Author(s):  
Dong-Peng Li ◽  
Zhao-Yang Wang ◽  
Xiang-Jian Cao ◽  
Jie Cui ◽  
Xin Wang ◽  
...  
Keyword(s):  
Sulfur Dioxide ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Ratiometric Detection

A new mitochondria-targeted fluorescent probe HCy-D, constructed by dansyl and hemicyanine fluorophores, for SO2derivatives (HSO3−/SO32−) was presented.

scholarly journals A new fluorescent probe for colorimetric and ratiometric detection of sulfur dioxide derivatives in liver cancer cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Dong-Peng Li ◽  
Zhao-Yang Wang ◽  
Jie Cui ◽  
Xin Wang ◽  
Jun-Ying Miao ◽  
...  
Keyword(s):  
Sulfur Dioxide ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Liver Cancer Cells ◽  
Ratiometric Detection

Mitochondrial directed ratiometric fluorescent probe for quantitive detection of sulfur dioxide derivatives

2019 ◽  
Vol 43 (13) ◽  
pp. 5255-5259 ◽  
Author(s):  
Congcong Gao ◽  
Yong Tian ◽  
Rubo Zhang ◽  
Jing Jing ◽  
Xiaoling Zhang
Keyword(s):  
Sulfur Dioxide ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
Good Biocompatibility ◽  
Ratiometric Fluorescent Probe

A good biocompatibility fluorescence probe for imaging of sulfur dioxide derivatives in cancer cells.

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

A near-infrared fluorescent probe targeting mitochondria for sulfite detection and its application in food and biology

2021 ◽  
Author(s):  
Jianbin Chao ◽  
Zhuo Wang ◽  
Yongbin Zhang ◽  
Fangjun Huo ◽  
Caixia Yin
Keyword(s):  
Cardiovascular Diseases ◽  
Sulfur Dioxide ◽  
Human Health ◽  
Fluorescent Probe ◽  
Respiratory Diseases ◽  
Near Infrared ◽  
Air Pollutant ◽  
Great Harm

Abstract: Sulfur dioxide (SO2) is the main air pollutant in the environment, causing great harm to human health. Abnormal SO2 levels are usually associated with some respiratory diseases, cardiovascular diseases,...

Tracking lipid droplet dynamics for the discrimination of cancer cells by a solvatochromic fluorescent probe

2021 ◽  
Vol 333 ◽  
pp. 129541
Author(s):  
Linlin Yang ◽  
Jianping Wang ◽  
Bianhua Liu ◽  
Guangmei Han ◽  
Hong Wang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Lipid Droplet ◽  
Droplet Dynamics

Novel diaminomaleonitrile-based fluorescent probe for ratiometric detection and bioimaging of hypochlorite

2018 ◽  
Vol 265 ◽  
pp. 365-370 ◽  
Author(s):  
Yuliang Jiang ◽  
Shishan Wu ◽  
Can Jin ◽  
Bingxiang Wang ◽  
Jian Shen
Keyword(s):  
Fluorescent Probe ◽  
Ratiometric Detection

Insight into sulfur dioxide and its derivatives metabolism in living system with visualized evidences via ultra-sensitive fluorescent probe

2022 ◽  
Vol 423 ◽  
pp. 127179
Author(s):  
Xia Zhang ◽  
Li Zhang ◽  
Shudi Liu ◽  
Xiaozhen Zhu ◽  
Panpan Zhou ◽  
...  
Keyword(s):  
Sulfur Dioxide ◽  
Fluorescent Probe ◽  
Living System ◽  
Insight Into

An “Enhanced PET”-Based Fluorescent Probe with Ultrasensitivity for Imaging Basal and Elesclomol-Induced HClO in Cancer Cells

2014 ◽  
Vol 136 (37) ◽  
pp. 12820-12823 ◽  
Author(s):  
Hao Zhu ◽  
Jiangli Fan ◽  
Jingyun Wang ◽  
Huiying Mu ◽  
Xiaojun Peng
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe
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